PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1237 [new locus tag: SAUSA300_RS06710 ]
• pan locus tag^?: SAUPAN003714000
• symbol: lexA
• pan gene symbol^?: lexA
• synonym:
• product: LexA repressor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1237 [new locus tag: SAUSA300_RS06710 ]
• symbol: lexA
• product: LexA repressor
• replicon: chromosome
• strand: -
• coordinates: 1355029..1355652
• length: 624
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913621 NCBI
• RefSeq: YP_493934 NCBI
• BioCyc: see SAUSA300_RS06710
• MicrobesOnline: 1292752 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAGAGAATTAACAAAACGACAAAGCGAAATATATAACTATATTAAACAAGTTGTTCAA
  ACGAAAGGTTATCCGCCTAGTGTTCGCGAAATTGGTGAAGCAGTTGGCTTAGCATCCAGT
  TCAACTGTTCATGGTCACCTTTCACGTCTTGAAGAAAAAGGCTATATAAGAAGAGATCCA
  ACGAAACCACGTGCTATAGAAATTGTAAGTGATCAAACAAATGATAATATTAATATGGAA
  GAAACGATTCATGTGCCAGTTATTGGTAAAGTCACAGCAGGTGTTCCTATTACCGCAGTA
  GAAAATATTGAAGAATATTTTCCATTACCTGAACACTTAACATCGACACACAATAGCGAC
  ATATTCATATTAAACGTCGTAGGCGACAGTATGATTGAGGCTGGTATATTAGACGGAGAC
  AAAGTAATTGTTCGCAGTCAAACCATAGCAGAAAATGGAGACATTATTGTTGCTATGACT
  GAGGAAGATGAAGCAACTGTCAAACGCTTCTATAAAGAAAAAAATCGTTATCGATTACAA
  CCTGAAAATAGTACAATGGAGCCAATTTACCTAGACAATGTTGCTGTAATTGGGAAAGTA
  ATTGGTTTGTACCGCGAAATGTAA
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  624
  

⊟Protein

⊟General

• locus tag: SAUSA300_1237 [new locus tag: SAUSA300_RS06710 ]
• symbol: LexA
• description: LexA repressor
• length: 207
• theoretical pI: 4.88134
• theoretical MW: 23301.3
• GRAVY: -0.357971

⊟Function

• reaction:
  EC 3.4.21.88?  ExPASy
  Repressor LexA Hydrolysis of Ala-|-Gly bond in repressor LexA
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 250.9)
  Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 250.9)
  and 1 more
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 15.4)
• TheSEED  :
  • SOS-response repressor and protease LexA (EC 3.4.21.88)
  DNA Metabolism DNA repair DNA repair, bacterial  SOS-response repressor and protease LexA (EC 3.4.21.88)
  and 1 more
  DNA Metabolism DNA repair DNA repair, bacterial UmuCD system  SOS-response repressor and protease LexA (EC 3.4.21.88)
• PFAM:
  HTH (CL0123) LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 98.1)
  and 16 more
  Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 65.2)
  HTH (CL0123) HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 24.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 23.1)
  MarR_2; MarR family (PF12802; HMM-score: 19.1)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 17.5)
  MarR; MarR family (PF01047; HMM-score: 17)
  HTH_11; HTH domain (PF08279; HMM-score: 16.4)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 14.2)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.2)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
  Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 13.9)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 13.5)
  RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.5)
  no clan defined KfrA_N; Plasmid replication region DNA-binding N-term (PF11740; HMM-score: 13.4)
  HTH (CL0123) HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 12.5)
  no clan defined CobN-Mg_chel; CobN/Magnesium Chelatase (PF02514; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• stimulus: DNA damage
• genes regulated by LexA, TF important in SOS responseRegPrecise

  repression

  SAUSA300_pUSA030027

  folE < SAUSA300_0714

  uvrB > uvrA

  recA

  lexA

  sbcD > sbcC

  parE > parC

  umuC*

  SAUSA300_1903

  aspB*

  SAUSA300_2129 < SAUSA300_2130 < SAUSA300_2131

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011079
  • TAT(Tat/SPI): 0.002754
  • LIPO(Sec/SPII): 0.001561
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161767 NCBI
• RefSeq: YP_493934 NCBI
• UniProt: Q2FH94 UniProt

⊟Protein sequence

• MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVHGHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVENIEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMTEEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: LexA (repression) regulon

  LexA

  (TF)

  important in SOS response; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications