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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001323
  • pan locus tag?: SAUPAN003714000
  • symbol: lexA
  • pan gene symbol?: lexA
  • synonym:
  • product: transcriptional repressor LexA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001323
  • symbol: lexA
  • product: transcriptional repressor LexA
  • replicon: chromosome
  • strand: -
  • coordinates: 1330864..1331487
  • length: 624
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAGAGAATTAACAAAACGACAAAGCGAAATATATAACTATATTAAACAAGTTGTTCAA
    ATGAAAGGTTATCCGCCTAGTGTTCGCGAAATTGGTGAAGCAGTTGGCTTAGCATCCAGT
    TCAACTGTTCATGGTCACCTTTCACGTCTTGAAGAAAAAGGATATATAAGAAGAGATCCA
    ACGAAACCACGTGCTATAGAAATTGTAAGTGATCAAACAAATGATAATATTAATATGGAA
    GAAACGATTCATGTGCCAGTTATTGGTAAAGTCACAGCAGGTGTTCCTATTACCGCTGTA
    GAAAATATTGAAGAGTATTTTCCATTACCTGAACACTTAACATCGACACATAATAGCGAC
    ATATTCATATTAAACGTCGTAGGCGACAGTATGATTGAGGCTGGTATATTAGACGGAGAC
    AAAGTAATTGTTCGCAGTCAAACCATAGCAGAAAATGGAGACATTATTGTTGCTATGACT
    GAGGAAGATGAAGCAACTGTCAAACGCTTCTATAAAGAAAAAAATCGTTATCGATTACAA
    CCTGAAAATAGTACAATGGAGCCAATTTACCTCGACAATGTTGCTGTAATTGGGAAAGTA
    ATTGGTTTGTACCGCGAAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    624

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001323
  • symbol: LexA
  • description: transcriptional repressor LexA
  • length: 207
  • theoretical pI: 4.88134
  • theoretical MW: 23331.4
  • GRAVY: -0.345411

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.88?  ExPASy
    Repressor LexA Hydrolysis of Ala-|-Gly bond in repressor LexA
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 249.7)
    Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 249.7)
    and 1 more
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 15.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 123.2)
    and 16 more
    HTH (CL0123) LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 96.9)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 24.7)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 23.5)
    MarR_2; MarR family (PF12802; HMM-score: 22.8)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 17.6)
    MarR; MarR family (PF01047; HMM-score: 17.1)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 15.9)
    Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 14.7)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.7)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14.3)
    no clan defined KfrA_N; Plasmid replication region DNA-binding N-term (PF11740; HMM-score: 14.2)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 13.6)
    HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 13.5)
    HTH_TbsP_C; Transcriptional regulator TbsP-like, C-terminal HTH domain (PF23336; HMM-score: 13.2)
    no clan defined CobN-Mg_chel; CobN/Magnesium Chelatase (PF02514; HMM-score: 12.2)
    HTH (CL0123) Xre-like-HTH; Antitoxin Xre-like helix-turn-helix domain (PF20432; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9988
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0008
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010352
    • TAT(Tat/SPI): 0.00253
    • LIPO(Sec/SPII): 0.001251
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVHGHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVENIEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMTEEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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