From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1259 [new locus tag: SAUSA300_RS06840 ]
  • pan locus tag?: SAUPAN003757000
  • symbol: SAUSA300_1259
  • pan gene symbol?: umuC
  • synonym:
  • product: ImpB/MucB/SamB family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1259 [new locus tag: SAUSA300_RS06840 ]
  • symbol: SAUSA300_1259
  • product: ImpB/MucB/SamB family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1386229..1387491
  • length: 1263
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    GTGTATAATTATCATTTATTAGAAGATAGGGATGTTCTATGTATTGACCAAAAAAGTTTT
    TTTGCGAGTGTTTCTTGTATTGAAAAGGGGCTAGATCCATTAGAAACAAAGCTAGCTGTT
    GTTGCAGATACTAAGCGTCAGGGTTCTGTAATATTGGCTGCGACACCTAAATTAAAAGAA
    TTAGGCATCAAGACAGGGTCGCGATTGTTTGAAATACCACATAGAAATGATATTTACATT
    ATCAATCCAAGTATGCGTAAATATCTTAATGTTTCAGTTGCTATTTCTAAGATTGCATTG
    CGTTATATTCCACCTGAAGATTTACACCAATATAGTATTGACGAATTTTTTATGGATGTT
    ACTGATAGCTATCATAGATTTAGTTCTACAGTACATGCATTTTGCGAAAGACTTAAACGT
    GAAATTTATGAAGAAACAGGCATTTATTGTACTGTGGGCATTGGTTCTAATATGTTATTA
    AGTAAAATTGCTATGGATGTTGAAGCGAAGCATAGTCAAAATGGTATAGCTGAATGGCGA
    TATCAAGATGTACCAACGAAATTATGGCCAATTCAGCCCTTGCGAGATTTTTGGGGTATT
    AATCGTCGAACAGAAGCCAAATTGAATAAAAGAGGAATTTTTACTATAGGAGATTTAGCG
    AAATATCCATATAAATTTTTAAAAAAAGAGTTCGGTATTTTAGGTGTTGATATGCATCTA
    CATGCGAATGGGATAGATCAGAGTAAAGTACGTGAAAAGCACAAGATCAGCAATCCATCG
    ATATGCAAAAGTCAAATATTAATGAGAGATTATCATTTTGATGAAGCAAAAGTAGTAATG
    CAAGAGTTAATTGAAGATGTTGCTAGCAGAGTTCGAGCAAGAAAAAAAGTGGCAAGAACG
    ATACATTTTGCCTTTGGCTATAGTGATGAAGGCGGTGTACATAAGCAATATACTTTGAAA
    GATCCAACAAACTTAGAAAAAGATATTTATAAAGTAGTAATGCATTTCGCAGATAAATTA
    TGTAATAAACAAGCACTATATCGTACGCTAAGTATATCTTTGAGTCAATTTATTAATGAG
    GATGAGCGACAGTTAAGTCTGTTTGAAGATGAATACCAACGCAAACGTGACGAATGTCTA
    GCTAAAACGATAGACCAATTACATTTGAAATACGGCAAAGGTATTGTGTCCAAAGCAGTA
    TCGTTTACAGAAGCAGGTACAAAACACGGCAGATTAGGTTTAATGGCTGGACATAAAATG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1259 [new locus tag: SAUSA300_RS06840 ]
  • symbol: SAUSA300_1259
  • description: ImpB/MucB/SamB family protein
  • length: 420
  • theoretical pI: 9.23261
  • theoretical MW: 48426.6
  • GRAVY: -0.393571

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Putative UV-damage repair protein UvrX
    DNA Metabolism DNA repair DNA repair, bacterial  DNA polymerase IV (EC 2.7.7.7)
  • PFAM:
    no clan defined IMS; impB/mucB/samB family (PF00817; HMM-score: 117.2)
    and 2 more
    IMS_C; impB/mucB/samB family C-terminal domain (PF11799; HMM-score: 56.2)
    HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 37.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004875
    • TAT(Tat/SPI): 0.002363
    • LIPO(Sec/SPII): 0.013254
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADTKRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRYIPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLLSKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGDLAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEAKVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVMHFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYGKGIVSKAVSFTEAGTKHGRLGLMAGHKM

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1259&oldid=85373"