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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
- pan locus tag?: SAUPAN005559000
- symbol: SAUSA300_2129
- pan gene symbol?: —
- synonym: trhA
- product: putative hemolysin III
⊟Additional information (user-provided)[edit | edit source]
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
- symbol: SAUSA300_2129
- product: putative hemolysin III
- replicon: chromosome
- strand: -
- coordinates: 2303099..2303782
- length: 684
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913655 NCBI
- RefSeq: YP_494766 NCBI
- BioCyc: see SAUSA300_RS11725
- MicrobesOnline: 1293644 MicrobesOnline
⊟Phenotype[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟DNA sequence[edit | edit source]
- 1
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661ATGTCCAAATCAACTAAAAAAAGTAATGACACAATCGTAGAAACATTTAAGGATATCATC
CCTTTATCATTTGGAGAAGAAATTGGTAATGCGGCATCTCATGGTGTTGCAGCTTTGTTA
ACATTATTAGTGTTACCCTATGCGGCTGTGCACAGTTATATTGCACATGGTACGCTTGCG
TCTGTGAGTATGTCTATTTATGTTATTTCTATCTTTATGATGTTTATTTCATCTACTATT
TATCATTCTATGCAAAATGAAACACCTCATAAATATATTTTAAGGATTATCGATCACAGT
ATGATTTACGTAGCAATTTCTGGTACATATACGCCTATTTTGTTAACAGTTGTAGGTGGT
TGGATAGGTTGGACAGTATTTATTTTATTATGGGGAACGACACTTTGGGGGATTTTATAT
AAATCCATAGCTGTCAAAGTTAACCAAAAGTTAAGCTTAATTGTCTATTTAGTGATGGGC
TGGGTAGGTATTATCTTGTTACCAATTATTTTTATTCGCACATCATGGATATTCATATTA
TTCATTTTCTTAGGTGGTGTGTCTTATACAATTGGTGCATGGTTTTATGCACAAAAGAAT
AGACCATATTTTCATATGATTTGGCATATTTTTATTGTAATAGCATCACTATTGCATTTA
ATTGCAATCTTATATTTTATGTAG60
120
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
- symbol: SAUSA300_2129
- description: putative hemolysin III
- length: 227
- theoretical pI: 9.66153
- theoretical MW: 25675.6
- GRAVY: 0.929515
⊟Function[edit | edit source]
- TIGRFAM: channel protein, hemolysin III family (TIGR01065; HMM-score: 176)
- TheSEED :
- UPF0073 inner membrane protein YqfA
- PFAM: GPCR_A (CL0192) HlyIII; Haemolysin-III related (PF03006; HMM-score: 115.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 7
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9999
- Cell wall & surface Score: 0
- Extracellular Score: 0.0001
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.028901
- TAT(Tat/SPI): 0.004755
- LIPO(Sec/SPII): 0.002572
- predicted transmembrane helices (TMHMM): 7
⊟Accession numbers[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLVLPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMIYVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMGWVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLLHLIAILYFM
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulators: CcpA regulon, LexA (repression) regulon
CcpA (TF) important in Carbon catabolism; RegPrecise transcription unit transferred from N315 data RegPrecise LexA (TF) important in SOS response; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Additional information (user-provided)[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Additional information (user-provided)[edit | edit source]
⊟Other information (user-provided)[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]