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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
- pan locus tag?: SAUPAN005559000
- symbol: NWMN_2071
- pan gene symbol?: —
- synonym:
- product: hemolysin III
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
- symbol: NWMN_2071
- product: hemolysin III
- replicon: chromosome
- strand: -
- coordinates: 2307614..2308300
- length: 687
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331263 NCBI
- RefSeq: YP_001333105 NCBI
- BioCyc:
- MicrobesOnline: 3707673 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGATGTCCAAATCAACTAAAAAAAGTAATGACACAATCGTAGAAACATTTAAGGATATC
ATCCCTTTATCATTTGGAGAAGAAATTGGTAATGCGGCATCTCATGGTGTTGCAGCTTTG
TTAACATTATTAGTGTTACCCTATGCGGCTGTGCACAGTTATATTGCACATGGTACGCTT
GCGTCTGTGAGTATGTCTATTTATGTTATTTCTATCTTTATGATGTTTATTTCATCTACT
ATTTATCATTCTATGCAAAATGAAACACCTCATAAATATATTTTAAGGATTATCGATCAC
AGTATGATTTACGTAGCAATTTCTGGTACATATACGCCTATTTTGTTAACAGTTGTAGGT
GGTTGGATAGGTTGGACAGTATTTATTTTATTATGGGGAACGACACTTTGGGGGATTTTA
TATAAATCCATAGCTGTCAAAGTTAACCAAAAGTTAAGCTTAATTGTCTATTTAGTGATG
GGCTGGGTAGGTATTATCTTGTTACCAATTATTTTTATTCGCACATCATGGATATTCATA
TTATTCATTTTCTTAGGTGGTGTGTCTTATACAATTGGTGCATGGTTTTATGCACAAAAG
AATAGACCATATTTTCATATGATTTGGCATATTTTTATTGTAATAGCATCACTATTGCAT
TTAATTGCAATCTTATATTTTATGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_2071 [new locus tag: NWMN_RS12000 ]
- symbol: NWMN_2071
- description: hemolysin III
- length: 228
- theoretical pI: 9.66153
- theoretical MW: 25806.8
- GRAVY: 0.933772
⊟Function[edit | edit source]
- TIGRFAM: channel protein, hemolysin III family (TIGR01065; HMM-score: 176)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: GPCR_A (CL0192) HlyIII; Haemolysin-III related (PF03006; HMM-score: 104.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 7
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.027156
- TAT(Tat/SPI): 0.003771
- LIPO(Sec/SPII): 0.002182
- predicted transmembrane helices (TMHMM): 7
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLVLPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMIYVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMGWVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLLHLIAILYFM
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulators: CcpA regulon, LexA (repression) regulon
CcpA (TF) important in Carbon catabolism; RegPrecise transcription unit transferred from N315 data RegPrecise LexA (TF) important in SOS response; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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