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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • pan locus tag?: SAUPAN006433000
  • symbol: hisD
  • pan gene symbol?: hisD
  • synonym:
  • product: histidinol dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • symbol: hisD
  • product: histidinol dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2774492..2775748
  • length: 1257
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    TTGCCAATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGAT
    GAGTCATTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAG
    GCTTTGAAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGT
    CATGAACAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAA
    CAAAGTTATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTA
    GAAGAATCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTG
    CCTGGTGGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTA
    GCAGGTGTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAA
    GTATTAGCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAA
    AGTATTGCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGT
    CCAGGCAATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGAC
    CAAATAGCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCC
    ATCGTATATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATT
    AGTGAAGATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAAT
    GTGGACAGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCT
    AGTAATTTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATT
    CAAACAGTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATT
    GGACATTATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCT
    ACAAATAGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAAC
    ACGGTCATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATT
    GCTCATGTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1257

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2702 [new locus tag: SACOL_RS14135 ]
  • symbol: HisD
  • description: histidinol dehydrogenase
  • length: 418
  • theoretical pI: 4.80567
  • theoretical MW: 46406
  • GRAVY: -0.17512

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.23?  ExPASy
    Histidinol dehydrogenase L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
  • TheSEED  :
    • Histidinol dehydrogenase (EC 1.1.1.23)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Histidinol dehydrogenase (EC 1.1.1.23)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Histidinol dehydrogenase (EC 1.1.1.23)
  • PFAM:
    ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 473.9)
    and 8 more
    no clan defined HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
    MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
    DUF4435; Protein of unknown function (DUF4435) (PF14491; HMM-score: 14)
    ZapB; Cell division protein ZapB (PF06005; HMM-score: 13.3)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 13.1)
    no clan defined FadA; Adhesion protein FadA (PF09403; HMM-score: 12)
    YtxH; YtxH-like protein (PF12732; HMM-score: 10.5)
    DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001926
    • TAT(Tat/SPI): 0.000177
    • LIPO(Sec/SPII): 0.000236
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPMLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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