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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2696 [new locus tag: SACOL_RS14105 ]
  • pan locus tag?: SAUPAN006427000
  • symbol: hisI
  • pan gene symbol?: hisI
  • synonym:
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2696 [new locus tag: SACOL_RS14105 ]
  • symbol: hisI
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP
  • replicon: chromosome
  • strand: -
  • coordinates: 2770260..2770892
  • length: 633
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACCAACTATAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAAT
    CAAACAAAACAAGTATTGATGTTGGGTTATATGAACCAAGAAGCTTTTGATAAAACGATA
    GAAGATGGTGTGGTATGTTTCTATTCGCGGTCGAAACAACGGCTATGGACAAAAGGTGAA
    ACATCTGGTCATACGCAACGTGTTAAAGATATTCATGTAGATTGCGACAATGACACTATT
    TTAATTGATGTCATACCAAATGGACCAACATGTCATACAGGCAGTCAAAGTTGTTTCAAC
    ACAGAAGTTCCATTTTCAGTGCAAACATTAGCGCAGACAGTTCAAGATAGTGCCCAATCC
    AATAATGAAAAGTCATATACAAAATATTTATTAACAGAAGGTATAGAAAAGATTACAAAA
    AAATACGGTGAAGAAGCTTTTGAAGTCGTAATTGAAGCAATTAAAGGTGACAAAAAAGCA
    TTTGTAAGTGAAGTAGCAGATGAACTTTATCATTTATTTGTCTTGATGCATGCGCTTGGC
    GTCGATTTTTCAGAAATTGAGGCGGAATTAGCGCGTAGACATCATAAGCGCAATAACTTT
    AAAGGTGAACGACAAAATATCGAACAGTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2696 [new locus tag: SACOL_RS14105 ]
  • symbol: HisI
  • description: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP
  • length: 210
  • theoretical pI: 5.6078
  • theoretical MW: 23972.8
  • GRAVY: -0.526667

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.4.19?  ExPASy
    Phosphoribosyl-AMP cyclohydrolase 1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
    EC 3.6.1.31?  ExPASy
    Phosphoribosyl-ATP diphosphatase 1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family phosphoribosyl-ATP diphosphatase (TIGR03188; EC 3.6.1.31; HMM-score: 90.8)
  • TheSEED  :
    • Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
    • Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
    and 3 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)
  • PFAM:
    no clan defined PRA-CH; Phosphoribosyl-AMP cyclohydrolase (PF01502; HMM-score: 116.5)
    and 5 more
    MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 51.3)
    MazG-like; MazG-like family (PF12643; HMM-score: 18.7)
    MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 15.1)
    Beta_propeller (CL0186) BBS2_Mid; Ciliary BBSome complex subunit 2, middle region (PF14783; HMM-score: 14)
    no clan defined MnmE_helical; MnmE helical domain (PF12631; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003438
    • TAT(Tat/SPI): 0.000189
    • LIPO(Sec/SPII): 0.000425
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGVVCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTEVPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKAFVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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