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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002662a
  • pan locus tag?: SAUPAN006427000
  • symbol: hisIE
  • pan gene symbol?: hisIE
  • synonym:
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002662a
  • symbol: hisIE
  • product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE
  • replicon: chromosome
  • strand: -
  • coordinates: 2681613..2682245
  • length: 633
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq: extdb:JSNZ_002662a NCBI
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACCAAATACAAAATTGATTTTAGCAAAGGTTTAGTGCCAGCAATTTTACAAGATAAT
    CAAACAAAACAAGTATTGATGTTGGGTTATATGAATCAAGAAGCTTTTGATAAAACGATA
    GAAGATGGTGTAGTATGTTTCTATTCGCGGTCGAAACAACGTCTATGGACAAAAGGTGAA
    ACATCTGGTCATACGCAACGTGTTAAAGATATTCATGTAGATTGCGACAATGATACTATT
    TTAATTGATGTCATACCAAATGGACCAACATGTCATACAGGCAGTCAAAGTTGTTTCAAC
    ACAGAAGTTCCATTTTCAGTGCAAACATTAGCGCAGACAGTTCAAGATAGTGCCCAATCC
    AATAATGAAAAGTCATATACAAAATATTTATTAACAGAAGGTATAGAAAAGATTACGAAA
    AAATATGGTGAAGAAGCTTTTGAAGTCGTAATTGAAGCAATTAAAGGTGACAAAAAAGCA
    TTTGTAAGTGAAGTAGCAGATGAACTTTATCATTTATTTGTCTTGATGCATGCGCTAGGC
    GTCGATTTTTCAGAAATTGAGGCAGAATTAGCGCGTAGACATCATAAGCGCAATAACTTT
    AAAGGCGAACGACAAAATATCGAACAGTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002662a
  • symbol: HisIE
  • description: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE
  • length: 210
  • theoretical pI: 5.899
  • theoretical MW: 23986.9
  • GRAVY: -0.528571

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family phosphoribosyl-ATP diphosphatase (TIGR03188; EC 3.6.1.31; HMM-score: 90.8)
  • TheSEED: data available for COL, N315, Newman, USA300_FPR3757
  • PFAM:
    no clan defined PRA-CH; Phosphoribosyl-AMP cyclohydrolase (PF01502; HMM-score: 121.3)
    and 4 more
    MazG (CL0231) PRA-PH; Phosphoribosyl-ATP pyrophosphohydrolase (PF01503; HMM-score: 66.9)
    MazG-like; MazG-like family (PF12643; HMM-score: 20.9)
    Beta_propeller (CL0186) BBS2_Mid; Ciliary BBSome complex subunit 2, middle region (PF14783; HMM-score: 14.9)
    MazG (CL0231) MazG; MazG nucleotide pyrophosphohydrolase domain (PF03819; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9765
    • Cytoplasmic Membrane Score: 0.0009
    • Cell wall & surface Score: 0.0028
    • Extracellular Score: 0.0199
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004252
    • TAT(Tat/SPI): 0.000223
    • LIPO(Sec/SPII): 0.000465
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: extdb:JSNZ_002662a NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGVVCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTEVPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKAFVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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