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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03013
  • pan locus tag?: SAUPAN006433000
  • symbol: SAOUHSC_03013
  • pan gene symbol?: hisD
  • synonym:
  • product: histidinol dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03013
  • symbol: SAOUHSC_03013
  • product: histidinol dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2786404..2787654
  • length: 1251
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
    TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
    AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
    CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
    TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
    TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTGCCTGGT
    GGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTAGCAGGT
    GTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAAGTATTA
    GCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAAAGTATT
    GCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGC
    AATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGACCAAATA
    GCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCCATCGTA
    TATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATTAGTGAA
    GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
    AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
    TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
    GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
    TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
    AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
    ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
    GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_03013
  • symbol: SAOUHSC_03013
  • description: histidinol dehydrogenase
  • length: 416
  • theoretical pI: 4.80567
  • theoretical MW: 46177.7
  • GRAVY: -0.176683

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.23?  ExPASy
    Histidinol dehydrogenase L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
  • TheSEED  :
    • Histidinol dehydrogenase (EC 1.1.1.23)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Histidinol dehydrogenase (EC 1.1.1.23)
    and 1 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Histidinol dehydrogenase (EC 1.1.1.23)
  • PFAM:
    ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 474)
    and 8 more
    no clan defined HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
    DUF4435; Protein of unknown function (DUF4435) (PF14491; HMM-score: 14.2)
    MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
    ZapB; Cell division protein ZapB (PF06005; HMM-score: 13.3)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 13.1)
    no clan defined FadA; Adhesion protein FadA (PF09403; HMM-score: 12)
    YtxH; YtxH-like protein (PF12732; HMM-score: 10.5)
    DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001818
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.000226
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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