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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03009
  • pan locus tag?: SAUPAN006429000
  • symbol: SAOUHSC_03009
  • pan gene symbol?: hisA
  • synonym:
  • product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_03009
  • symbol: SAOUHSC_03009
  • product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2783556..2784260
  • length: 705
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATTGAATTATGGCCAGCGATTGATTTGATTGGGTCAACAAGTGTGAGGTTAACAGAG
    GGTAAATATGATAGTGAAGAAAAAATGTCACGCTCGGCTGAAGAAAGTATTGCTTACTAT
    AGTCAATTTGAATGTGTGAATCGTATTCATATCGTCGACTTGATAGGTGCTAAGGCACAG
    CATGCCCGAGAGTTTGATTATATTAAGTCATTGAGGAGATTAACAACCAAAGATATTGAA
    GTAGGTGGTGGCATTCGTACGAAGTCACAAATCATGGACTACTTTGCCGCAGGGATTAAT
    TATTGCATAGTTGGAACGAAAGGTATTCAAGATACTGATTGGCTTAAAGAGATGGCACAT
    ACATTTCCAGGTCGCATTTATTTATCTGTTGATGCCTATGGAGAAGATATTAAAGTGAAC
    GGATGGGAAGAGGACACAGAGTTAAATTTATTTAGTTTTGTGAGACGGTTATCGGATATA
    CCTCTTGGCGGCATTATATATACTGATATTGCTAAAGATGGCAAAATGTCCGGACCTAAC
    TTTGAATTAACTGGTCAATTAGTAAAGGCAACGACGATTCCTGTCATTGCTTCCGGTGGT
    ATTAGACATCAGCAAGATATTCAACGATTAGCGTCGCTAAATGTTCACGCTGCTATTATA
    GGAAAGGCTGCACATCAAGCATCTTTTTGGGAGGGGCTAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_03009
  • symbol: SAOUHSC_03009
  • description: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
  • length: 234
  • theoretical pI: 5.9599
  • theoretical MW: 26090.5
  • GRAVY: -0.185897

⊟Function[edit | edit source]

  • reaction:
    EC 5.3.1.16?  ExPASy
    1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 248.4)
    and 16 more
    bifunctional HisA/TrpF protein (TIGR01919; EC 5.3.1.16,5.3.1.24; HMM-score: 107)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 76.1)
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 63.7)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 49)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 18.1)
    Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 17.4)
    Metabolism Energy metabolism Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 15.3)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 14)
    putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 13.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 13.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 12.9)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 12.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01306; EC 1.7.1.7; HMM-score: 12)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 11.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.7)
  • TheSEED  :
    • Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)
    and 2 more
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)
    Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)
  • PFAM:
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 222.8)
    and 12 more
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 23.4)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 18.8)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 17.4)
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 16.8)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 16.4)
    Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 15.4)
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 15.2)
    RRM (CL0221) RRM_9; RNA recognition motif (PF18444; HMM-score: 14.7)
    TIM_barrel (CL0036) FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 14.5)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 14.4)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 14)
    PcrB; PcrB family (PF01884; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9874
    • Cytoplasmic Membrane Score: 0.0075
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0049
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011189
    • TAT(Tat/SPI): 0.000709
    • LIPO(Sec/SPII): 0.001951
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFECVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYCIVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDIPLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAAIIGKAAHQASFWEGLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, HisR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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