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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002667
  • pan locus tag?: SAUPAN006433000
  • symbol: hisD
  • pan gene symbol?: hisD
  • synonym:
  • product: histidinol dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002667
  • symbol: hisD
  • product: histidinol dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2685845..2687095
  • length: 1251
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
    TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
    AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
    CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
    TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
    TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTGGGTATTTATGTGCCTGGT
    GGCAAAGCAAGTTATCCATCAACAGTTCTAATGACAGCGACTTTAGCACAAGTAGCAGGT
    GTAGAAAATATTGTTGTTGTGACACCACCTCAACCTAACGGAGTATCCCAAGAGGTATTA
    GCTGCATGTTATATTACGCAAGTTAATCAAGTGTTTCAAGTTGGTGGTGCTCAAAGTATT
    GCTGCATTGACTTATGGAACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGT
    AACCAATTTGTTGCATATGCCAAAAAATATTTATTTGGACAGGTAGGTATTGATCAAATT
    GCAGGACCAACAGAAATAGCACTGATTATTGATGACACCGCAGATTTAGATGCCATCGTA
    TATGATGTATTTGCGCAAGCAGAACATGATGAATTAGCACGTACATATGTCATTAGTGAA
    GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
    AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
    TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
    GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
    TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
    AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
    ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
    GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002667
  • symbol: HisD
  • description: histidinol dehydrogenase
  • length: 416
  • theoretical pI: 4.86448
  • theoretical MW: 46190.7
  • GRAVY: -0.170913

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.23?  ExPASy
    Histidinol dehydrogenase L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 472.3)
    and 6 more
    no clan defined DUF2004; Protein of unknown function (DUF2004) (PF09406; HMM-score: 14.4)
    HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.3)
    MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
    FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 12.8)
    no clan defined YtxH; YtxH-like protein (PF12732; HMM-score: 11.6)
    BORCS6; BLOC-1-related complex sub-unit 6 C-terminal helix (PF10157; HMM-score: 11.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9985
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0009
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001818
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.000226
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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