PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002667
• pan locus tag^?: SAUPAN006433000
• symbol: hisD
• pan gene symbol^?: hisD
• synonym:
• product: histidinol dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002667
• symbol: hisD
• product: histidinol dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2685845..2687095
• length: 1251
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
  TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
  AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
  CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
  TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
  TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTGGGTATTTATGTGCCTGGT
  GGCAAAGCAAGTTATCCATCAACAGTTCTAATGACAGCGACTTTAGCACAAGTAGCAGGT
  GTAGAAAATATTGTTGTTGTGACACCACCTCAACCTAACGGAGTATCCCAAGAGGTATTA
  GCTGCATGTTATATTACGCAAGTTAATCAAGTGTTTCAAGTTGGTGGTGCTCAAAGTATT
  GCTGCATTGACTTATGGAACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGT
  AACCAATTTGTTGCATATGCCAAAAAATATTTATTTGGACAGGTAGGTATTGATCAAATT
  GCAGGACCAACAGAAATAGCACTGATTATTGATGACACCGCAGATTTAGATGCCATCGTA
  TATGATGTATTTGCGCAAGCAGAACATGATGAATTAGCACGTACATATGTCATTAGTGAA
  GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
  AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
  TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
  GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
  TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
  AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
  ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
  GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1251
  

⊟Protein

⊟General

• locus tag: JSNZ_002667
• symbol: HisD
• description: histidinol dehydrogenase
• length: 416
• theoretical pI: 4.86448
• theoretical MW: 46190.7
• GRAVY: -0.170913

⊟Function

• reaction:
  EC 1.1.1.23?  ExPASy
  Histidinol dehydrogenase L-histidinol + H₂O + 2 NAD⁺ = L-histidine + 2 NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 472.3)
  and 6 more
  no clan defined DUF2004; Protein of unknown function (DUF2004) (PF09406; HMM-score: 14.4)
  HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.3)
  MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
  FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 12.8)
  no clan defined YtxH; YtxH-like protein (PF12732; HMM-score: 11.6)
  BORCS6; BLOC-1-related complex sub-unit 6 C-terminal helix (PF10157; HMM-score: 11.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9985
  • Cytoplasmic Membrane Score: 0.0004
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0009
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001818
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000226
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• Operon-mapper  : hisA < hisH < hisB < JSNZ_002666 < hisD < hisG < JSNZ_002669

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)
  CodY	(TF)	important in Amino acid metabolism; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications