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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002664
  • pan locus tag?: SAUPAN006430000
  • symbol: hisH
  • pan gene symbol?: hisH
  • synonym:
  • product: imidazole glycerol phosphate synthase subunit HisH

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002664
  • symbol: hisH
  • product: imidazole glycerol phosphate synthase subunit HisH
  • replicon: chromosome
  • strand: -
  • coordinates: 2683694..2684272
  • length: 579
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAA
    CATTTAGGGTATGAGGTGGTTGTCTCAAATACCTCAAAAATAATCGATCAAGCAGAAACA
    ATCATATTGCCCGGTGTCGGCCATTTTAAAGATGCGATGTCAGAGATAAAACGATTAAAT
    CTCAATGCAATATTGGCTAAGAATACTGATAAGAAGATGATTGGTATTTGTTTAGGCATG
    CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGA
    AATATTTCGCGTATCCAAACAGAATACCCAGTGCCACACTTAGGCTGGAATAATTTAGTG
    AGTAAGCATCCTATGTTAAATCAAGATGTTTATTTCGTACATTCTTATCAAGCGCCGATG
    TCAGAAAATGTCATTGCATATGCGCAGTATGGGGCTGATATTCCGGCAATTGTTCAATTT
    AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATT
    TTGCGTCAGGCAATACAAGGGGGATTTATAAATGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    579

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002664
  • symbol: HisH
  • description: imidazole glycerol phosphate synthase subunit HisH
  • length: 192
  • theoretical pI: 6.13987
  • theoretical MW: 21279.2
  • GRAVY: -0.0348958

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.10?  ExPASy
    non-specified enzyme name
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 239.6)
    and 7 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 29.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 22.3)
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 18.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 15.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 15.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 14.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 13.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 66)
    and 4 more
    SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 24)
    GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 15)
    no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 14.7)
    Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9953
    • Cytoplasmic Membrane Score: 0.0004
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0042
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002578
    • TAT(Tat/SPI): 0.000111
    • LIPO(Sec/SPII): 0.000353
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: HisR* (repression) regulon, CodY (repression) regulon
    HisR*(TF)important in Histidine biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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