PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2467 [new locus tag: SA_RS14125 ]
• pan locus tag^?: SAUPAN006430000
• symbol: hisH
• pan gene symbol^?: hisH
• synonym:
• product: imidazole glycerol phosphate synthase subunit HisH

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2467 [new locus tag: SA_RS14125 ]
• symbol: hisH
• product: imidazole glycerol phosphate synthase subunit HisH
• replicon: chromosome
• strand: -
• coordinates: 2779463..2780041
• length: 579
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125397 NCBI
• RefSeq: NP_375794 NCBI
• BioCyc: see SA_RS14125
• MicrobesOnline: 104820 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAA
  CATTTAGGGTATGAGGTGGTTGTCTCAAATACCTCAAAAATAATCGATCAAGCAGAAACA
  ATCATATTGCCCGGTGTCGGCCATTTTAAAGATGCGATGTCAGAGATAAAACGATTAAAT
  CTCAATGCAATATTGGCTAAGAATACTGATAAGAAGATGATTGGTATTTGTTTAGGCATG
  CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGA
  AATATTTCGCGTATCCAAACAGAATACCCAGTGCCGCACTTAGGTTGGAATAATTTAGTG
  AGTAAGCATCCTATGTTAAATCAAGATGTTTACTTCGTACATTCTTACCAAGCGCCGATG
  TCAGAAAATGTCATTGCATATGCGCAGTATGGGGCTGATATTCCGGCAATTGTTCAATTT
  AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATT
  TTGCGTCAGGCAATACAAGGGGGATTTATAAATGATTGA
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  579
  

⊟Protein

⊟General

• locus tag: SA2467 [new locus tag: SA_RS14125 ]
• symbol: HisH
• description: imidazole glycerol phosphate synthase subunit HisH
• length: 192
• theoretical pI: 6.13987
• theoretical MW: 21279.2
• GRAVY: -0.0348958

⊟Function

• reaction:
  EC 2.4.2.-?  ExPASy
  5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H₂O?
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 239.6)
  and 7 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 29.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 22.3)
  glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 18.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 15.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 15.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 14.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 13.5)
• TheSEED  :
  • Imidazole glycerol-phosphate synthase amidotransferase subunit HisH (EC 4.3.2.10)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)
  and 1 more
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)
• PFAM:
  Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 64.1)
  and 4 more
  SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 23.7)
  GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 15.6)
  no clan defined UPF0180; Uncharacterised protein family (UPF0180) (PF03698; HMM-score: 15.4)
  Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002578
  • TAT(Tat/SPI): 0.000111
  • LIPO(Sec/SPII): 0.000353
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928261 NCBI
• RefSeq: NP_375794 NCBI
• UniProt: P64364 UniProt

⊟Protein sequence

• MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisI < hisF < SA2466 < hisH < hisB < SA2469 < SA2470 < hisG < hisZ

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications