PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS14135 [old locus tag: SACOL2702 ]
• pan locus tag^?: SAUPAN006433000
• symbol: SACOL_RS14135
• pan gene symbol^?: hisD
• synonym:
• product: histidinol dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS14135 [old locus tag: SACOL2702 ]
• symbol: SACOL_RS14135
• product: histidinol dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2774492..2775742
• length: 1251
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2774492..2775742) NCBI
• BioCyc: SACOL_RS14135 BioCyc
• MicrobesOnline: see SACOL2702

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCTTAATGCACAACAATTTTTAAATCAATTTTCATTAGAAGCACCATTAGATGAGTCA
  TTGTATCCAATTATTCGCGATATTTGTCAGGAAGTTAAAGTTCATGGAGATAAGGCTTTG
  AAAATGTATAATCTAACATTCGATCATACGAAAACAGATCATTTAGAAATTAGTCATGAA
  CAAATTAAAGCAGCATTTGACACATTAGATGAAAAAACAAAACAAGCATTACAACAAAGT
  TATGAAAGAATTAAAGCATATCAAGAAAGTATTAAACAGACGAATCAACAGTTAGAAGAA
  TCAGTGGAGTGTTATGAAATATACCATCCACTAGAAAGTGTCGGTATTTATGTGCCTGGT
  GGTAAAGCAAGTTATCCTTCAACGGTATTAATGACAGCGACGTTAGCACAAGTAGCAGGT
  GTGGAAAATATTGTCGTTGTGACACCACCTCAACCTAATGGTGTGTCTCAAGAAGTATTA
  GCAGCATGCTATATTACGCAAGTTGATCAAGTATTTCAAGTTGGTGGTGCTCAAAGTATT
  GCTGCGTTAACTTATGGCACAGAAACGATACCTAAAGTTGATAAGATTGTAGGTCCAGGC
  AATCAATTTGTTGCATATGCCAAGAAATATTTATTTGGACAAGTAGGTATTGACCAAATA
  GCTGGACCAACAGAAATAGCACTTATTATTGATGAAACAGCAGATTTAGATGCCATCGTA
  TATGATGTTTTTGCACAAGCAGAACATGATGAATTAGCACGTACATATGCCATTAGTGAA
  GATGCGCAAGTCCTTAAAGATTTAGAATCACGTATTGCTAAAGCATTGCCTAATGTGGAC
  AGATACGACATTGTTTCTAAAAGTATCGCTAATCAACACTACCTTATCCATGCTAGTAAT
  TTTGATGAAGCATGCCATGTCATGAATACAATCGCGCCTGAACATGCGTCGATTCAAACA
  GTAAATCCTCAACCATATATTGAAAAAGTGAAATATGTGGGTGCATTGTTTATTGGACAT
  TATTCGCCAGAGGTCATAGGAGATTACGTTGCAGGTCCAAGTCATGTATTACCTACAAAT
  AGAACAGCTAGATTTACCAATGGGTTATCGGTCAATGATTTCTTAACACGGAACACGGTC
  ATCCATTTATCAAAAGATACGTTTGAACAAATTGCTGATTCAGCACAACATATTGCTCAT
  GTTGAAGCATTATACAATCACCAGCAGTCTATTTTAATACGTCAGTCTTAG
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⊟Protein

⊟General

• locus tag: SACOL_RS14135 [old locus tag: SACOL2702 ]
• symbol: SACOL_RS14135
• description: histidinol dehydrogenase
• length: 416
• theoretical pI: 4.80567
• theoretical MW: 46177.7
• GRAVY: -0.176683

⊟Function

• reaction:
  EC 1.1.1.23?  ExPASy
  Histidinol dehydrogenase L-histidinol + H₂O + 2 NAD⁺ = L-histidine + 2 NADH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family histidinol dehydrogenase (TIGR00069; EC 1.1.1.23; HMM-score: 479.1)
• TheSEED: see SACOL2702
• PFAM:
  ALDH-like (CL0099) Histidinol_dh; Histidinol dehydrogenase (PF00815; HMM-score: 474)
  and 8 more
  no clan defined HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 14.7)
  DUF4435; Protein of unknown function (DUF4435) (PF14491; HMM-score: 14.2)
  MbeB_N; MbeB-like, N-term conserved region (PF04837; HMM-score: 14)
  ZapB; Cell division protein ZapB (PF06005; HMM-score: 13.3)
  YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 13.1)
  no clan defined FadA; Adhesion protein FadA (PF09403; HMM-score: 12)
  YtxH; YtxH-like protein (PF12732; HMM-score: 10.5)
  DUF4404; Domain of unknown function (DUF4404) (PF14357; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001818
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000226
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446853387 NCBI
• RefSeq: WP_000930643 NCBI
• UniProt: see SACOL2702

⊟Protein sequence

• MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYNLTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESVECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVLAACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGIDQIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKALPNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVGALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIADSAQHIAHVEALYNHQQSILIRQS

⊟Experimental data

• experimentally validated:
• protein localization: see SACOL2702
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, HisR* see SACOL2702

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications