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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1499 [new locus tag: SA_RS08435 ]
  • pan locus tag?: SAUPAN004291000
  • symbol: tig
  • pan gene symbol?: tig
  • synonym:
  • product: trigger factor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1499 [new locus tag: SA_RS08435 ]
  • symbol: tig
  • product: trigger factor
  • replicon: chromosome
  • strand: -
  • coordinates: 1707554..1708855
  • length: 1302
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAGCAACTTGGGAAAAAAAGGAAGGTAACGAAGGTTTATTAACTGTTACTGTTCCT
    GCAGAAAAAGTAAACAAAGCGTTAGATCAAGCATTCAAAAAAGTGGTTAAACAAATTAAC
    GTACCTGGATTCCGTAAAGGTAAAGTGCCACGTCCAATTTTTGAACAACGCTTTGGTGTA
    GAAGCATTATATCAAGATGCTATCGACATTTTATTACCAGATGCTTATGGTGAAGCAATT
    GACGAAACTGATATTAAACCAGTTGCACAACCAGAAGTAAGTGTTACTCAAATTGAAAAA
    GGTAAAGATTTCATTTTTGAAGCAACAGTTACAGTTGAGCCAGAAGTTAAATTAGGAGAC
    TATAAAGGTCTTGAAATTGAAAAACAAGAAACTGAATTATCTGATGATGAGTTACAAGAA
    GCGATTGACCACAGCTTAGGACATTTAGCTGAAATGGTAGTTAAAGAAGATGGTGTTGTT
    GAAAATGGCGACACAGTTAACATTGACTTTAGTGGTTCAGTTGACGGAGAAGAATTCGAA
    GGTGGACAAGCTGAAGGTTACGATTTAGAAATCGGTTCAGGTTCATTCATACCTGGTTTC
    GAAGAGCAATTAGAAGGTATGAAAGTTGACGAAGAAAAAGATGTTGTCGTAACATTCCCA
    GAAGAATACCATGCTGAAGAATTAGCTGGTAAAGAAGCAACTTTCAAAACAAAAGTTAAC
    GAAATTAAATTTAAAGAAGTTCCAGAATTAACAGATGAAATTGCTAATGAATTAGATGCA
    GAAGCAAATACAGTAGACGAGTACAAAGAAAACTTACGTAAACGTTTAGCTGAACAAAAA
    GCTACAGATGCTGAAAATGTTGAAAAAGAAGAAGCGATTACAAAAGCTACTGATAATACA
    ACAATCGATATTCCTGAAGCAATGGTTAATACTGAATTAGATCGTATGGTGTCTGAATTT
    GCACAAAGAATTCAACAACAAGGTTTAGATTTACAAACGTACTTCCAAATCTCAGGTCAA
    GATGAAACTCAATTAAGAGAGCAAATGAAAGACGATGCAGAACAACGTGTTAAAACTAAC
    TTAACTTTAACTGCGATCGCTGAAGCTGAAAAAATCGAAGCTACTGATGAAGATATCGAT
    AAAGAATTAGAAAAAATGAGTAAACAATTTAATATCTCAGTTGAAGATATCAAAAATACT
    TTAGGTAATACTGATATCATTAAAAATGATGTTCGTATCCAAAAAGTTATCGATTTATTA
    AGAGATAACGCAAAGTTCGTTGAAGGAACTAAAGAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1302

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1499 [new locus tag: SA_RS08435 ]
  • symbol: Tig
  • description: trigger factor
  • length: 433
  • theoretical pI: 4.05625
  • theoretical MW: 48608.6
  • GRAVY: -0.642956

⊟Function[edit | edit source]

  • reaction:
    EC 5.2.1.8?  ExPASy
    Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization trigger factor (TIGR00115; EC 5.2.1.8; HMM-score: 451.8)
    and 1 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(hydroxyalkanoate) granule-associated protein (TIGR01837; HMM-score: 8.2)
  • TheSEED  :
    • Trigger factor (EC 5.2.1.8)
    Bacterial Cell Division  Cell division trigger factor (EC 5.2.1.8)
    and 1 more
    RP Bacterial Cell Division  Cell division trigger factor (EC 5.2.1.8)
  • PFAM:
    no clan defined Trigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 156.9)
    Trigger_C (CL0262) Trigger_C; Bacterial trigger factor protein (TF) C-terminus (PF05698; HMM-score: 153.6)
    and 3 more
    FKBP (CL0487) FKBP_C; FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254; HMM-score: 57.6)
    HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 13.1)
    no clan defined DUF4854; Domain of unknown function (DUF4854) (PF16146; HMM-score: 8.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003665
    • TAT(Tat/SPI): 0.000647
    • LIPO(Sec/SPII): 0.001578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGFRKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGKDFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVVENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVTFPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRLAEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTYFQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNISVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1029(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA2035(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA2040(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA0352(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SA2025(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    SA2038(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA1032hypothetical protein  [1] (data from MRSA252)
    SA1562hypothetical protein  [1] (data from MRSA252)
    SA2099hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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