PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001659
• pan locus tag^?: SAUPAN004291000
• symbol: tig
• pan gene symbol^?: tig
• synonym:
• product: trigger factor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001659
• symbol: tig
• product: trigger factor
• replicon: chromosome
• strand: -
• coordinates: 1693714..1695015
• length: 1302
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGACAGCAACTTGGGAAAAAAAGGAAGGTAACGAAGGTTTATTAACTGTTACTGTTCCT
  GCAGAAAAAGTAAACAAAGCGTTAGATCAAGCATTCAAAAAAGTGGTTAAACAAATTAAC
  GTACCTGGATTCCGTAAAGGTAAAGTGCCACGTCCAATTTTTGAACAACGCTTTGGTGTA
  GAAGCATTATATCAAGATGCTATCGACATTTTATTACCAGATGCTTATGGTGAAGCAATT
  GACGAAACTGATATTAAACCAGTTGCACAACCAGAAGTAAGTGTTACTCAAATTGAAAAA
  GGTAAAGATTTCATTTTTGAAGCAACAGTTACAGTTGAGCCAGAAGTTAAATTAGGAGAC
  TATAAAGGTCTTGAAATTGAAAAACAAGAAACTGAATTATCTGATGATGAGTTACAAGAA
  GCGATTGACCACAGCTTAGGACATTTAGCTGAAATGGTCGTTAAAGAAGATGGTGTTGTT
  GAAAATGGCGACACAGTTAACATCGACTTTAGTGGTTCAGTTGACGGAGAAGAATTCGAA
  GGTGGACAAGCTGAAGGTTACGATTTAGAAATCGGTTCAGGTTCATTCATACCTGGTTTC
  GAAGAGCAATTAGAAGGTATGAAAGTTGACGAAGAAAAAGATGTTGTCGTAACATTCCCA
  GAAGAATACCATGCTGAAGAATTAGCCGGTAAAGAAGCAACTTTCAAAACAAAAGTTAAC
  GAAATTAAATTTAAAGAAGTCCCAGAATTAACAGATGAAATTGCTAATGAATTAGATGCA
  GAAGCAAATACAGTAGACGAGTACAAAGAAAACTTACGTAAACGTTTAGCTGAACAAAAA
  GCTACAGATGCTGAAAATGTTGAAAAAGAAGAAGCGATTACAAAAGCTACTGATAATACA
  ACAATCGATATTCCTGAAGCAATGGTTAATACTGAATTAGATCGTATGGTGTCTGAATTT
  GCACAAAGAATTCAACAACAAGGTTTAGATTTACAAACGTACTTCCAAATCTCAGGTCAA
  GATGAAACTCAATTAAGAGAGCAAATGAAAGACGATGCAGAACAACGTGTTAAAACTAAC
  TTAACTTTAACTGCGATCGCTGAAGCTGAAAAAATCGAAGCTACTGATGAAGATATCGAT
  AAAGAATTAGAAAAAATGAGTAAACAATTTAATATCTCAGTTGAAGATATCAAAAATACT
  TTAGGTAATACTGATATCATTAAAAATGATGTTCGTATCCAAAAAGTTATCGATTTATTA
  AGAGATAACGCAAAGTTCGTTGAAGGAACTAAAGAAGATTAA
  60
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  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1302
  

⊟Protein

⊟General

• locus tag: JSNZ_001659
• symbol: Tig
• description: trigger factor
• length: 433
• theoretical pI: 4.05625
• theoretical MW: 48608.6
• GRAVY: -0.642956

⊟Function

• reaction:
  EC 5.2.1.8?  ExPASy
  Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
• TIGRFAM:
  Genetic information processing Protein fate Protein folding and stabilization trigger factor (TIGR00115; EC 5.2.1.8; HMM-score: 451.8)
  and 1 more
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(hydroxyalkanoate) granule-associated protein (TIGR01837; HMM-score: 8.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  no clan defined Trigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 172.6)
  Trigger_C (CL0262) Trigger_C; Bacterial trigger factor protein (TF) C-terminus (PF05698; HMM-score: 159.4)
  and 7 more
  FKBP (CL0487) FKBP_C; FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254; HMM-score: 69.4)
  EF_hand (CL0220) EF-hand_14; EF-hand domain (PF17959; HMM-score: 13.6)
  HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12.7)
  E-set (CL0159) DUF7350; Domain of unknown function (DUF7350) (PF24041; HMM-score: 12.6)
  TIM_barrel (CL0036) GH123_cat; Glycoside hydrolase 123, catalytic domain (PF13320; HMM-score: 9)
  XRCC4_N (CL0777) XRCC4; XRCC4 N-terminal domain (PF06632; HMM-score: 8.6)
  Trigger_C (CL0262) SurA_N_3; SurA-like N-terminal domain (PF13624; HMM-score: 8.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9993
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0005
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003665
  • TAT(Tat/SPI): 0.000647
  • LIPO(Sec/SPII): 0.001578
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGFRKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGKDFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVVENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVTFPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRLAEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTYFQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNISVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications