From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05325 [old locus tag: NWMN_0950 ]
  • pan locus tag?: SAUPAN003304000
  • symbol: NWMN_RS05325
  • pan gene symbol?: ptsI
  • synonym:
  • product: phosphoenolpyruvate--protein phosphotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS05325 [old locus tag: NWMN_0950 ]
  • symbol: NWMN_RS05325
  • product: phosphoenolpyruvate--protein phosphotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1055101..1056819
  • length: 1719
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1055101..1056819) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_0950

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTAT
    TTATTAGTTGAGCCAGACTTAACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGA
    GAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAGTTGAGTTAACTAAAATTAGA
    AATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
    GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCT
    AATGCTGCTACAGCATTAACGGATGTAACAACACAATTTGTTACAATTTTTGAATCTATG
    GATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCGACGTTTCTAAACGTGTGTTA
    TCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATTGATGAAAGCGTTGTTATT
    GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGT
    TTTGCTACAAACATTGGCGGAAGAACAAGTCACTCTGCAATTATGAGTCGTTCTTTAGAA
    ATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAGTTAAACAAGGCGACATGATT
    ATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
    TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGAT
    GCTGATACTGTTACAGTTGATGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCT
    AATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTATCGGCTTATATAGAACTGAG
    TTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAACAATTTGAAGCTTATAAA
    GAAGTATTAGAAGCAATGGGCGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGA
    GATAAAGAATTATCATACTTAAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTACCGT
    GCGATTCGTTTATGCCTTGCGCAACAAGATATTTTCAGACCACAGCTACGTGCATTATTA
    CGTGCATCAGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
    TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCAT
    GACATTTCGGATGATATAGAATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTA
    GCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCGGTACAAATGATTTAATTCAA
    TACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
    CCTTCAATCTTACGTTTAGTTAAACAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGG
    ACAGGTATGTGTGGTGAAATGGCTGGAGATGAAACAGCTATTCCATTATTGCTTGGTTTA
    GGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGAAAGCAAGAAGACAAATTAAT
    GGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
    GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1719

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS05325 [old locus tag: NWMN_0950 ]
  • symbol: NWMN_RS05325
  • description: phosphoenolpyruvate--protein phosphotransferase
  • length: 572
  • theoretical pI: 4.38638
  • theoretical MW: 63218.6
  • GRAVY: -0.174126

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.3.9?  ExPASy
    Phosphoenolpyruvate--protein phosphotransferase Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
  • TIGRFAM:
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 816.2)
    and 2 more
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 209.3)
    Metabolism Energy metabolism Other pyruvate, phosphate dikinase (TIGR01828; EC 2.7.9.1; HMM-score: 135.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PK_TIM (CL0151) PEP-utilizers_C; PEP-utilising enzyme, TIM barrel domain (PF02896; HMM-score: 426.5)
    and 2 more
    no clan defined PEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 111.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 83.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010447
    • TAT(Tat/SPI): 0.000492
    • LIPO(Sec/SPII): 0.00181
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIELVNNYVK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_RS05325&oldid=81199"