PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1753 [new locus tag: NWMN_RS10025 ]
pan locus tag^?: SAUPAN004840000
symbol: NWMN_1753
pan gene symbol^?: perR
• synonym:
• product: transcriptional regulator Fur family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1753 [new locus tag: NWMN_RS10025 ]
• symbol: NWMN_1753
• product: transcriptional regulator Fur family protein
• replicon: chromosome
• strand: -
• coordinates: 1963955..1964401
• length: 447
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331639 NCBI
• RefSeq: YP_001332787 NCBI
• BioCyc:
• MicrobesOnline: 3707341 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGTGTTGAAATAGAATCAATTGAACATGAACTAGAAGAATCAATTGCATCATTGCGA
  CAAGCAGGCGTAAGAATTACACCTCAAAGACAAGCAATATTACGTTATTTAATTTCTTCA
  CATACTCATCCAACAGCTGATGAAATTTATCAAGCACTTTCACCTGATTTTCCAAATATA
  AGTGTTGCGACAATATATAATAACTTAAGAGTGTTTAAAGATATTGGAATTGTAAAAGAA
  TTAACATATGGAGACTCATCAAGTCGATTCGACTTTAATACACATAATCATTATCATATT
  ATATGTGAACAATGTGGTAAGATTGTTGATTTTCAATATCCACAGTTAAATGAAATTGAA
  AGATTAGCTCAGCATATGACTGACTTTGACGTAACACATCATCGAATGGAAATTTATGGA
  GTTTGTAAAGAATGCCAAGATAAATAA
  60
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  420
  447
  

⊟Protein

⊟General

• locus tag: NWMN_1753 [new locus tag: NWMN_RS10025 ]
• symbol: NWMN_1753
• description: transcriptional regulator Fur family protein
• length: 148
• theoretical pI: 5.47242
• theoretical MW: 17183.2
• GRAVY: -0.421622

⊟Function

• TIGRFAM:
• TheSEED  :
  • Peroxide stress regulator PerR, FUR family
  Stress Response Oxidative stress Oxidative stress  Peroxide stress regulator PerR, FUR family
• ⊞PFAM:
  HTH (CL0123) FUR; Ferric uptake regulator family (PF01475; HMM-score: 132.9)
  and 11 more
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 24.2)
  HTH_11; HTH domain (PF08279; HMM-score: 14.9)
  no clan defined Eplus_motif; E+ motif (PF20430; HMM-score: 14.6)
  HEF_HK; HEF_HK domain (PF19191; HMM-score: 13.7)
  DUF2039; Uncharacterized conserved protein (DUF2039) (PF10217; HMM-score: 13.6)
  E6; Early Protein (E6) (PF00518; HMM-score: 13.2)
  Tbcl_zf (CL0839) zf-dskA_traR; Prokaryotic dksA/traR C4-type zinc finger (PF01258; HMM-score: 13.1)
  no clan defined DASH_Hsk3; DASH complex subunit Hsk3 like (PF08227; HMM-score: 13.1)
  HTH (CL0123) HTH_32; Homeodomain-like domain (PF13565; HMM-score: 12.5)
  Zn_Beta_Ribbon (CL0167) Zn_Ribbon_TF; TFIIB zinc-binding (PF08271; HMM-score: 11.8)
  Ribosomal_L37e; Ribosomal protein L37e (PF01907; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors: Hydrogen peroxide; Iron ion (Fe2+) and Manganese ion (Mn2+) act as corepressor
• ⊞genes regulated by PerR*, TF important in Oxidative stress responseRegPrecise

  repression

  ahpF < ahpC

  sufC > sufD > sufS > nifU

  sufB

  katA

  hemY < hemH < hemE

  perR*

  NWMN_1754 < bcp*

  ftnA*

  dps*

  transcription units transferred from N315 data RegPrecise

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8939
  • Cytoplasmic Membrane Score: 0.0034
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.1025
• ⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009002
  • TAT(Tat/SPI): 0.003688
  • LIPO(Sec/SPII): 0.001527
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221965 NCBI
• RefSeq: YP_001332787 NCBI
• UniProt: A0A0H3K987 UniProt

⊟Protein sequence

• MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHPTADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIICEQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1753 < NWMN_1754 < NWMN_1755

⊟Regulation

• ⊞regulators: FapR* (repression) regulon, PerR* (repression) regulon

  FapR*	(TF)	important in Fatty acid biosynthesis; RegPrecise
  PerR*	(TF)	important in Oxidative stress response; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications