PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0789 [new locus tag: NWMN_RS04460 ]
• pan locus tag^?: SAUPAN002899000
• symbol: sufB
• pan gene symbol^?: sufB
• synonym:
• product: FeS assembly protein SufB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0789 [new locus tag: NWMN_RS04460 ]
• symbol: sufB
• product: FeS assembly protein SufB
• replicon: chromosome
• strand: +
• coordinates: 878549..879946
• length: 1398
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332203 NCBI
• RefSeq: YP_001331823 NCBI
• BioCyc:
• MicrobesOnline: 3706338 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGCTAAAAAAGCACCTGATGTTGGGGATTATAAATATGGATTCCACGACGATGATGTG
  TCCATTTTCAGATCAGAACGTGGTTTAACTGAGAATATCGTTAGAGAAATTTCTAACATG
  AAAAATGAGCCGGAATGGATGTTAGATTTCCGTCTTAAATCATTAAAATTGTTTTATAAA
  ATGCCAATGCCTCAATGGGGTGGCGACTTATCAGAATTGAATTTCGATGACATTACTTAC
  TATGTAAAGCCTTCAGAACAAGCTGAACGTTCATGGGATGAAGTGCCAGAAGAAATTAAA
  AGAACTTTCGATAAATTAGGAATTCCTGAAGCTGAACAAAAATATTTAGCTGGTGTTTCT
  GCTCAATATGAATCTGAAGTTGTTTACCATAATATGGAAAAAGAACTTGAAGAAAAAGGT
  ATTATCTTTAAAGATACAGATAGTGCTTTACAAGAAAATGAAGAATTATTCAAAAAATAC
  TTTGCTTCTGTAGTACCTGCAGCAGATAACAAATTTGCGGCGTTAAACTCAGCAGTATGG
  TCAGGTGGTTCGTTCATTTATGTACCTAAAAATATCAAACTAGATACGCCACTACAAGCT
  TATTTCCGTATTAACTCTGAGAACATGGGTCAATTTGAACGTACATTAATCATTGCTGAT
  GAAGGTGCTTCTGTACATTACGTAGAAGGTTGTACTGCACCAGTTTATACAACTAGTTCT
  TTACACTCTGCTGTTGTGGAAATCATTGTGCATAAAGATGCGCACGTTCGTTATACTACG
  ATTCAAAACTGGGCGAACAATGTATACAATTTAGTTACAAAACGTACTTTTGTTTATGAA
  AACGGAAATATGGAATGGGTAGATGGTAACTTAGGTTCTAAGTTAACGATGAAATATCCA
  AACTGTGTTCTTTTAGGTGAAGGTGCAAAAGGTAGTACATTATCTATTGCATTTGCTGGT
  AAAGGACAAGTTCAAGATGCCGGTGCTAAAATGATTCATAAAGCACCTAATACATCTTCT
  ACAATTGTTTCTAAATCTATTTCTAAAAATGGTGGTAAAGTTATTTATCGCGGTATTGTT
  CATTTTGGACGTAAAGCAAAAGGTGCTCGTTCAAATATTGAATGTGATACATTAATCTTA
  GATAACGAATCAACATCAGATACAATTCCATATAACGAAGTATTCAACGATCAAATATCA
  TTAGAACATGAAGCCAAGGTTTCAAAAGTTTCTGAAGAACAATTATTCTATCTAATGAGT
  CGTGGTATTTCTGAAGAAGAAGCGACAGAAATGATTGTTATGGGATTCATCGAACCATTT
  ACAAAAGAACTTCCAATGGAATACGCGGTCGAAATGAACCGTTTAATCAAGTTCGAAATG
  GAAGGTAGTATTGGATAG
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⊟Protein

⊟General

• locus tag: NWMN_0789 [new locus tag: NWMN_RS04460 ]
• symbol: SufB
• description: FeS assembly protein SufB
• length: 465
• theoretical pI: 4.81321
• theoretical MW: 52531.1
• GRAVY: -0.397849

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufB (TIGR01980; HMM-score: 767.9)
  and 1 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufD (TIGR01981; HMM-score: 201.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined UPF0051; Uncharacterized protein family (UPF0051) (PF01458; HMM-score: 250.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003263
  • TAT(Tat/SPI): 0.000265
  • LIPO(Sec/SPII): 0.000357
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221001 NCBI
• RefSeq: YP_001331823 NCBI
• UniProt: A0A0H3KBP4 UniProt

⊟Protein sequence

• MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEPEWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKYFASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLIIADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRTFVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHKAPNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYNEVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sufC > sufD > sufS > nifU > sufB

⊟Regulation

• regulator: PerR* (repression) regulon

  PerR*

  (TF)

  important in Oxidative stress response; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications