PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
• pan locus tag^?: SAUPAN004758000
• symbol: hemE
• pan gene symbol^?: hemE
• synonym:
• product: uroporphyrinogen decarboxylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
• symbol: hemE
• product: uroporphyrinogen decarboxylase
• replicon: chromosome
• strand: -
• coordinates: 1925657..1926694
• length: 1038
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331990 NCBI
• RefSeq: YP_001332759 NCBI
• BioCyc:
• MicrobesOnline: 3707285 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGTGCATAATAAAAACAATACAATTTTAAAAATGATCAAAGGTGAAGAAACATCACAT
  ACACCTGTTTGGTTTATGCGACAAGCTGGCCGTTCGCAACCAGAATATCGAAAATTGAAA
  GAAAAATATTCACTATTCGATATTACACATCAGCCGGAGTTGTGCGCTTATGTAACACAT
  TTACCAGTTGATAATTATCATACAGATGCAGCAATTTTATACAAAGATATTATGACACCA
  TTAAAGCCAATTGGTGTCGATGTAGAAATTAAATCGGGTATTGGTCCAGTGATTCATAAT
  CCAATCAAAACAATTCAAGATGTTGAGAAACTTTCTCAAATAGACCCCGAACGAGATGTA
  CCATATGTATTAGATACAATTAAACTTTTAACAGAAGAAAAGTTAAATGTGCCGCTAATA
  GGATTTACTGGGGCACCATTTACATTAGCGTCATATATGATTGAAGGCGGACCATCGAAA
  AATTACAATTTTACAAAAGCGATGATGTATAGAGATGAAGCAACATGGTTTGCTTTAATG
  AATCATTTAGTTGATGTATCTGTTAAATATGTAACAGCTCAAGTCGAAGCAGGTGCCGAA
  TTGATTCAAATTTTCGATTCATGGGTAGGTGCATTAAATGTCGAGGATTACAGACGTTAC
  ATTAAACCACATATGATTCGATTAATCAGTGAGGTTAAAGAAAAACATGATGTGCCGGTA
  ATTTTATTCGGTGTAGGTGCCAGTCATTTAATCAATGAATGGAATGATTTACCGATTGAT
  GTATTAGGCCTAGATTGGAGAACGTCTATTAATCAGGCTCAACAATTAGGCGTTACTAAA
  ACATTACAAGGGAATTTAGATCCATCAATTTTATTAGCACCATGGAATGTCATTGAAGAG
  AGATTGAAACCAATATTAGATCAAGGTATGGAGAACGGTAAACACATCTTTAATTTAGGA
  CACGGTGTATTCCCAGAAGTGCAACCAGAGACGTTACGTAAAGTAAGCGAATTCGTACAC
  ACATATACACAAAGATAA
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⊟Protein

⊟General

• locus tag: NWMN_1725 [new locus tag: NWMN_RS09725 ]
• symbol: HemE
• description: uroporphyrinogen decarboxylase
• length: 345
• theoretical pI: 6.324
• theoretical MW: 39352.1
• GRAVY: -0.231304

⊟Function

• reaction:
  EC 4.1.1.37?  ExPASy
  Uroporphyrinogen decarboxylase Uroporphyrinogen III = coproporphyrinogen + 4 CO₂
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen decarboxylase (TIGR01464; EC 4.1.1.37; HMM-score: 420.6)
  and 2 more
  methyltransferase, MtaA/CmuA family (TIGR01463; EC 2.1.1.-; HMM-score: 61.8)
  Signal transduction Regulatory functions Protein interactions phosphohistidine phosphatase SixA (TIGR00249; EC 3.1.3.-; HMM-score: 15.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Methionine_synt (CL0160) URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 403.6)
  and 1 more
  TPR (CL0020) COPI_C; Coatomer (COPI) alpha subunit C-terminus (PF06957; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006385
  • TAT(Tat/SPI): 0.000484
  • LIPO(Sec/SPII): 0.000852
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221937 NCBI
• RefSeq: YP_001332759 NCBI
• UniProt: A6QI15 UniProt

⊟Protein sequence

• MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYSLFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPIKTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSKNYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYRRYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQLGVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRKVSEFVHTYTQR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hemY < hemH < hemE

⊟Regulation

• regulator: PerR* (repression) regulon

  PerR*

  (TF)

  important in Oxidative stress response; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications