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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1184 [new locus tag: NWMN_RS06675 ]
- pan locus tag?: SAUPAN003576000
- symbol: NWMN_1184
- pan gene symbol?: rnjB
- synonym:
- product: RNA-metabolising metallo-beta-lactamase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1184 [new locus tag: NWMN_RS06675 ]
- symbol: NWMN_1184
- product: RNA-metabolising metallo-beta-lactamase
- replicon: chromosome
- strand: +
- coordinates: 1304379..1306052
- length: 1674
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332277 NCBI
- RefSeq: YP_001332218 NCBI
- BioCyc:
- MicrobesOnline: 3706736 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621TTGAGTTTAATAAAGAAAAAGAATAAAGATATTCGCATTATACCATTAGGCGGTGTTGGC
GAAATTGCTAAAAATATGTATATCGTTGAAGTAGACGATGAAATGTTTATGTTAGATGCT
GGACTTATGTTTCCAGAAGACGAAATGCTAGGTATTGATATTGTTATACCAGACATTTCA
TACGTACTTGAAAATAAAGATAAATTGAAGGGTATATTCCTTACACACGGACATGAGCAC
GCGATTGGTGCAGTGAGTTATGTTTTAGAACAATTAGATGCACCAGTATATGGATCTAAA
TTGACAATAGCGTTAATTAAAGAAAATATGAAAGCCCGTAATATTGATAAAAAAGTTCGC
TACTATACAGTTAATAATGATTCAATTATGAGATTCAAAAACGTGAATATTAGTTTCTTT
AATACGACACACAGTATTCCTGATAGTTTAGGTGTTTGTATTCACACTTCATATGGTGCC
ATTGTGTATACAGGTGAATTTAAGTTTGACCAAAGTTTACATGGACATTATGCACCAGAT
ATTAAACGTATGGCAGAGATTGGTGAAGAAGGCGTATTTGTCTTAATCAGTGATTCTACT
GAGGCAGAGAAACCTGGATATAATACTCCGGAAAATGTGATTGAACATCATATGTATGAT
GCTTTTGCAAAAGTGCGAGGTCGCTTGATAGTTTCATGTTATGCTTCGAACTTTATACGT
ATTCAGCAAGTTTTAAATATTGCTAGCAAGCTAAATCGTAAAGTGTCATTTTTAGGAAGA
TCACTTGAAAGTTCATTTAATATTGCTCGTAAAATGGGGTATTTCGACATTCCTAAAGAT
TTGCTAATTCCTATAACAGAAGTTGATAATTATCCTAAAAATGAAGTGATAATTATAGCT
ACTGGTATGCAAGGAGAACCTGTAGAAGCCTTAAGTCAAATGGCGCAACATAAGCATAAA
ATTATGAATATCGAAGAAGGCGATTCTGTATTTTTAGCAATTACGGCTTCTGCTAATATG
GAAGTTATCATTGCGAATACATTAAATGAGCTTGTACGTGCTGGCGCACATATTATTCCA
AATAACAAAAAGATTCATGCTTCAAGTCATGGTTGCATGGAAGAATTAAAAATGATGATT
AATATTATGAAACCTGAATACTTTATTCCTGTACAAGGTGAATTTAAAATGCAGATAGCA
CATGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAA
GGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGA
AATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGT
CATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGA
CGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAA
GACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTACAAGAA
AAACGCATAGAATGGTCTGAAATTAAACAAAATATGCGTGATCAAATTAGTAAACTATTA
TTCGAAAGTACAAAACGTCGTCCTATGATTATTCCAGTAATTTCTGAAATTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1184 [new locus tag: NWMN_RS06675 ]
- symbol: NWMN_1184
- description: RNA-metabolising metallo-beta-lactamase
- length: 557
- theoretical pI: 6.10952
- theoretical MW: 62603.1
- GRAVY: -0.0983842
⊟Function[edit | edit source]
- reaction: EC 3.1.-.-? ExPASy
- TIGRFAM: Transcription Degradation of RNA beta-CASP ribonuclease, RNase J family (TIGR00649; EC 3.1.-.-; HMM-score: 606)and 2 morearCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 26.3)Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 16.9)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: RMMBL_DRMBL (CL0398) RMMBL; Zn-dependent metallo-hydrolase RNA specificity domain (PF07521; HMM-score: 44.4)and 4 moreMetallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 29.7)Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 22.6)ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.1)HTH (CL0123) HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001198
- TAT(Tat/SPI): 0.000054
- LIPO(Sec/SPII): 0.000176
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMFPEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_1605 (ackA) acetate kinase [1] (data from MRSA252) NWMN_0577 (adh1) alcohol dehydrogenase [1] (data from MRSA252) NWMN_0372 (ahpC) alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) NWMN_2534 (arcA) arginine deiminase [1] (data from MRSA252) NWMN_2533 (arcB) ornithine carbamoyltransferase [1] (data from MRSA252) NWMN_1365 (asnC) asparaginyl-tRNA synthetase [1] (data from MRSA252) NWMN_0736 (clpP) ATP-dependent Clp protease proteolytic subunit [1] (data from MRSA252) NWMN_2042 (deoD) purine nucleoside phosphorylase [1] (data from MRSA252) NWMN_1483 (dnaK) molecular chaperone DnaK [1] (data from MRSA252) NWMN_0002 (dnaN) DNA polymerase III subunit beta [1] (data from MRSA252) NWMN_0745 (eno) phosphopyruvate hydratase [1] (data from MRSA252) NWMN_0551 (eutD) phosphotransacetylase [1] (data from MRSA252) NWMN_1625 (fhs) formate--tetrahydrofolate ligase [1] (data from MRSA252) NWMN_1096 (ftsZ) cell division protein FtsZ [1] (data from MRSA252) NWMN_0509 (fus) elongation factor G [1] (data from MRSA252) NWMN_0741 (gapA) glyceraldehyde 3-phosphate dehydrogenase 1 [1] (data from MRSA252) NWMN_2056 (glmS) glucosamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) NWMN_1217 (glnA) glutamine synthetase [1] (data from MRSA252) NWMN_1468 (glyS) glycyl-tRNA synthetase [1] (data from MRSA252) NWMN_1417 (gnd) 6-phosphogluconate dehydrogenase [1] (data from MRSA252) NWMN_2315 (gpmA) phosphoglyceromutase [1] (data from MRSA252) NWMN_0380 (guaB) inosine-5'-monophosphate dehydrogenase [1] (data from MRSA252) NWMN_0828 (gudB) NAD-specific glutamate dehydrogenase [1] (data from MRSA252) NWMN_0516 (ilvE) branched-chain amino acid aminotransferase [1] (data from MRSA252) NWMN_1178 (infB) translation initiation factor IF-2 [1] (data from MRSA252) NWMN_2499 (ldh) L-lactate dehydrogenase 2 [1] (data from MRSA252) NWMN_0176 (ldh1) L-lactate dehydrogenase [1] (data from MRSA252) NWMN_2028 (murZ) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252) NWMN_2446 (mvaS) 3-hydroxy-3-methylglutaryl coenzyme A synthase [1] (data from MRSA252) NWMN_0962 (pdhD) dihydrolipoamide dehydrogenase [1] (data from MRSA252) NWMN_2040 (pdp) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) NWMN_1593 (pfk) 6-phosphofructokinase [1] (data from MRSA252) NWMN_0162 (pflB) formate acetyltransferase [1] (data from MRSA252) NWMN_0833 (pgi) glucose-6-phosphate isomerase [1] (data from MRSA252) NWMN_0742 (pgk) phosphoglycerate kinase [1] (data from MRSA252) NWMN_0744 (pgm) phosphoglyceromutase [1] (data from MRSA252) NWMN_0959 (phdA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) NWMN_2438 (poxB) pyruvate oxidase [1] (data from MRSA252) NWMN_1592 (pykA) pyruvate kinase [1] (data from MRSA252) NWMN_2031 (pyrG) CTP synthetase [1] (data from MRSA252) NWMN_0500 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) NWMN_2149 (rplB) 50S ribosomal protein L2 [1] (data from MRSA252) NWMN_2151 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) NWMN_2140 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) NWMN_2137 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) NWMN_0014 (rplI) 50S ribosomal protein L9 [1] (data from MRSA252) NWMN_0501 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) NWMN_0499 (rplK) 50S ribosomal protein L11 [1] (data from MRSA252) NWMN_0502 (rplL) 50S ribosomal protein L7/L12 [1] (data from MRSA252) NWMN_2120 (rplM) 50S ribosomal protein L13 [1] (data from MRSA252) NWMN_2133 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) NWMN_2125 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) NWMN_1151 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_1572 (rplT) 50S ribosomal protein L20 [1] (data from MRSA252) NWMN_1549 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) NWMN_2147 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_2150 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) NWMN_2141 (rplX) 50S ribosomal protein L24 [1] (data from MRSA252) NWMN_0464 (rplY) 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) NWMN_2024 (rpmE2) 50S ribosomal protein L31 type B [1] (data from MRSA252) NWMN_1573 (rpmI) 50S ribosomal protein L35 [1] (data from MRSA252) NWMN_2126 (rpoA) DNA-directed RNA polymerase subunit alpha [1] (data from MRSA252) NWMN_0505 (rpoC) DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) NWMN_1166 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) NWMN_2146 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) NWMN_1613 (rpsD) 30S ribosomal protein S4 [1] (data from MRSA252) NWMN_2135 (rpsE) 30S ribosomal protein S5 [1] (data from MRSA252) NWMN_0357 (rpsF) 30S ribosomal protein S6 [1] (data from MRSA252) NWMN_0508 (rpsG) 30S ribosomal protein S7 [1] (data from MRSA252) NWMN_2138 (rpsH) 30S ribosomal protein S8 [1] (data from MRSA252) NWMN_2119 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) NWMN_2153 (rpsJ) 30S ribosomal protein S10 [1] (data from MRSA252) NWMN_2127 (rpsK) 30S ribosomal protein S11 [1] (data from MRSA252) NWMN_0507 (rpsL) 30S ribosomal protein S12 [1] (data from MRSA252) NWMN_2128 (rpsM) 30S ribosomal protein S13 [1] (data from MRSA252) NWMN_1148 (rpsP) 30S ribosomal protein S16 [1] (data from MRSA252) NWMN_2143 (rpsQ) 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_2148 (rpsS) 30S ribosomal protein S19 [1] (data from MRSA252) NWMN_0008 (serS) seryl-tRNA synthetase [1] (data from MRSA252) NWMN_1456 (sodA) superoxide dismutase Mn/Fe family protein [1] (data from MRSA252) NWMN_0055 (spa) immunoglobulin G binding protein A precursor (protein A) [1] (data from MRSA252) NWMN_1325 (sucB) dihydrolipoamide succinyltransferase [1] (data from MRSA252) NWMN_1155 (sucC) succinyl-CoA synthetase subunit beta [1] (data from MRSA252) NWMN_1156 (sucD) succinyl-CoA synthetase subunit alpha [1] (data from MRSA252) NWMN_1576 (thrS) threonyl-tRNA synthetase [1] (data from MRSA252) NWMN_1569 (tig) trigger factor [1] (data from MRSA252) NWMN_1254 (tkt) transketolase [1] (data from MRSA252) NWMN_0743 (tpiA) triosephosphate isomerase [1] (data from MRSA252) NWMN_1057 (trxA) thioredoxin [1] (data from MRSA252) NWMN_1167 (tsf) elongation factor Ts [1] (data from MRSA252) NWMN_0510 (tufA) elongation factor Tu [1] (data from MRSA252) NWMN_2016 (upp) uracil phosphoribosyltransferase [1] (data from MRSA252) NWMN_0428 ABC transporter substrate-binding protein [1] (data from MRSA252) NWMN_0475 cysteine synthase-like protein [1] (data from MRSA252) NWMN_0481 pyridoxal biosynthesis lyase PdxS [1] (data from MRSA252) NWMN_0601 hypothetical protein [1] (data from MRSA252) NWMN_0655 MarR family regulatory protein [1] (data from MRSA252) NWMN_0721 sigma 54 modulation protein [1] (data from MRSA252) NWMN_0774 hypothetical protein [1] (data from MRSA252) NWMN_0776 glycine cleavage system protein H [1] (data from MRSA252) NWMN_0811 hypothetical protein [1] (data from MRSA252) NWMN_0870 oligoendopeptidase F [1] (data from MRSA252) NWMN_0949 phosphocarrier protein HPr [1] (data from MRSA252) NWMN_0950 phosphoenolpyruvate-protein phosphatase [1] (data from MRSA252) NWMN_1263 aconitate hydratase [1] (data from MRSA252) NWMN_1382 DNA-binding protein HU [1] (data from MRSA252) NWMN_1600 universal stress protein family protein [1] (data from MRSA252) NWMN_1602 proline dipeptidase-like protein [1] (data from MRSA252) NWMN_1604 universal stress protein family protein [1] (data from MRSA252) NWMN_1643 D-alanine aminotransferase [1] (data from MRSA252) NWMN_1672 putative translaldolase [1] (data from MRSA252) NWMN_2086 alkaline shock protein 23 [1] (data from MRSA252) NWMN_2422 D-lactate dehydrogenase [1] (data from MRSA252) NWMN_2454 1-pyrroline-5-carboxylate dehydrogenase [1] (data from MRSA252) NWMN_2503 fructose-1,6-bisphosphate aldolase [1] (data from MRSA252) NWMN_2504 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)