PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
• pan locus tag^?: SAUPAN001695000
• symbol: NWMN_1028
• pan gene symbol^?: —
• synonym:
• product: phage tape measure protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
• symbol: NWMN_1028
• product: phage tape measure protein
• replicon: chromosome
• strand: +
• coordinates: 1124831..1128295
• length: 3465
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330632 NCBI
• RefSeq: YP_001332062 NCBI
• BioCyc:
• MicrobesOnline: 3706579 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGACGGAATATAAAATTAAAGCGACTATTGAAGCTAGTGTAGCCAAATTCAAAAGGCAA
  ATCGATAGTGCGGTTAAGTCTGTGCAAAGATTTAAACGAGTAGCAGATCAAACTAAAGAT
  GTCGAATTAAATGCTGATGATAAAAAATTACAAAAAACTATCAAAGTTGCTAAAAAGTCT
  TTAGATGCCTTTAGTAACAAAAAAGTAAAAGCTAAATTAGATGCTAGTATACAAGACTTG
  CAACAAAAGGTACTAGAATCGAATTTTGAACTAGACAAACTAAACTCTAAAGAAGTTACT
  CCAGAGATTAAATTACAAAAACAAAAATTGACTAAAGATATCGCTGAAGCAGAAGCTAAG
  TTATCCGAACTAGAAAAGAAGCGTGTCAATATTGACATCAATGCAGATAACAGTAAATTC
  AATCGAGTGTTAAAAGTATCTAAAGCTAGTCTTGAAGCATTAAATAGGTCTAAAGCCAAA
  GCTATTATAGACGTGGACAATGGTGTTGCTAACTCTAAAATAAAACGCACTAAAGAAGAA
  CTTAAAAGTATTCCAAACAAAACTAGATCTCGACTAGATGTAGATACAGGACTTTCTATA
  CCAACTATTTATGCGTTTAAAAAATCATTAGACGCATTGCCAAACAAAAAAACAACGAAG
  GTAGATGTCGATACTAACGGTTTAAAGAAAGCTTATGCCTACATAATAAAAGTAAACGAC
  AATTTCCAAAGACAAATGGGGAATTTAGCTAATATGTTTCGCGTGTTCGGCACTGTAGGT
  TCTAATATGGTTGGTGGATTACTTACATCATCTTTTAGTATCTTAATACCTGTAATAGCG
  AGCGTAGTACCTGTAGTATTTGCGCTATTAAACGCTATCAAAGTGTTAACTGGCGGTGTA
  CTTGCTTTAGGTGGTGCTGTAGCAATAGCTGGTGCTGGCTTTGTAGCATTTGGCGCAATG
  GCTATCAGCGCTATAAAGATGCTTAGTGACGGCACTTTACAAGCTAGCTCAGCAACAAAC
  GAATACAAAAAAGCTTTAGATGGCGTAAAGTCAGCATGGACTGATATTATAAAGCAAAAT
  CAATCCGCTATCTTCACAACTCTTGCAAATGGTTTAAATACTGTTAAAACAGCAATGCAG
  AGCTTACAACCGTTTTTTAGTGGTATTTCAAGAGGAATGGAAGAGGCGTCTCAAAGTGTA
  CTTAAATGGGCTCAAAATAGCGGTGTAGCATCAAGGTTCTTCAACATGATGAATACAACT
  GGTGTTTCGGTATTTAACAAGCTATTAAGTGCTGCAGGAGGTTTCGGTGACGGATTAGTC
  AATGTGTTCACACAATTAGCACCACTGTTTCAATGGTCGGCTGATTGGTTGGATAGATTA
  GGTCAATCATTCTCTAATTGGGCTAATAGTGCAGCTGGAGAAAATTCGATAACTCGTTTT
  ATTGAATACACAAAAACAAACTTACCTATCATTGGTAATATTTTTAAAAATGTTTTCGTT
  GGAATTAACAATTTGATGAATGCATTCAGTGGATCATCAACTGGCATATTCCAATCTCTT
  GAACAAATGACAGCTAAGTTTAGGGAATGGTCTGAACAAGTAGGACAATCTCAAGGGTTT
  AAAGATTTTGTCAGCTATATACAAACTAATGGACCACTAATAATGCAATTAATTGGGAAC
  ATTGCAAGAGGATTAGTTGCATTCGCAACAGCGATGGCTCCTATAGCTAGTGCAGTATTA
  CGCGTTGCAGTAGCAATAACTGGTTGGATAGCTAACTTGTTTGAGGCGCATCCAGCTACA
  GCACAATTAGTTGGCGTCATTATAACTTTAGTTGGTGCATTTAGATTTTTAATTGCTCCA
  ATATTAGCGGTAATGGATTTTCTGGGGCCATTAGCAGCAAGATTAGTCGCATTAGTAACT
  AAGTTTGGTTGGGCTAAAACAGGAACTTTAGTATTAAGTAAGGCAATGACATCATTAAAA
  GGTCCAATAAAATTAGTTACAGCTATATTCCAATTGTTATTCGGTAAGATTGGATTAATT
  AGAAATGCTATCACAGGACTAGTAACTGTGTTTGGTATTTTAGGTGGTCCAATAACAATA
  GTAATTGGTGTAATCGCTGCATTAATAGCTATATTTGTTTTATTGTGGAATAAAAATGAA
  GGATTCAGAAACTTTATTATAAATGTTTGGAATGCGATAAAAACATTTATGGTTACAGTT
  TGGAATGTGTTGAAAACTGTAGCTTCGGTTGTATGGAATGCTATTTTAAAAGCTATCACT
  ACAGCAGTATCAAATGTATACAATTTTATAATGATTATTTGGAATCAAATAGTTGCTTAT
  TTGCAAGGGCTATGGAATGGAATTATCGCTATTGCAACAACAGTATGGAACCTTTTAGTT
  ACAATCATCACAACTGTTTTCACGACGATAATGACAATAGTTATGACGATATGGACAGCT
  ATTTGGACATTCTTAAGTACAATCTGGAACACGATAATTACAATCGCTACTACGATTTGG
  AATTTGTTAGTCACTGTAATAACTACAGTATTTACCACAATTATGACTATCGCAATGACA
  ATTTGGAACGCTATTTGGACGTTCTTACAAACGTTGTGGAACACTATAGTTACTGTGGCA
  ACTAAGGTTTGGAACGCTATCACTACAACTATATCTACTGCGTTACAAGCGGCATGGAGT
  TTTATTTCTAATATATGGAATACGATTTGGAGTTTCTTATCTGGTATATTAACGACAATT
  TGGAATAAAGTTGTAAGCATATTCACACAAGTTGTATCAACTATATCAGACAAAATGTCT
  CAAGCTTGGAACTTCATCGTGACTAAAGGTATGCAATGGGTATCTACTATAACAAGTACG
  CTAATTAACTTTGTTAATAGAGTTATTCAAGGATTCGTTAATGTTGTAAACAAAGTTAGT
  CAAGGTATGACAAATGCAGTAAATAAAATAAAAAGTTTTATAGGAGATTTTGTGTCTGCA
  GGTGCTGATATGATCCGTGGTTTAATTAGAGGTATTGGACAAATGGCTGGTCAATTAGTA
  GATGCAGCTAAAAATGTTGCTAAGAAAGCTTTAGATGCAGCTAAAAGTGCTTTGGGTATT
  CACTCACCTTCACGTGAATTCATGGATGTTGGTATGTATTCAATGCTAGGTTTCGTTAAA
  GGTATAGATAATCATTCAAGTAAAGTTATCCGTAATGTTTCTAATGTTGCAGATAAAGTA
  GTTGATGCATTTCAACCTACATTAAACGCACCTGACATTTCTAGTATTACAGGAAACTTA
  AGTAATTTAGGTGGAAATATAAATGCGCAAGTACAACACACACATTCTATTGAAACATCA
  CCGAACATGAAAACTGTTAAAGTTGAATTCGATGTTAATAACGATGCGCTTACTAGTATT
  GTTAACGGCAGAAATGCTAAACGCAATTCTGAGTATTACTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_1028 [new locus tag: NWMN_RS05820 ]
• symbol: NWMN_1028
• description: phage tape measure protein
• length: 1154
• theoretical pI: 10.6502
• theoretical MW: 125759
• GRAVY: 0.314905

⊟Function

• TIGRFAM:
• TheSEED: data available for NCTC8325
• PFAM:
  HTH (CL0123) DUF2316; Uncharacterized protein conserved in bacteria (DUF2316) (PF10078; HMM-score: 10.2)
  and 2 more
  Traffic (CL0147) Sec20; Sec20 (PF03908; HMM-score: 8)
  no clan defined Phasin_2; Phasin protein (PF09361; HMM-score: 7.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006002
  • TAT(Tat/SPI): 0.000595
  • LIPO(Sec/SPII): 0.001263
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 151221240 NCBI
• RefSeq: YP_001332062 NCBI
• UniProt: A0A0H3K7J1 UniProt

⊟Protein sequence

• MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELNADDKKLQKTIKVAKKSLDAFSNKKVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPEIKLQKQKLTKDIAEAEAKLSELEKKRVNIDINADNSKFNRVLKVSKASLEALNRSKAKAIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKTTKVDVDTNGLKKAYAYIIKVNDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILIPVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLSDGTLQASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRGMEEASQSVLKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLFQWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNAFSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLVAFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIAPILAVMDFLGPLAARLVALVTKFGWAKTGTLVLSKAMTSLKGPIKLVTAIFQLLFGKIGLIRNAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINVWNAIKTFMVTVWNVLKTVASVVWNAILKAITTAVSNVYNFIMIIWNQIVAYLQGLWNGIIAIATTVWNLLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIMTIAMTIWNAIWTFLQTLWNTIVTVATKVWNAITTTISTALQAAWSFISNIWNTIWSFLSGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQGFVNVVNKVSQGMTNAVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKKALDAAKSALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPTLNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRNAKRNSEYYL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1018 > NWMN_1019 > NWMN_1020 > NWMN_1021 > NWMN_1022 > NWMN_1023 > NWMN_1024 > NWMN_1025 > NWMN_1026 > NWMN_1027 > NWMN_1028 > NWMN_1029 > NWMN_1030 > NWMN_1031 > NWMN_1032 > NWMN_1033

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications