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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0726 [new locus tag: NWMN_RS04115 ]
  • pan locus tag?: SAUPAN002672000
  • symbol: uvrB
  • pan gene symbol?: uvrB
  • synonym:
  • product: excinuclease ABC subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0726 [new locus tag: NWMN_RS04115 ]
  • symbol: uvrB
  • product: excinuclease ABC subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 814435..816420
  • length: 1986
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGTTGAACATTATCCTTTTAAAATACATTCTGATTTTGAGCCTCAAGGTGACCAACCG
    CAAGCAATTAAAGAAATCGTGGAAGGTATTAAAGCGGGGAAAAGACATCAAACTTTATTA
    GGTGCTACTGGCACAGGGAAAACATTTACGATGAGTAATGTTATTAAAGAAGTTGGGAAA
    CCAACGTTAATTATCGCGCATAACAAAACATTAGCAGGACAATTATATAGTGAGTTTAAA
    GAATTTTTTCCTGAAAACAGGGTGGAATACTTTGTAAGTTACTATGATTATTATCAACCA
    GAGGCATACGTACCGTCTACTGACGCTTTTATGGAAAAAGATGCCTCAATCAATGATGAA
    ATTGATCAAGTACGACATTCTGCTACAAGTGCATTATTTGAACGCGATGATGTAATTATT
    ATTGCTAGTGTAAGTTGTATATATGGTTTAGGTAATCCTGAAGAATATAAAGATTTAGTA
    GTAAGTGTTCGAGTTGGTATGGAAATGGATAGAAGTGAATTACTTAGAAAACTTGTAGAT
    GTGCAATATACACGAAATGACATCGATTTCCAACGAGGAACGTTTCGAGTGCGTGGTGAT
    GTAGTGGAAATATTCCCAGCCTCTAAAGAAGAACTTTGTATAAGGGTTGAGTTTTTCGGC
    GATGAGATTGACCGTATCCGAGAAGTTAACTACCTAACAGGTGAAGTGTTGAAAGAAAGA
    GAACATTTTGCGATATTCCCAGCTTCTCACTTCGTAACACGTGAAGAAAAGTTGAAAGTT
    GCGATTGAACGTATTGAAAAAGAATTGGAAGAACGATTGAAAGAATTACGAGATGAGAAT
    AAATTACTAGAAGCGCAAAGGTTAGAACAGCGTACCAACTATGATTTAGAAATGATGCGA
    GAGATGGGATTCTGTTCAGGAATTGAAAACTATTCCGTACATTTAACTTTGCGACCACTG
    GGTTCGACACCATATACTTTATTGGATTACTTTGGCGATGATTGGTTAGTAATGATTGAT
    GAATCACATGTGACATTACCGCAAGTTCGAGGCATGTATAACGGAGACAGAGCGCGTAAA
    CAAGTTTTGGTGGATCATGGGTTTAGATTACCGAGTGCATTAGATAACCGTCCACTTAAA
    TTTGAAGAATTTGAAGAAAAGACAAAACAACTTGTGTATGTATCTGCAACGCCTGGACCA
    TACGAAATTGAACATACGGATAAGATGGTTGAACAAATTATTCGTCCTACTGGTTTACTG
    GATCCTAAGATTGAGGTTAGACCTACTGAAAATCAAATTGACGATTTATTAAGTGAAATT
    CAAACAAGAGTTGAGCGTAATGAACGCGTACTTGTTACAACGCTCACTAAAAAGATGAGT
    GAAGATTTAACCACATACATGAAAGAAGCGGGTATTAAAGTTAATTATCTGCATTCAGAA
    ATCAAGACATTAGAACGAATTGAAATAATTAGAGACTTACGAATGGGTACATATGATGTT
    ATCGTAGGTATTAATTTATTAAGAGAGGGTATTGATATACCAGAAGTTTCTCTAGTTGTC
    ATATTAGATGCAGATAAAGAAGGGTTTTTACGTTCTAACCGCTCATTAATTCAAACAATA
    GGTAGAGCTGCGCGTAACGATAAAGGTGAAGTCATTATGTATGCCGATAAAATGACTGAT
    TCGATGAAGTATGCAATTGATGAGACACAACGTCGTCGAGAAATACAGATGAAACATAAT
    GAAAAACATGGTATTACACCTAAAACAATTAATAAAAAAATACATGATTTAATTAGTGCT
    ACTGTTGAAAATGACGAAAATAATGACAAAGCACAAACTGTGATACCTAAGAAGATGACG
    AAAAAAGAACGTCAAAAGACAATCGACAATATAGAAAAAGAAATGAAACAAGCAGCGAAA
    GATTTAGATTTCGAGAAAGCTACAGAATTAAGAGATATGTTATTTGAATTAAAAGCAGAA
    GGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1986

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0726 [new locus tag: NWMN_RS04115 ]
  • symbol: UvrB
  • description: excinuclease ABC subunit B
  • length: 661
  • theoretical pI: 5.05392
  • theoretical MW: 76621.8
  • GRAVY: -0.55416

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 1104.8)
    and 8 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 65.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 47.4)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 40)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 32.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 27.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 27.6)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 18.1)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 13.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) UvrB; Ultra-violet resistance protein B (PF12344; HMM-score: 84.2)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 73.4)
    and 14 more
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 46.3)
    no clan defined UVR; UvrB/uvrC motif (PF02151; HMM-score: 35.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 23.7)
    AAA_30; AAA domain (PF13604; HMM-score: 22.1)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 15.9)
    Kinesin; Kinesin motor domain (PF00225; HMM-score: 13.6)
    MEDS; MEDS: MEthanogen/methylotroph, DcmR Sensory domain (PF14417; HMM-score: 12.7)
    DUF853; Bacterial protein of unknown function (DUF853) (PF05872; HMM-score: 12.4)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 12.3)
    AAA_19; AAA domain (PF13245; HMM-score: 11.2)
    AAA_25; AAA domain (PF13481; HMM-score: 11.2)
    PDDEXK (CL0236) Sen15; Sen15 protein (PF09631; HMM-score: 10.8)
    Oxa1 (CL0376) DUF106; Integral membrane protein DUF106 (PF01956; HMM-score: 9.9)
    P-loop_NTPase (CL0023) DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02003
    • TAT(Tat/SPI): 0.022381
    • LIPO(Sec/SPII): 0.000795
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATGTGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEAYVPSTDAFMEKDASINDEIDQVRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLVVSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEFFGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKELRDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDDWLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLVYVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERVLVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLREGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAIDETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKERQKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1605(ackA)acetate kinase  [1] (data from MRSA252)
    NWMN_0372(ahpC)alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_1483(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    NWMN_0745(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    NWMN_0551(eutD)phosphotransacetylase  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_2056(glmS)glucosamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    NWMN_1217(glnA)glutamine synthetase  [1] (data from MRSA252)
    NWMN_0380(guaB)inosine-5'-monophosphate dehydrogenase  [1] (data from MRSA252)
    NWMN_1178(infB)translation initiation factor IF-2  [1] (data from MRSA252)
    NWMN_0176(ldh1)L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_2028(murZ)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0162(pflB)formate acetyltransferase  [1] (data from MRSA252)
    NWMN_0744(pgm)phosphoglyceromutase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2120(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2126(rpoA)DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    NWMN_0505(rpoC)DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    NWMN_2032(rpoE)DNA-directed RNA polymerase subunit delta  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0508(rpsG)30S ribosomal protein S7  [1] (data from MRSA252)
    NWMN_2128(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_0359(rpsR)30S ribosomal protein S18  [1] (data from MRSA252)
    NWMN_2148(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    NWMN_1456(sodA)superoxide dismutase Mn/Fe family protein  [1] (data from MRSA252)
    NWMN_0055(spa)immunoglobulin G binding protein A precursor (protein A)  [1] (data from MRSA252)
    NWMN_1326(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_1569(tig)trigger factor  [1] (data from MRSA252)
    NWMN_0743(tpiA)triosephosphate isomerase  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0727(uvrA)excinuclease ABC subunit A  [1] (data from MRSA252)
    NWMN_1058(uvrC)excinuclease ABC subunit C  [1] (data from MRSA252)
    NWMN_0443hypothetical protein  [1] (data from MRSA252)
    NWMN_0475cysteine synthase-like protein  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0839fumarylacetoacetate hydrolase family protein  [1] (data from MRSA252)
    NWMN_0949phosphocarrier protein HPr  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_1643D-alanine aminotransferase  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: LexA (repression) regulon
    LexA(TF)important in SOS response; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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