PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1058 [new locus tag: NWMN_RS05980 ]
• pan locus tag^?: SAUPAN003385000
• symbol: uvrC
• pan gene symbol^?: uvrC
• synonym:
• product: excinuclease ABC subunit C

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1058 [new locus tag: NWMN_RS05980 ]
• symbol: uvrC
• product: excinuclease ABC subunit C
• replicon: chromosome
• strand: +
• coordinates: 1162417..1164198
• length: 1782
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332571 NCBI
• RefSeq: YP_001332092 NCBI
• BioCyc:
• MicrobesOnline: 3706609 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGGAAGACTATAAGCAACGAATTAAAAATAAATTAAATGTCGTACCTATGGAACCAGGA
  TGCTATTTAATGAAAGATCGTAATGATCAAGTGATATATGTTGGCAAAGCTAAAAAGCTA
  AGAAATCGATTGCGATCATATTTTACGGGTGCTCATGATGCTAAAACAACGAGACTGGTT
  GGTGAAATACGTCGCTTTGAGTTTATTGTCACGTCAAGTGAAACAGAATCACTTTTACTG
  GAATTGAATCTGATTAAACAATATCAACCAAGATATAATATATTATTAAAGGATGATAAA
  AGTTATCCATTTATTAAAATTACGAAGGAGAAATATCCTAGACTACTAGTGACGAGAACT
  GTAAAACAAGGTACTGGCAAATATTTCGGACCGTATCCGAATGCATATTCTGCTCAAGAA
  ACTAAAAAGTTATTAGACAGAATATATCCATATCGCAAATGTGATAAGATGCCAGATAAA
  TTATGTCTTTATTACCATATTGGACAATGTTTAGGACCATGTGTATATGACGTTGATTTG
  AGTAAATACGCACAAATGACGAAGGAAATTACTGATTTTCTGAATGGGGAAGACAAAACA
  ATTTTAAAAAGTTTAGAAGAGCGAATGTTAACTGCAAGTGAATCACTTGATTTTGAACGG
  GCTAAAGAATATAGAGATTTAATTCAACATATTCAAAATCTGACAAACAAACAAAAAATT
  ATGTCATCAGATAAAACGATTCGTGATGTCTTTGGTTATAGTGTTGATAAAGGATGGATG
  TGTATCCAAGTTTTCTTTATACGACAAGGTAATATGATAAAGCGAGATACAACGATGATT
  CCATTACAGCAAACAGAAGAAGAAGAATTTTATACATTTATTGGACAATTTTATAGCTTA
  AACCAACATATTTTACCTAAGGAAGTTCATGTACCACGTAATTTGGATAAAGAAATGATT
  CAATCTGTTGTGGACACTAAAATCGTTCAACCCGCGCGAGGTCCCAAAAAAGATATGGTT
  GACCTAGCTGCACATAACGCTAAAGTATCCTTAAATAATAAATTTGAATTAATATCACGT
  GATGAGTCCAGAACGATTAAAGCTATTGAAGAACTTGGAACACAAATGGGAATTCAAACA
  CCAATTAGAATTGAAGCATTCGATAATTCTAATATTCAAGGTGTGGATCCAGTGTCAGCA
  ATGGTTACATTTGTCGACGGTAAACCAGATAAGAAAAATTATAGAAAGTATAAAATCAAA
  ACGGTTAAAGGTCCAGATGATTACAAATCAATGAGAGAAGTAGTAAGACGACGATATTCT
  CGCGTTTTAAACGAAGGATTACCATTACCTGATTTAATAATAGTAGATGGTGGTAAAGGA
  CATATGAACGGGGTTATTGATGTGCTACAAAACGAATTAGGTCTTGATATCCCTGTTGCA
  GGTTTGCAGAAAAATGATAAACACCAAACATCTGAATTATTATATGGCGCTAGTGCAGAA
  ATTGTACCACTGAAGAAAAATAGCCAGGCATTTTATTTGTTGCACCGTATCCAAGATGAG
  GTTCACAGATTCGCAATCACATTTCATAGACAAACACGTCAAAAGACAGGCTTGAAATCA
  ATACTTGATGATATAGATGGTATCGGTAACAAACGTAAAACATTATTATTGCGTTCATTC
  GGTTCAATCAAGAAAATGAAGGAAGCTACACTTGAAGATTTTAAAAATATAGGTATTCCT
  GAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1058 [new locus tag: NWMN_RS05980 ]
• symbol: UvrC
• description: excinuclease ABC subunit C
• length: 593
• theoretical pI: 9.78595
• theoretical MW: 68670.9
• GRAVY: -0.577234

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit C (TIGR00194; EC 3.1.25.-; HMM-score: 660.4)
  and 1 more
  group I intron endonuclease (TIGR01453; HMM-score: 13.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Endonuclease (CL0189) UvrC_HhH_N; UvrC Helix-hairpin-helix N-terminal (PF08459; HMM-score: 179.3)
  and 4 more
  GIY-YIG (CL0418) GIY-YIG; GIY-YIG catalytic domain (PF01541; HMM-score: 44.9)
  no clan defined UVR; UvrB/uvrC motif (PF02151; HMM-score: 40)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 18.4)
  no clan defined DUF3936; Protein of unknown function (DUF3936) (PF13072; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00368
  • TAT(Tat/SPI): 0.000393
  • LIPO(Sec/SPII): 0.001302
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221270 NCBI
• RefSeq: YP_001332092 NCBI
• UniProt: A6QG48 UniProt

⊟Protein sequence

• MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRLRSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSYPFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDKLCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDFERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRDTTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARGPKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNIQGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLPDLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNSQAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKMKEATLEDFKNIGIPENVAKNLHEQLHK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications