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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001353
  • pan locus tag?: SAUPAN003764000
  • symbol: trpD
  • pan gene symbol?: trpD
  • synonym:
  • product: anthranilate phosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001353
  • symbol: trpD
  • product: anthranilate phosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1368219..1369217
  • length: 999
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACATTACTAACAAGAATAAAAACTGAAACTATATTACTTGAAAGCGACATTAAAGAG
    CTAATCGATATACTTATTTCTCCTAGTATTGGAACTGATATTAAATATGAATTACTTAGT
    TCCTATTCGGAGCGAGAAATCCAACAACAAGAATTAACATATATTGTACGTAGCTTAATT
    AATACAATGTATCCACATCAACCATGTTATGAAGGGGCTATGTGTGTGTGCGGCACAGGT
    GGTGACAAGTCAAATAGTTTCAACATTTCAACGACTGTAGCTTTTGTCGTAGCTAGTGCT
    GGCATAAAAGTTATAAAACATGGTAATAAAAGTATTACCTCAAATTCAGGTAGTACGGAT
    TTGTTAAATCAAATGAACATACAAACAACAACTGTTGATGATACACCTAACCAATTAAAT
    GAAAAAGACCTTGTATTCATTGGTGCAACTGAATCATATCCAATCATGAAGTATATGCAA
    CCAGTTAGAAAAATGATTGGAAAGCCTACAATATTAAACCTTGTGGGTCCATTAATTAAT
    CCATATCACTTAACGTATCAAATGGTAGGCGTCTTTGATCCTACAAAGTTAAAGTTAGTT
    GCTAAAACGATTAAAGATTTAGGCAGAAAACGTGCAATCGTTTTACATGGTGCAAATGGT
    ATGGATGAAGCAACACTATCTGGTGATAATTTGATATATGAATTGACTGAAGATGGAGAA
    ATCAAAAATTACACATTAAATGCGACTGATTATGGTTTGAAATATGCACCGAATAGTGAT
    TTTAAAGGTGGTTCACCTGAAGAAAACTTAGCAATCACACTGAATATTTTAAATGGTAAT
    GATCGCTCAAGTCGACGTGATGTGGTTGTCTTAAATGCAGGTTTAAGCCTATATGTTGCA
    GAGAAAGTGGATACCATCGCAGAAGGCATAGAACTTGCAACTACATTGATTGATAATGGT
    GAAGCATTGAAAAAATACCATCAAATGAGAGGTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001353
  • symbol: TrpD
  • description: anthranilate phosphoribosyltransferase
  • length: 332
  • theoretical pI: 5.08214
  • theoretical MW: 36579.6
  • GRAVY: -0.188855

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.18?  ExPASy
    Anthranilate phosphoribosyltransferase N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate phosphoribosyltransferase (TIGR01245; EC 2.4.2.18; HMM-score: 292.5)
    and 3 more
    putative thymidine phosphorylase (TIGR02645; EC 2.4.2.4; HMM-score: 20.7)
    AMP phosphorylase (TIGR03327; EC 2.4.-.-; HMM-score: 20.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other pyrimidine-nucleoside phosphorylase (TIGR02644; EC 2.4.2.2; HMM-score: 17.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Glycos_transf_3; Glycosyl transferase family, a/b domain (PF00591; HMM-score: 258)
    and 2 more
    P-loop_NTPase (CL0023) CSM2; Shu complex component Csm2, DNA-binding (PF16834; HMM-score: 13)
    NADP_Rossmann (CL0063) iSirtuin; Inactive Sirtuin (PF24403; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9952
    • Cytoplasmic Membrane Score: 0.0014
    • Cell wall & surface Score: 0.0008
    • Extracellular Score: 0.0026
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003672
    • TAT(Tat/SPI): 0.00015
    • LIPO(Sec/SPII): 0.000494
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSEREIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGIKVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQPVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGANGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKYAPNSDFKGGSPEENLAITLNILNGNDRSSRRDVVVLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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