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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1405 [new locus tag: SACOL_RS07150 ]
  • pan locus tag?: SAUPAN003764000
  • symbol: trpD
  • pan gene symbol?: trpD
  • synonym:
  • product: anthranilate phosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1405 [new locus tag: SACOL_RS07150 ]
  • symbol: trpD
  • product: anthranilate phosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1416185..1417183
  • length: 999
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACATTACTAACAAGAATAAAAACTGAAACTATATTACTTGAAAGCGACATTAAAGAG
    CTAATCGATATACTTATTTCTCCTAGTATTGGAACTGATATTAAATATGAATTACTTAGT
    TCCTATTCGGAGCGAGAAATCCAACAACAAGAATTAACATATATTGTACGTAGCTTAATT
    AATACAATGTATCCACATCAACCATGTTATGAAGGGGCTATGTGTGTGTGCGGCACAGGT
    GGTGACAAGTCAAATAGTTTCAACATTTCAACGACTGTTGCTTTTGTTGTAGCAAGTGCT
    GGCGTAAAAGTTATAAAACATGGTAATAAAAGTATTACCTCAAATTCAGGTAGTACGGAT
    TTGTTAAATCAAATGAACATACAAACAACAACTGTTGATGATACACCTAACCAATTAAAT
    GAAAAAGACCTTGTATTCATTGGTGCAACTGAATCATATCCAATCATGAAGTATATGCAA
    CCAGTTAGAAAAATGATTGGAAAGCCTACAATATTAAACCTTGTGGGTCCATTAATTAAT
    CCATATCACTTAACGTATCAAATGGTAGGCGTCTTTGATCCTACAAAGTTAAAGTTAGTT
    GCTAAAACGATTAAAGATTTAGGTAGAAAACGTGCAATCGTTTTACATGGTGCAAATGGT
    ATGGATGAAGCAACACTATCTGGTGATAATTTGATATATGAATTGACTGAAGATGGAGAA
    ATCAAAAATTACACATTAAATGCGACTGATTATGGTTTGAAACATGCGCCGAATAGTGAT
    TTTAAAGGCGGTTCACCTGAAGAAAATTTAGCAATCTCCCTTAATATCTTGAATGGTAAA
    GATCAGTCAAGTCGACGTGATGTTGTCTTACTAAATGCGGGTTTAAGCCTTTATGTTGCA
    GAGAAAGTGGATACCATCGCAGAAGGCATAGAACTTGCAACTACATTGATTGATAATGGT
    GAAGCATTGGAAAAATACCATCAAATGAGAGGTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1405 [new locus tag: SACOL_RS07150 ]
  • symbol: TrpD
  • description: anthranilate phosphoribosyltransferase
  • length: 332
  • theoretical pI: 4.96694
  • theoretical MW: 36526.5
  • GRAVY: -0.193976

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.18?  ExPASy
    Anthranilate phosphoribosyltransferase N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate phosphoribosyltransferase (TIGR01245; EC 2.4.2.18; HMM-score: 293.8)
    and 3 more
    putative thymidine phosphorylase (TIGR02645; EC 2.4.2.4; HMM-score: 20.8)
    AMP phosphorylase (TIGR03327; EC 2.4.-.-; HMM-score: 20.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other pyrimidine-nucleoside phosphorylase (TIGR02644; EC 2.4.2.2; HMM-score: 18.3)
  • TheSEED  :
    • Anthranilate phosphoribosyltransferase (EC 2.4.2.18)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Anthranilate phosphoribosyltransferase (EC 2.4.2.18)
  • PFAM:
    no clan defined Glycos_transf_3; Glycosyl transferase family, a/b domain (PF00591; HMM-score: 265.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003672
    • TAT(Tat/SPI): 0.00015
    • LIPO(Sec/SPII): 0.000494
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSEREIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGVKVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQPVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGANGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNILNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: T-box(Trp) (transcription antitermination) regulon, CodY (repression) regulon
    T-box(Trp)(RNA)important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

H De Lencastre, S W Wu, M G Pinho, A M Ludovice, S Filipe, S Gardete, R Sobral, S Gill, M Chung, A Tomasz
Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin.
Microb Drug Resist: 1999, 5(3);163-75
[PubMed:10566865] [WorldCat.org] [DOI] (P p)

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