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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001352
  • pan locus tag?: SAUPAN003763000
  • symbol: JSNZ_001352
  • pan gene symbol?: trpG
  • synonym:
  • product: aminodeoxychorismate/anthranilate synthase component II

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001352
  • symbol: JSNZ_001352
  • product: aminodeoxychorismate/anthranilate synthase component II
  • replicon: chromosome
  • strand: +
  • coordinates: 1367647..1368213
  • length: 567
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATCTTAGTTGTAGATAATTATGATTCCTTTACATATAACCTAGTGGATATTGTTGCT
    CAACATACTGACGTCATTGTTCAATACCCTGATGATGATAATGTGCTGAATCAATCGGTG
    GACGCTGTTATTATATCTCCTGGTCCAGGGCATCCATTAGACGATCAACAGTTGATGAAA
    ATCATATCAACCTATCAACACAAACCCATTTTAGGTATATGTTTAGGGGCTCAGGCACTG
    ACTTGTTACTACGGTGGAGAAGTCATTAAAGGCGACAAGGTTATGCACGGCAAAGTTGAT
    ACTATAAGTATCACTTCAAATACTCACAAAGTGTTATATCAAGATGTTCCAGAACAGTTT
    TCAATTATGAGATATCATTCATTAATAAGTAACCCTGACAATTTTCCAGAAGAATTGAAA
    ATTACTGGACGTACCAAAGATTGTATACAGTCATTTGAGCATAAAGAAAGACCGCATTAC
    GGTATTCAGTACCATCCTGAATCATTTGCTACAGACTATGGTGTCAAAATAATTACAAAT
    TTTATTAATCTAGTGAAGGAAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001352
  • symbol: JSNZ_001352
  • description: aminodeoxychorismate/anthranilate synthase component II
  • length: 188
  • theoretical pI: 5.18613
  • theoretical MW: 21241.9
  • GRAVY: -0.250532

⊟Function[edit | edit source]

  • TIGRFAM:
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 154.2)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 127.3)
    and 5 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 116.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 92.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 51.1)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 27.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 17.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 138.6)
    and 3 more
    Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 34.2)
    GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 14)
    KaiA_RbsU (CL0637) KaiA; KaiA C-terminal domain (PF07688; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9973
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0024
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007604
    • TAT(Tat/SPI): 0.000165
    • LIPO(Sec/SPII): 0.000607
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVIISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTISITSNTHKVLYQDVPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHYGIQYHPESFATDYGVKIITNFINLVKEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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