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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001356
  • pan locus tag?: SAUPAN003767000
  • symbol: trpB
  • pan gene symbol?: trpB
  • synonym:
  • product: tryptophan synthase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001356
  • symbol: trpB
  • product: tryptophan synthase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 1370626..1371840
  • length: 1215
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATAAACAAATACAAACAGAAGCAGATGAATTAGGTTTCTTTGGTGAATACGGAGGG
    CAATATGTTCCAGAAACATTAATGCCAGCAATTATTGAGTTGAAAAAAGCTTATAAAGAG
    GCAAAAGCAGACCCAGAGTTTCAAAGAGAACTGGAATACTATTTATCAGAGTATGTAGGA
    CGCGCGACGCCACTTACATATGCTGCATCATATACTGAAAGCCTAGGTGGCGCTAAAATA
    TATTTGAAACGAGAGGATCTAAATCATACAGGCGCCCATAAAATTAATAATGCGTTAGGT
    CAAGCGTTGCTTGCTAAAAGAATGGGCAAGAAGAAGCTTGTTGCTGAAACTGGTGCGGGT
    CAACATGGTGTAGCTAGTGCTACGGTTGCTGCATTATTTGATATGGAACTTGTTGTCTTT
    ATGGGAAGTGAAGATATTAAAAGACAACAACTTAATGTATTTAGAATGGAATTACTTGGT
    GCAAAGGTTGTGGCAGTTGAAGATGGTCAAGGGACTTTATCGGATGCAGTTAATAAAGCA
    TTGCAATATTGGGTAAGTCATGTAGATGATACACATTATTTATTAGGTTCTGCATTAGGT
    CCAGACCCGTTCCCAACGATTGTTAGAGATTTTCAGAGTGTGATTGGTAAAGAAATAAAA
    TCACAGATATTGAAGAAAGAAGGTCGACTTCCGGATGCAATTGTAGCATGTATCGGTGGT
    GGCTCAAATGCAATCGGTACATTTTATCCATTTATTAAAGATGATGTTGCATTATACGGT
    GTTGAAGCCGCAGGTCAAGGCGAGGATACTGATAAACATGCACTTGCAATTGGCAAAGGA
    TCACCTGGCGTATTACATGGTACTAAAATGTATTTAATTCAAGATGAAGATGGGCAAGTG
    CAACTAGCACATTCTATTTCAGCAGGACTTGATTATCCTGGTATTGGACCAGAACATTCT
    TATTACCACGACATTGGTAGAGTAACTTTTGAAAATGCAAGTGATACACAAGCAATGAAT
    GCTTTAATCAACTTTACAAAACATGAAGGTATTATACCTGCAATTGAAAGTGCACATGCA
    CTGAGTTATGTTGAAAGACTAGCGCCTACGATGTCGAAAGAAGATATTATTGTAGTAACT
    ATTTCTGGACGTGGCGATAAAGATATGGAAACAATTAGACAATATATGGCAGAGCGAGGT
    CTTGCAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1215

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001356
  • symbol: TrpB
  • description: tryptophan synthase subunit beta
  • length: 404
  • theoretical pI: 5.07847
  • theoretical MW: 43950.5
  • GRAVY: -0.229703

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.20?  ExPASy
    Tryptophan synthase L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 650.6)
    and 11 more
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 181.8)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 44.5)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 32.1)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 30.7)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 30.1)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 29.5)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 28.5)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 26.7)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 24.4)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 16.1)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 14.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 127.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.927
    • Cytoplasmic Membrane Score: 0.0399
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0322
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00892
    • TAT(Tat/SPI): 0.000876
    • LIPO(Sec/SPII): 0.001005
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKADPEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLGAKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKEIKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALAIGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENASDTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETIRQYMAERGLAND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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