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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001038
  • pan locus tag?: SAUPAN003286000
  • symbol: purH
  • pan gene symbol?: purH
  • synonym:
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001038
  • symbol: purH
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1041584..1043062
  • length: 1479
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCG
    TTAACGCAATTAAATTATGAATTATATTCAACAGGTGGTACTAAACGTATATTAGATGAA
    GCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATTTTCCAGAAATAATGGATGGC
    CGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
    CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAAT
    TTATATCCATTCCAACAAACTGTTGCAAACCCTGATGTAACGATGGACGAAGCAATTGAA
    AATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAGCTAAAAACTATAAACATGTA
    ACAACAATTGTACATCCTGCAGATTATCAAGAAGTATTGACGCGATTAAGAAACGATTCG
    TTAGATGAGTCATATAGACAGTCATTAATGATCAAAGTTTTTGAGCATACTGCAGAATAT
    GATGAAGCGATTGTACGTTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAAT
    CCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTAAGCACACGATTGCAGGCGCT
    AAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
    GCTTTAGTTAAAAAGTTTGATACACCTGCTGCAGTTGCGGTTAAACACATGAATCCATGT
    GGTGTTGGTATCGGTGACACGATTGAACAAGCATTTCAACATGCGTATGAAGCGGATAGT
    CAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTGTAACACCTGAGTTAGCAGAG
    CAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
    GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGT
    AACGAAGAAGAGTTTGTTTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTAT
    GTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAGCACCTACTGATGAACAATGG
    GAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
    AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCT
    AAAATTGCGTTAGAGAGAGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGA
    TTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAACATGGTATAAAGGCAATTATC
    CAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
    GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001038
  • symbol: PurH
  • description: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • length: 492
  • theoretical pI: 5.92514
  • theoretical MW: 54317.5
  • GRAVY: -0.219106

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.3?  ExPASy
    Phosphoribosylaminoimidazolecarboxamide formyltransferase 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
    EC 3.5.4.10?  ExPASy
    IMP cyclohydrolase IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 644.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    CDA (CL0109) AICARFT_IMPCHas; AICARFT/IMPCHase bienzyme (PF01808; HMM-score: 380.3)
    and 3 more
    no clan defined MGS; MGS-like domain (PF02142; HMM-score: 97.7)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 14.8)
    Hybrid (CL0105) NSP2_assoc; Non-essential region of nsp2 of arterivirus polyprotein (PF14758; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.994
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.0026
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018646
    • TAT(Tat/SPI): 0.00094
    • LIPO(Sec/SPII): 0.002184
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDSLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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