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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001036
  • pan locus tag?: SAUPAN003284000
  • symbol: purM
  • pan gene symbol?: purM
  • synonym:
  • product: phosphoribosylformylglycinamidine cyclo-ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001036
  • symbol: purM
  • product: phosphoribosylformylglycinamidine cyclo-ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1039972..1041000
  • length: 1029
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTAAAGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAA
    AGAATGTCTAGTCATGTTAAACGTACGATGCGTAAAGAAGTTATCGGTGGTTTAGGTGGA
    TTCGGTGCTACATTTGATTTATCACAATTAAATATGACAGCACCAATTTTAGTTTCTGGA
    ACAGACGGAGTAGGTACGAAATTAAAACTAGCTATCGACTATGGGAAACATGATTCGATA
    GGTATCGATGCAGTCGCAATGTGTGTTAATGATATTTTAACGACAGGTGCAGAACCATTG
    TATTTTTTAGATTACATCGCTACAAACAAAGTTGTTCCTGAAGTTATTGAACAAATTGTT
    AAAGGTATTAGTGATGCATGCGTTGAAACGAATACTGCACTTATCGGTGGAGAGACTGCT
    GAAATGGGTGAAATGTATCACGAAGGTGAATATGATGTAGCCGGATTTGCAGTTGGAGCA
    GTTGAAAAGGATGACTATGTAGATGGTTCAGAAGTGAAAGAGGGACAAGTTGTTATAGGG
    CTTGCGTCAAGTGGCATTCATTCAAATGGATATAGTTTAGTGCGCAAATTAATTAATGAA
    TCAGGCATTGATTTGGCATCAAACTTCGATAATCGTCCATTTATAGATGTCTTTTTAGAA
    CCAACTAAATTATATGTCAAACCTGTACTTGCTTTAAAAAAAGAAGTTTCTATTAAGGCA
    ATGAATCATATTACTGGTGGAGGCTTTTATGAAAATATTCCACGTGCATTGTCAGCCGGA
    TATGCTGCTAGAATTGATACTACATCATTTCCAACGCCAAAAATATTTGATTGGTTACAA
    CAACAAGGCAATATAGACACAAATGAAATGTATAACATTTTTAACATGGGTATTGGCTAT
    ACGGTTATCGTTGATGAAAAAGATGCATCACGCGCTTTGAAGATTTTAGCAGAACAAAAT
    GTGGAAGCTTATCAAATTGGTCATATTGTGAAAAATGAGTCAACTGCAATTGAATTGTTG
    GGGGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001036
  • symbol: PurM
  • description: phosphoribosylformylglycinamidine cyclo-ligase
  • length: 342
  • theoretical pI: 4.52335
  • theoretical MW: 36992.8
  • GRAVY: -0.021345

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.3.1?  ExPASy
    Phosphoribosylformylglycinamidine cyclo-ligase ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 426.9)
    and 8 more
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 45.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 43.4)
    Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 32.4)
    selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 25.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 22.3)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 17.5)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 17.1)
    bacteriocin-type signal sequence (TIGR01847; HMM-score: 11)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 135.7)
    and 1 more
    PurM_N-like (CL0804) AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 89)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9864
    • Cytoplasmic Membrane Score: 0.0029
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0104
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.023694
    • TAT(Tat/SPI): 0.020155
    • LIPO(Sec/SPII): 0.00238
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGATFDLSQLNMTAPILVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYFLDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGAVEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVFLEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALSAGYAARIDTTSFPTPKIFDWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNESTAIELLGV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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