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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001030
  • pan locus tag?: SAUPAN003278000
  • symbol: purK
  • pan gene symbol?: purK
  • synonym:
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001030
  • symbol: purK
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1033566..1034690
  • length: 1125
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTTCAATAAATTAAAGTTTGGTGCGACTATTGGCATTATTGGTGGTGGTCAGCTT
    GGAAAGATGATGGCACAATCAGCTCAAAAAATGGGTTATAAAGTGGTTGTATTGGATCCT
    TCTGAAGATTGTCCATGTAGATACGTTGCACACGAATTTATACAAGCCAAGTATGACGAT
    GAAAAGGCACTCAATCAATTAGGACAAAAATGTGATGTGATTACTTATGAATTTGAAAAC
    ATTTCAGCCCAACAATTAAAACTATTATGTGAAAAGTACAATATTCCGCAAGGTTACCAA
    GCTATACAGTTATTACAAGATCGCTTAACTGAAAAAGAAACATTAAAAAGTGCTGGTACC
    AAAGTTGTCCCGTTCATTTCAGTAAAAGAATCTACAGATATTGACAAAGCAATTGAAACA
    TTAGGATATCCTTTTATTGTAAAAACTAGATTTGGTGGCTACGATGGCAAAGGTCAAGTT
    TTAATTAACAACGAAAAAGACTTACAAGAAGGTTTTAAATTAATTGAAACTAGTGAATGC
    GTAGCTGAAAAATATTTGAATATCAAGAAAGAAGTATCTCTTACTGTTACAAGAGGAAAC
    AACAATCAAATCACTTTTTTCCCATTACAAGAAAATGAGCATAGAAATCAAATACTTTTC
    AAAACAATTGTTCCAGCGAGAATAGATAAAACAGCTGAGGCGAAAGAGCAAGTTAATAAA
    ATTATCCAATCGATTCATTTCATTGGAACATTTACAGTTGAATTTTTTATAGATAGTAAC
    AACCAATTGTATGTGAACGAGATAGCACCAAGGCCTCACAATTCCGGACATTATTCAATT
    GAAGCATGTGATTATTCACAATTTGATACTCATATTTTAGCAGTTACCGGACAATCATTA
    CCAAATTCAATTGAATTATTAAAGCCAGCAGTCATGATGAACTTACTAGGTAAAGATTTA
    GATTTATTGGAAAATGAATTTAATGAACATCCAGAGTGGCACTTACATATTTATGGTAAG
    TCTGAGCGTAAAGATAGCAGAAAAATGGGGCATATGACTGTACTAACGAATGATGTAAAC
    CAAACTGAACAAGATATGTACGCTAAATTTGAGGGGAGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

Protein Data Bank: 3ORQ
Protein Data Bank: 3ORR

⊟General[edit | edit source]

  • locus tag: JSNZ_001030
  • symbol: PurK
  • description: 5-(carboxyamino)imidazole ribonucleotide synthase
  • length: 374
  • theoretical pI: 5.8331
  • theoretical MW: 42493.1
  • GRAVY: -0.437968

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide synthase ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 385.4)
    and 11 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 112.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 38.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 37.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 37.1)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 35.9)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 29.9)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 23.4)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 20.4)
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 18.8)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 18.4)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 11.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 169.6)
    and 26 more
    NADP_Rossmann (CL0063) RS_preATP-grasp-like; Ribonucleotide synthetase preATP-grasp domain (PF22660; HMM-score: 109.4)
    Hybrid (CL0105) PurK_C; Phosphoribosylaminoimidazole carboxylase C-terminal domain (PF17769; HMM-score: 62.7)
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 48.4)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 26.9)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 26.5)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 26.5)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 23.1)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 22.7)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.5)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 20.3)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 19.7)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 18.2)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 17.3)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 16.1)
    PglD_N; PglD N-terminal domain (PF17836; HMM-score: 15.9)
    PylC-like_N; PylC-like, N-terminal domain (PF21360; HMM-score: 14.8)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 14.4)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.4)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 13.8)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 13.3)
    HI0933_like; HI0933-like protein Rossmann domain (PF03486; HMM-score: 13.3)
    LpxI_N; LpxI N-terminal domain (PF17930; HMM-score: 13.2)
    ATP-grasp (CL0179) DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 13.1)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 12.9)
    NADP_Rossmann (CL0063) NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 12.6)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9718
    • Cytoplasmic Membrane Score: 0.0161
    • Cell wall & surface Score: 0.0016
    • Extracellular Score: 0.0105
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.243947
    • TAT(Tat/SPI): 0.024705
    • LIPO(Sec/SPII): 0.016745
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQVLINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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