From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001035
  • pan locus tag?: SAUPAN003283000
  • symbol: purF
  • pan gene symbol?: purF
  • synonym:
  • product: amidophosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001035
  • symbol: purF
  • product: amidophosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1038495..1039979
  • length: 1485
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCT
    GAAGCAGCGCAACTAACATATATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGT
    GCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCGAGCGAGGATTAGGCTTACTA
    ACTGAAGCGATTAAAGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
    CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTAT
    CACTTTTATGATATGAGTGTAGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCA
    TTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATTCGTCTTCTGATACTGAAGTC
    ATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTAAAAGAAAGT
    TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATATTAACTAAAGATGCGTTATATGGC
    GCAGTAGATCCAAATGCTATCAGACCACTTGTTGTAGGTAAAATGAAAGATGGTACATAC
    ATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTGCAGAATTTGTTCAAGATATT
    CATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
    CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACA
    ATAGCTGGTAAAAATGTCCATGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAA
    AGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATTCATCGCTATCAGCTGCGAGT
    GGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
    GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTA
    TCTGCGGTAAAAGATATAGTAGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTT
    CGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAGATTCTGGTGCAAATAAAGTA
    CATGTGCGTATAGCATCACCGGAATTTATGTTCCCAAGTTTTTATGGAATCGATGTTTCA
    ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGC
    GCTGATTCATTAGCATATCTATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTAT
    GACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTGGAGATTATCCTGCAGGGTTA
    TATGATTATGAAGCAAATTATAAAGCGCATTTAAGTCATCGACAAAAGCAATATATTTCT
    AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001035
  • symbol: PurF
  • description: amidophosphoribosyltransferase
  • length: 494
  • theoretical pI: 6.56199
  • theoretical MW: 54396.3
  • GRAVY: -0.229555

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.14?  ExPASy
    Amidophosphoribosyltransferase 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 485.1)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 75.8)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 47.5)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 31.3)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 19.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 16.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NTN (CL0052) GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 70.2)
    GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 68.5)
    and 6 more
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 39.5)
    NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 22.1)
    PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 17.7)
    PRTase-CE; PRTase-CE (PF24390; HMM-score: 13.8)
    PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 13)
    no clan defined ArgZ-like_C_1st; Arginine dihydrolase ArgZ-like, C-terminal, first region (PF21571; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8138
    • Cytoplasmic Membrane Score: 0.022
    • Cell wall & surface Score: 0.0024
    • Extracellular Score: 0.1618
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003038
    • TAT(Tat/SPI): 0.000224
    • LIPO(Sec/SPII): 0.000463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_001035&oldid=106676"