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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001035
  • pan locus tag?: SAUPAN003283000
  • symbol: purF
  • pan gene symbol?: purF
  • synonym:
  • product: amidophosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001035
  • symbol: purF
  • product: amidophosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1038495..1039979
  • length: 1485
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCT
    GAAGCAGCGCAACTAACATATATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGT
    GCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCGAGCGAGGATTAGGCTTACTA
    ACTGAAGCGATTAAAGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
    CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTAT
    CACTTTTATGATATGAGTGTAGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCA
    TTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATTCGTCTTCTGATACTGAAGTC
    ATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTAAAAGAAAGT
    TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATATTAACTAAAGATGCGTTATATGGC
    GCAGTAGATCCAAATGCTATCAGACCACTTGTTGTAGGTAAAATGAAAGATGGTACATAC
    ATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTGCAGAATTTGTTCAAGATATT
    CATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
    CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACA
    ATAGCTGGTAAAAATGTCCATGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAA
    AGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATTCATCGCTATCAGCTGCGAGT
    GGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
    GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTA
    TCTGCGGTAAAAGATATAGTAGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTT
    CGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAGATTCTGGTGCAAATAAAGTA
    CATGTGCGTATAGCATCACCGGAATTTATGTTCCCAAGTTTTTATGGAATCGATGTTTCA
    ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGC
    GCTGATTCATTAGCATATCTATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTAT
    GACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTGGAGATTATCCTGCAGGGTTA
    TATGATTATGAAGCAAATTATAAAGCGCATTTAAGTCATCGACAAAAGCAATATATTTCT
    AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001035
  • symbol: PurF
  • description: amidophosphoribosyltransferase
  • length: 494
  • theoretical pI: 6.56199
  • theoretical MW: 54396.3
  • GRAVY: -0.229555

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.14?  ExPASy
    Amidophosphoribosyltransferase 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 485.1)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 75.8)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 47.5)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 31.3)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 19.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 16.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 13.7)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NTN (CL0052) GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 70.2)
    GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 68.5)
    and 6 more
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 39.5)
    NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 22.1)
    PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 17.7)
    PRTase-CE; PRTase-CE (PF24390; HMM-score: 13.8)
    PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 13)
    no clan defined ArgZ-like_C_1st; Arginine dihydrolase ArgZ-like, C-terminal, first region (PF21571; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8138
    • Cytoplasmic Membrane Score: 0.022
    • Cell wall & surface Score: 0.0024
    • Extracellular Score: 0.1618
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003038
    • TAT(Tat/SPI): 0.000224
    • LIPO(Sec/SPII): 0.000463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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