From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01014
  • pan locus tag?: SAUPAN003283000
  • symbol: SAOUHSC_01014
  • pan gene symbol?: purF
  • synonym:
  • product: amidophosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01014
  • symbol: SAOUHSC_01014
  • product: amidophosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 984720..986204
  • length: 1485
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCT
    GAAGCAGCGCAACTAACATATATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGT
    GCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCGAGCGAGGATTAGGCTTACTA
    ACTGAAGCGATTAAAGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
    CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTAT
    CACTTTTATGATATGAGTGTAGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCA
    TTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATTCGTCTTCTGATACTGAAGTC
    ATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTGAAAGAAAGT
    TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATTTTAACTAAAGATGCGTTATATGGC
    GCAGTAGATCCAAATGCTATCAGACCACTTGTTGTAGGTAAAATGAAAGATGGGACATAC
    ATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTGCAGAATTTGTTCAAGATATT
    CATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
    CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACA
    ATAGCTGGTAAAAATGTCCATGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAA
    AGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATTCATCGCTATCAGCTGCGAGT
    GGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
    GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTA
    TCTGCGGTAAAAGATATAGTAGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTT
    CGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAGATTCTGGTGCAAATAAAGTG
    CATGTGCGTATAGCATCACCGGAATTTATGTTTCCAAGTTTTTACGGAATCGATGTTTCA
    ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGC
    GCTGATTCATTAGCATATCTATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTAT
    GACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTGGAGATTATCCTGCAGGGTTA
    TATGATTATGAAGCAAATTATAAAGCGCATTTAAGTCATCGACAAAAGCAATATATTTCT
    AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01014
  • symbol: SAOUHSC_01014
  • description: amidophosphoribosyltransferase
  • length: 494
  • theoretical pI: 6.56199
  • theoretical MW: 54396.3
  • GRAVY: -0.229555

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.14?  ExPASy
    Amidophosphoribosyltransferase 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 485.1)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 75.8)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 47.5)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 31.3)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 19.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 16.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 13.7)
  • TheSEED  :
    • Amidophosphoribosyltransferase (EC 2.4.2.14)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Amidophosphoribosyltransferase (EC 2.4.2.14)
    and 1 more
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Amidophosphoribosyltransferase (EC 2.4.2.14)
  • PFAM:
    NTN (CL0052) GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 71.9)
    GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 66.3)
    and 4 more
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 40.6)
    NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 22.3)
    PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 16.4)
    PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003038
    • TAT(Tat/SPI): 0.000224
    • LIPO(Sec/SPII): 0.000463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_00195acetyl-CoA acetyltransferase  [3] (data from MRSA252)
    SAOUHSC_00196hypothetical protein  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_01014&oldid=67208"