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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01017
  • pan locus tag?: SAUPAN003286000
  • symbol: purH
  • pan gene symbol?: purH
  • synonym:
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01017
  • symbol: purH
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 987809..989287
  • length: 1479
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCG
    TTAACGCAATTAAATTATGAATTATATTCAACAGGTGGTACTAAACGTATATTAGATGAA
    GCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATTTTCCAGAAATAATGGATGGC
    CGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
    CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAAT
    TTATATCCATTCCAACAAACTGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAA
    AATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAGCTAAAAACTATAAACATGTA
    ACAACAATTGTACATCCGGCAGATTATCAAGAAGTATTGACGCGATTAAGAAACGATTCG
    TTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAATAT
    GATGAAGCGATTGTACGTTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAAT
    CCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTAAGCACACGATTGCAGGCGCT
    AAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
    GCTTTAGTTAAAAAGTTTGATACACCTGCTACAGTTGCGGTTAAACACATGAATCCATGT
    GGTGTTGGTATCGGTGACACGATAGAACAAGCATTTCAACATGCGTATGAAGCGGATAGT
    CAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTGTAACACCTGAGTTAGCAGAG
    CAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
    GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGT
    AACGAAGAAGAGTTTGTTTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTAT
    GTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAGCACCTACAGATGAACAATGG
    GAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
    AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCT
    AAAATTGCGTTAGAGAGAGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGA
    TTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAACATGGTATAAAGGCAATTATC
    CAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
    GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01017
  • symbol: PurH
  • description: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • length: 492
  • theoretical pI: 5.92514
  • theoretical MW: 54347.5
  • GRAVY: -0.224187

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.3?  ExPASy
    Phosphoribosylaminoimidazolecarboxamide formyltransferase 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
    EC 3.5.4.10?  ExPASy
    IMP cyclohydrolase IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 643.4)
  • TheSEED  :
    • IMP cyclohydrolase (EC 3.5.4.10)
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  IMP cyclohydrolase (EC 3.5.4.10)
    and 1 more
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)
  • PFAM:
    CDA (CL0109) AICARFT_IMPCHas; AICARFT/IMPCHase bienzyme (PF01808; HMM-score: 361)
    and 3 more
    no clan defined MGS; MGS-like domain (PF02142; HMM-score: 106.4)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 17.7)
    EFTPs (CL0575) NSP2_assoc; Non-essential region of nsp2 of arterivirus polyprotein (PF14758; HMM-score: 15)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018646
    • TAT(Tat/SPI): 0.00094
    • LIPO(Sec/SPII): 0.002184
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDSLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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