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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0925 [new locus tag: SA_RS05240 ]
  • pan locus tag?: SAUPAN003286000
  • symbol: purH
  • pan gene symbol?: purH
  • synonym:
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0925 [new locus tag: SA_RS05240 ]
  • symbol: purH
  • product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1050088..1051569
  • length: 1482
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAA
    GCGTTAACGCAATTAAATTATGAATTATATTCAACTGGTGGTACTAAACGTATATTAGAT
    GAAGCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATTTTCCAGAAATAATGGAT
    GGCCGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAA
    CCGCAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTT
    AATTTATATCCATTCCAACAAACTGTTGCAAACCCTGATGTGACGATGGACGAAGCAATT
    GAAAATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAGCTAAAAACTATAAACAT
    GTAACAACAATTGTACATCCGGCAGATTATCATGAAGTATTGACGCGATTAAGAAACGAT
    TCGTTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAA
    TATGATGAAGCGATTGTACGTTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAA
    AATCCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTAAGCACACGATTGCAGGT
    GCTAAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACA
    CTAGCTTTAGTTAAAAAGTTTGATACACCTGCTGCAGTTGCGGTTAAACACATGAATCCA
    TGTGGTGTTGGTATCGGTGACACGATTGAACAAGCATTTCAACATGCGTATGAAGCGGAT
    AGTCAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTGTAACACCTGAGTTAGCA
    GAGCAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCA
    TTAGATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGAC
    AGTAACGAAGAAGAGTTTGTTTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAAT
    TATGTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAGCACCTACTGATGAACAA
    TGGGAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATT
    TTAAGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCT
    GCTAAAATTGCGTTAGAGAGAGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGAT
    GGATTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAACATGGTATAAAGGCAATT
    ATCCAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGT
    ATTGCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0925 [new locus tag: SA_RS05240 ]
  • symbol: PurH
  • description: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • length: 493
  • theoretical pI: 6.00984
  • theoretical MW: 54457.7
  • GRAVY: -0.214199

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.3?  ExPASy
    Phosphoribosylaminoimidazolecarboxamide formyltransferase 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
    EC 3.5.4.10?  ExPASy
    IMP cyclohydrolase IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 644.4)
  • TheSEED  :
    • IMP cyclohydrolase (EC 3.5.4.10)
    • Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  IMP cyclohydrolase (EC 3.5.4.10)
    and 1 more
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)
  • PFAM:
    CDA (CL0109) AICARFT_IMPCHas; AICARFT/IMPCHase bienzyme (PF01808; HMM-score: 361.4)
    and 3 more
    no clan defined MGS; MGS-like domain (PF02142; HMM-score: 106.3)
    PBP (CL0177) Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 16.9)
    EFTPs (CL0575) NSP2_assoc; Non-essential region of nsp2 of arterivirus polyprotein (PF14758; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018643
    • TAT(Tat/SPI): 0.001252
    • LIPO(Sec/SPII): 0.00219
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDSLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA0627hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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