PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0918 [new locus tag: SA_RS05205 ]
• pan locus tag^?: SAUPAN003279000
• symbol: purC
• pan gene symbol^?: purC
• synonym:
• product: phosphoribosylaminoimidazolesuccinocarboxamide synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0918 [new locus tag: SA_RS05205 ]
• symbol: purC
• product: phosphoribosylaminoimidazolesuccinocarboxamide synthase
• replicon: chromosome
• strand: +
• coordinates: 1043201..1043905
• length: 705
• essential: yes DEG other strains

⊟Accession numbers

• Gene ID: 1123741 NCBI
• RefSeq: NP_374185 NCBI
• BioCyc: see SA_RS05205
• MicrobesOnline: 103211 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACATTATTATATGAAGGAAAAGCGAAGCGCATTTTCTCAACAAATCAAGAAAATGAA
  TTAAGAGTTGAATATAAAGATGAAGTTACTGCTGGAAACGGGGCTAAGAAAGACACAATG
  GCAGGTAAGGGGCGATTAAATAATCAAATTACTTCTATTATATTTAAATATTTACAAGAA
  AATGGAATAGAAAGTCACTTTATTAAACAATTATCTGAAACAGAACAATTAGTTAAGCCT
  GTGAAAATAATTCCATTAGAAGTAGTTGTTCGTAATATTGCTAGTGGATCTATTACAAAG
  CGTTTAGGTTTTGAAAATGGTGAAGTTTTTAGAGAACCACTTGTAGAATTTTTCTATAAA
  AATGATGCGTTAAATGATCCGTTGATAACGGATGACCATGTTAAATTGCTCAATATAGCA
  TCAGATGAAGATATTGAAATACTAAAATCCAAAGCATTAAAGATTAATAATGTGTTGAAA
  CAATTAATGGATGCTATGAATTTAAAATTAGTAGATTTTAAAATCGAATTTGGAAAGACT
  GAGACTGGTCAAATTTTGTTAGCGGATGAAATATCTCCAGATACATGTCGAATTTGGGAT
  AAAGCTACCAATGCAAACTTTGATAAAGATGTATATAGAAATAACACTGGATCACTGATT
  GAAACATATCAAATATTTTTAAACAAATTGGAGGATTTAAAATAA
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⊟Protein

⊟General

• locus tag: SA0918 [new locus tag: SA_RS05205 ]
• symbol: PurC
• description: phosphoribosylaminoimidazolesuccinocarboxamide synthase
• length: 234
• theoretical pI: 5.07088
• theoretical MW: 26692.4
• GRAVY: -0.379915

⊟Function

• reaction:
  EC 6.3.2.6?  ExPASy
  Phosphoribosylaminoimidazolesuccinocarboxamide synthase ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazolesuccinocarboxamide synthase (TIGR00081; EC 6.3.2.6; HMM-score: 236.4)
  and 2 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole-succinocarboxamide synthase (TIGR02735; EC 6.3.2.6; HMM-score: 26.5)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 16.1)
• TheSEED  :
  • Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)
  Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)
• PFAM:
  no clan defined SAICAR_synt; SAICAR synthetase (PF01259; HMM-score: 212.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007291
  • TAT(Tat/SPI): 0.000705
  • LIPO(Sec/SPII): 0.001456
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926652 NCBI
• RefSeq: NP_374185 NCBI
• UniProt: P99064 UniProt

⊟Protein sequence

• MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKGRLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRLGFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLKQLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGSLIETYQIFLNKLEDLK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0916 > purK > purC > SA0919 > purQ > purL > purF > purM > purN > purH > purD

⊟Regulation

• regulator: PurR (repression) regulon

  PurR

  (TF)

  important in Purine metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)