PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0920 [new locus tag: SA_RS05215 ]
• pan locus tag^?: SAUPAN003281000
• symbol: purQ
• pan gene symbol^?: purQ
• synonym:
• product: phosphoribosylformylglycinamidine synthase I

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0920 [new locus tag: SA_RS05215 ]
• symbol: purQ
• product: phosphoribosylformylglycinamidine synthase I
• replicon: chromosome
• strand: +
• coordinates: 1044170..1044841
• length: 672
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123743 NCBI
• RefSeq: NP_374187 NCBI
• BioCyc: see SA_RS05215
• MicrobesOnline: 103213 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAATTTGCGGTTCTTGTTTTTCCAGGTTCGAATTGTGATAGAGACATGTTTAATGCT
  GCTATTAAAAGTGGTGTTGAAGCGGAATATGTAGATTATAGAGAAACATCACTAAGTGGA
  TTTGATGGCGTACTTATTCCTGGTGGATTTTCATTCGGGGATTACTTAAGATCTGGGGCA
  ATGGCTAGTGTAGCGCCGATTATTTCGGAAGTTAAACGTCTTGCAACTGAAGGTAAGCCA
  GTATTAGGTGTTTGTAATGGGTTTCAAATTTTAACTGAAATAGGCTTATTACCTGGTGCA
  TTATTGCATAACGATTCACATTTATTTATTAGTAGAAATGAAGAGTTAGAAATAGTGAAT
  AATCAAACGGCATTTACAAATCTTTATGAGCAAGGTGAAAAAGTTATATATCCTGTAGCT
  CACGGTGAAGGTCATTATTATTGTACTGATGAAATATATCAACAATTAAAAGCTAACAAT
  CAAATTATTCTGAAATATGTGAATAATCCGAACGGTTCATATGATGATATTGCAGGAATT
  GTTAACGAAAAAGGCAATGTATGTGGCATGATGCCACATCCTGAAAGAGCTTTAGAAACG
  TTGTTAGGTACTGATAGTGGTGTGAAATTATTTGAAGCGATGGTAAAAAGTTGGAGGGAA
  CAACATGTCTAA
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  672
  

⊟Protein

⊟General

• locus tag: SA0920 [new locus tag: SA_RS05215 ]
• symbol: PurQ
• description: phosphoribosylformylglycinamidine synthase I
• length: 223
• theoretical pI: 4.69597
• theoretical MW: 24556.7
• GRAVY: -0.120179

⊟Function

• reaction:
  EC 3.5.1.2?  ExPASy
  Glutaminase L-glutamine + H₂O = L-glutamate + NH₃
  EC 6.3.5.3?  ExPASy
  Phosphoribosylformylglycinamidine synthase ATP + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 325.5)
  and 9 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 82.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 76.7)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 26)
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 25.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 22)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 17.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 17.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 16.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 13.5)
• TheSEED  :
  • Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
  Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
• PFAM:
  Glutaminase_I (CL0014) GATase_5; CobB/CobQ-like glutamine amidotransferase domain (PF13507; HMM-score: 154.9)
  and 5 more
  GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 33.1)
  DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 30.3)
  GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 29.7)
  Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 15.9)
  SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.045141
  • TAT(Tat/SPI): 0.000764
  • LIPO(Sec/SPII): 0.006095
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926654 NCBI
• RefSeq: NP_374187 NCBI
• UniProt: P99166 UniProt

⊟Protein sequence

• MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGVLIPGGFSFGDYLRSGAMASVAPIISEVKRLATEGKPVLGVCNGFQILTEIGLLPGALLHNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANNQIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSWREQHV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0916 > purK > purC > SA0919 > purQ > purL > purF > purM > purN > purH > purD

⊟Regulation

• regulator: PurR (repression) regulon

  PurR

  (TF)

  important in Purine metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)