From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1079 [new locus tag: SACOL_RS05505 ]
  • pan locus tag?: SAUPAN003283000
  • symbol: purF
  • pan gene symbol?: purF
  • synonym:
  • product: amidophosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1079 [new locus tag: SACOL_RS05505 ]
  • symbol: purF
  • product: amidophosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1088320..1089804
  • length: 1485
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCT
    GAAGCAGCGCAACTAACATATATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGT
    GCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCGAGCGAGGATTAGGCTTACTA
    ACTGAAGCGATTAAAGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
    CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTAT
    CACTTTTATGATATGAGTGTAGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCA
    TTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATTCGTCTTCTGATACTGAAGTC
    ATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTGAAAGAAAGT
    TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATTTTAACTAAAGATGCGTTATATGGC
    GCAGTAGATCCAAATGCTATCAGACCACTTGTTGTAGGTAAAATGAAAGATGGGACATAC
    ATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTGCAGAATTTGTTCAAGATATT
    CATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
    CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACA
    ATAGCTGGTAAAAATGTCCATGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAA
    AGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATTCATCGCTATCAGCTGCGAGT
    GGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
    GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTA
    TCTGCGGTAAAAGATATAGTAGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTT
    CGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAGATTCTGGTGCAAATAAAGTG
    CATGTGCGTATAGCATCACCGGAATTTATGTTTCCAAGTTTTTACGGAATCGATGTTTCA
    ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGC
    GCTGATTCATTAGCATATCTATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTAT
    GACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTGGAGATTATCCTGCAGGGTTA
    TATGATTATGAAGCAAATTATAAAGCGCATTTAAGTCATCGACAAAAGCAATATATTTCT
    AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1485

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1079 [new locus tag: SACOL_RS05505 ]
  • symbol: PurF
  • description: amidophosphoribosyltransferase
  • length: 494
  • theoretical pI: 6.56199
  • theoretical MW: 54396.3
  • GRAVY: -0.229555

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.14?  ExPASy
    Amidophosphoribosyltransferase 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 485.1)
    and 10 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 116.1)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 75.8)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 47.5)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 31.3)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 19.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 16.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 13.7)
  • TheSEED  :
    • Amidophosphoribosyltransferase (EC 2.4.2.14)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Amidophosphoribosyltransferase (EC 2.4.2.14)
    and 1 more
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Amidophosphoribosyltransferase (EC 2.4.2.14)
  • PFAM:
    NTN (CL0052) GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 71.9)
    GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 66.3)
    and 4 more
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 40.6)
    NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 22.3)
    PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 16.4)
    PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003038
    • TAT(Tat/SPI): 0.000224
    • LIPO(Sec/SPII): 0.000463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 471 [4]
  • interaction partners:
    SACOL0211acetyl-CoA acetyltransferase  [5] (data from MRSA252)
    SACOL02123-hydroxyacyl-CoA dehydrogenase  [5] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ 5.0 5.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL1079&oldid=92055"