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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001029
  • pan locus tag?: SAUPAN003277000
  • symbol: purE
  • pan gene symbol?: purE
  • synonym:
  • product: 5-(carboxyamino)imidazole ribonucleotide mutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001029
  • symbol: purE
  • product: 5-(carboxyamino)imidazole ribonucleotide mutase
  • replicon: chromosome
  • strand: +
  • coordinates: 1033097..1033579
  • length: 483
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    GTGAAAGTAGCAGTCATTATGGGCAGTTCTTCCGATTGGAAAATTATGCAAGAGAGTTGT
    AACATGTTGGATTATTTTGAAATTCCGTACGAAAAACAAGTAGTATCCGCACATCGTACG
    CCAAAAATGATGGTTCAATTTGCTTCTGAAGCGAGAGAAAGAGGTATAAACATTATCATT
    GCAGGCGCTGGCGGTGCGGCACATTTACCAGGTATGGTTGCATCATTAACGACGCTACCA
    GTTATTGGAGTGCCGATTGAAACAAAAAGTTTAAAGGGTATAGATTCTTTATTATCAATT
    GTTCAAATGCCAGGAGGTATTCCGGTTGCAACGACTGCAATTGGTGCAGCAGGTGCTAAA
    AACGCAGGTATACTTGCAGCAAGAATGTTAAGTATTCAAAATCCTTCTTTAGTTGAAAAA
    CTAAATCAGTATGAATCTTCGTTAATTCAAAAAGTGGAGGACATGCAAAATGAACTTCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    483

⊟Protein[edit | edit source]

Protein Data Bank: 3ORS

⊟General[edit | edit source]

  • locus tag: JSNZ_001029
  • symbol: PurE
  • description: 5-(carboxyamino)imidazole ribonucleotide mutase
  • length: 160
  • theoretical pI: 6.52439
  • theoretical MW: 17068.9
  • GRAVY: 0.189375

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.21?  ExPASy
    Phosphoribosylaminoimidazole carboxylase 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2
    EC 5.4.99.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide mutase 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, catalytic subunit (TIGR01162; EC 4.1.1.21; HMM-score: 217.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    DHQS (CL0224) AIRC; AIR carboxylase (PF00731; HMM-score: 215.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9141
    • Cytoplasmic Membrane Score: 0.0056
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0798
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018469
    • TAT(Tat/SPI): 0.000661
    • LIPO(Sec/SPII): 0.007281
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMMVQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQMPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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