⊟Summary[edit | edit source]

PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
pan locus tag^?: SAUPAN001446000
symbol: SACOL0343
pan gene symbol^?: —
• synonym:
• product: prophage L54a, replicative DNA helicase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
• symbol: SACOL0343
• product: prophage L54a, replicative DNA helicase
• replicon: chromosome
• strand: +
• coordinates: 365904..367145
• length: 1242
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236905 NCBI
• RefSeq: YP_185235 NCBI
• BioCyc: see SACOL_RS01725
• MicrobesOnline: 911814 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAATTATGGACAATTTGAAATTGAAAGCACAATAATCGCTACGCTGCTCAAACAACCG
  GACGTACTAGAAAAGATACGAGTTAAAGATTATATGTTTACGAACGAAAAGTTTAAAACC
  TTTTTCAATTATGTAATGGACGTCGGAAAGATAGATCATCAAGAAATTTATTTAAAAGCA
  ACTAAAGATAAAGAATTTTTAGATGCAGATACTATAACTAAACTTTATAACTCCGATTTC
  ATTGGATACGGTTTCTTTGAACGTTATCAACAAGAATTATTGGAAAGTTATCAACTCAAC
  AAAGCTAACGAATTGGTAACTGAGTTCAAACAACAACCTACGAATCAAAACTTTAACAAC
  TTGATTGATGAACTCAAGGATTTAAAAACGATTACTAACAAAAAAGAAGATGGAACCAAG
  AAGTTTGTTGAGGAGTTTGTCGAAGAGTTATACAGCGATAGCCCTAAGAAGCAAATTAAG
  ACGGGTTACAAGCTAATGGATTACAAAATAGGGGGATTAGAGCCATCACAATTAATCGTC
  ATCGCAGCGCGTCCCTCAGTGGGTAAGACAGGCTTTGCATTAAACATGATGCTGAACATA
  GCACAAAATGGATACAAAACATCTTTCTTTAGTCTCGAAACAACCGGCACATCGGTATTG
  AAACGTATGTTATCAACAATTACTGGTATTGAGTTAACAAAGATAAAAGAAATCAGGAAC
  TTAACGCCGGATGACTTAACAAAGTTAACGAATGCGATGGATAAAATCATGAAATTAGGC
  ATCGATATTTCTGATAAAAGTAATATCACACCGCAAGATGTGCGAGCGCAAGCAATGAGG
  CATTCAGACGGGCAACAAGTTATTTTTATAGATTACCTTCAACTGATGGATACTGATGCG
  AAAGTTGATAGACGTGTAGCAGTAGAAAAGATATCACGCGACTTAAAGATAATCGCTAAC
  GAGACAGGCGCAATCATTGTACTACTTTCACAACTGAATCGTGGAGTCGAGTCTAGGCAG
  GATAAACGACCAATGCTATCGGACATGAAAGAATCAGGCGGAATAGAGGCAGATGCGAGT
  TTAGCAATGCTACTTTACCGTGATGATTATTATAACCGTGACGAAGATGACAGTATCACA
  GGCAAATCTATTGTTGAATGTAACATAGCCAAAAACAAAGACGGCGAAACCGGAATAATT
  GAATTCGAGTATTACAAGAAGACGCAGAGGTTTTTCACATGA
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⊟Protein

⊟General

• locus tag: SACOL0343 [new locus tag: SACOL_RS01725 ]
• symbol: SACOL0343
• description: prophage L54a, replicative DNA helicase
• length: 413
• theoretical pI: 5.0803
• theoretical MW: 47460.9
• GRAVY: -0.492736

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 272.6)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 235.4)
  and 3 more
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 19.2)
  KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 19.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RadA (TIGR00416; HMM-score: 15.8)
• TheSEED  :
  • DNA helicase, phage-associated
• ⊞⊞PFAM:
  P-loop_NTPase (CL0023) DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 252.4)
  and 7 more
  AAA_25; AAA domain (PF13481; HMM-score: 30)
  ATPase; KaiC (PF06745; HMM-score: 28.3)
  divDNAB; Divergent DnaB-like ATPase (PF20307; HMM-score: 16)
  Phage_TACs (CL0567) Gp41_Mu; Tail assembly protein gp41 (PF23746; HMM-score: 12.8)
  P-loop_NTPase (CL0023) ParA; NUBPL iron-transfer P-loop NTPase (PF10609; HMM-score: 12.7)
  no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 11.8)
  P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.6523
  • Cytoplasmic Membrane Score: 0.1442
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.203
• ⊞⊞LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00157
  • TAT(Tat/SPI): 0.000077
  • LIPO(Sec/SPII): 0.000291
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651270 NCBI
• RefSeq: YP_185235 NCBI
• UniProt: A0A0H2WYA4 UniProt

⊟Protein sequence

• MNYGQFEIESTIIATLLKQPDVLEKIRVKDYMFTNEKFKTFFNYVMDVGKIDHQEIYLKATKDKEFLDADTITKLYNSDFIGYGFFERYQQELLESYQLNKANELVTEFKQQPTNQNFNNLIDELKDLKTITNKKEDGTKKFVEEFVEELYSDSPKKQIKTGYKLMDYKIGGLEPSQLIVIAARPSVGKTGFALNMMLNIAQNGYKTSFFSLETTGTSVLKRMLSTITGIELTKIKEIRNLTPDDLTKLTNAMDKIMKLGIDISDKSNITPQDVRAQAMRHSDGQQVIFIDYLQLMDTDAKVDRRVAVEKISRDLKIIANETGAIIVLLSQLNRGVESRQDKRPMLSDMKESGGIEADASLAMLLYRDDYYNRDEDDSITGKSIVECNIAKNKDGETGIIEFEYYKKTQRFFT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: Cytoplasmic
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0335 > SACOL0336 > SACOL0337 > SACOL0338 > ssb1 > SACOL0340 > SACOL0341 > SACOL0342 > SACOL0343 > SACOL0344 > SACOL0345 > SACOL0346 > SACOL0347 > SACOL0348 > SACOL0349 > SACOL0350 > SACOL0351 > SACOL0352 > SACOL0353 > SACOL0354 > SACOL0355 > SACOL0356 > dut

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications