PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0349 [new locus tag: SACOL_RS01755 ]
• pan locus tag^?: SAUPAN001453000
• symbol: SACOL0349
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0349 [new locus tag: SACOL_RS01755 ]
• symbol: SACOL0349
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 368608..368865
• length: 258
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236911 NCBI
• RefSeq: YP_185241 NCBI
• BioCyc: see SACOL_RS01755
• MicrobesOnline: 911820 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  
  ATGAAGTTGAACGAAGTATTCGCAACTAATTTAAGAGTAATCATGGCTAGAGATAACGTA
  AGTGTTCAAGATTTGCACAATGAAACTGGCGTATCAAGATCAACTATTAGTGGATATAAA
  AACGGAAAAGCTGAGATGGTTAACTTAAATGTATTAGATAAATTGGCAGATGCTCTAGGT
  GTTAATGTAAGTGAACTATTTACTAGAAATCACAACACGCACAAATTAGAGGATTGGATT
  AAAAAAGTAAATGTATAG
  60
  120
  180
  240
  258
  

⊟Protein

⊟General

• locus tag: SACOL0349 [new locus tag: SACOL_RS01755 ]
• symbol: SACOL0349
• description: hypothetical protein
• length: 85
• theoretical pI: 9.11945
• theoretical MW: 9565.85
• GRAVY: -0.341176

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 15.1)
• TheSEED  :
  • Phage transcriptional regulator cro
• PFAM:
  HTH (CL0123) HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 59.8)
  and 14 more
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 34.9)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 32.2)
  HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 31.6)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 22.5)
  HTH_11; HTH domain (PF08279; HMM-score: 20.1)
  TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 16.7)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 15.1)
  Met_repress (CL0057) RHH_1; Ribbon-helix-helix protein, copG family (PF01402; HMM-score: 14.6)
  HTH (CL0123) HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 14.4)
  MarR_2; MarR family (PF12802; HMM-score: 13.1)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.9)
  MarR; MarR family (PF01047; HMM-score: 12.6)
  Phage_CI_repr; Bacteriophage CI repressor helix-turn-helix domain (PF07022; HMM-score: 12.4)
  Met_repress (CL0057) RHH_3; Ribbon-helix-helix domain (PF12651; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00715
  • TAT(Tat/SPI): 0.000363
  • LIPO(Sec/SPII): 0.000844
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651276 NCBI
• RefSeq: YP_185241 NCBI
• UniProt: A0A0H2WY08 UniProt

⊟Protein sequence

• MKLNEVFATNLRVIMARDNVSVQDLHNETGVSRSTISGYKNGKAEMVNLNVLDKLADALGVNVSELFTRNHNTHKLEDWIKKVNV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0335 > SACOL0336 > SACOL0337 > SACOL0338 > ssb1 > SACOL0340 > SACOL0341 > SACOL0342 > SACOL0343 > SACOL0344 > SACOL0345 > SACOL0346 > SACOL0347 > SACOL0348 > SACOL0349 > SACOL0350 > SACOL0351 > SACOL0352 > SACOL0353 > SACOL0354 > SACOL0355 > SACOL0356 > dut

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications