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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
- pan locus tag?: SAUPAN001449000
- symbol: SACOL0346
- pan gene symbol?: —
- synonym:
- product: prophage L54a, N-6-adenine-methyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
- symbol: SACOL0346
- product: prophage L54a, N-6-adenine-methyltransferase
- replicon: chromosome
- strand: +
- coordinates: 367591..368016
- length: 426
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3236908 NCBI
- RefSeq: YP_185238 NCBI
- BioCyc: see SACOL_RS01740
- MicrobesOnline: 911817 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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421ATGGAAGTACATTACAGTAGTAAAACAAACGAGTGGACAACACCACAACATTTATTTGAT
GACCTAAGCGAAGAATTCAGTTTTACATTAGATCCTTGTTCAACAGACGAGAACGCCAAA
TGCCGGAAGTATTATACAGTAAAAGATAATGGGTTAATTCAAGACTGGTCTGAGGACATT
GTTTTTATGAACCCGCCATACGGTCGAAGTATTAAGCGTTGGGTCAAGAAGGCTTACGAA
GAAAGTTTGAAAGGCGCAACGGTAGTTTGTTTAATACCCGCAAGAACAGACACGACATAT
TGGCATGATTACATTTTTAATAAGGCTGATGATATAAGATTCCTACGCGGTCGTCTGAAG
TTTGGAGATAGTAAAAACAGCGCTCCTTTTCCTAGCGCAATTATCGTTTATAGAGGTGCA
CAATGA60
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426
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0346 [new locus tag: SACOL_RS01740 ]
- symbol: SACOL0346
- description: prophage L54a, N-6-adenine-methyltransferase
- length: 141
- theoretical pI: 6.77433
- theoretical MW: 16435.4
- GRAVY: -0.634752
⊟Function[edit | edit source]
- reaction: EC 2.1.1.72? ExPASySite-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
- TIGRFAM: Mobile and extrachromosomal element functions Prophage functions phage N-6-adenine-methyltransferase (TIGR01712; EC 2.1.1.72; HMM-score: 96.4)DNA metabolism Restriction/modification phage N-6-adenine-methyltransferase (TIGR01712; EC 2.1.1.72; HMM-score: 96.4)
- TheSEED:
- PFAM: no clan defined Dam; DNA N-6-adenine-methyltransferase (Dam) (PF05869; HMM-score: 112.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.009647
- TAT(Tat/SPI): 0.000654
- LIPO(Sec/SPII): 0.002236
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MEVHYSSKTNEWTTPQHLFDDLSEEFSFTLDPCSTDENAKCRKYYTVKDNGLIQDWSEDIVFMNPPYGRSIKRWVKKAYEESLKGATVVCLIPARTDTTYWHDYIFNKADDIRFLRGRLKFGDSKNSAPFPSAIIVYRGAQ
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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