PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01725 [old locus tag: SACOL0343 ]
• pan locus tag^?: SAUPAN001446000
• symbol: SACOL_RS01725
• pan gene symbol^?: —
• synonym:
• product: DNA damage-inducible protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01725 [old locus tag: SACOL0343 ]
• symbol: SACOL_RS01725
• product: DNA damage-inducible protein
• replicon: chromosome
• strand: +
• coordinates: 365904..367145
• length: 1242
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (365904..367145) NCBI
• BioCyc: SACOL_RS01725 BioCyc
• MicrobesOnline: see SACOL0343

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATTATGGACAATTTGAAATTGAAAGCACAATAATCGCTACGCTGCTCAAACAACCG
  GACGTACTAGAAAAGATACGAGTTAAAGATTATATGTTTACGAACGAAAAGTTTAAAACC
  TTTTTCAATTATGTAATGGACGTCGGAAAGATAGATCATCAAGAAATTTATTTAAAAGCA
  ACTAAAGATAAAGAATTTTTAGATGCAGATACTATAACTAAACTTTATAACTCCGATTTC
  ATTGGATACGGTTTCTTTGAACGTTATCAACAAGAATTATTGGAAAGTTATCAACTCAAC
  AAAGCTAACGAATTGGTAACTGAGTTCAAACAACAACCTACGAATCAAAACTTTAACAAC
  TTGATTGATGAACTCAAGGATTTAAAAACGATTACTAACAAAAAAGAAGATGGAACCAAG
  AAGTTTGTTGAGGAGTTTGTCGAAGAGTTATACAGCGATAGCCCTAAGAAGCAAATTAAG
  ACGGGTTACAAGCTAATGGATTACAAAATAGGGGGATTAGAGCCATCACAATTAATCGTC
  ATCGCAGCGCGTCCCTCAGTGGGTAAGACAGGCTTTGCATTAAACATGATGCTGAACATA
  GCACAAAATGGATACAAAACATCTTTCTTTAGTCTCGAAACAACCGGCACATCGGTATTG
  AAACGTATGTTATCAACAATTACTGGTATTGAGTTAACAAAGATAAAAGAAATCAGGAAC
  TTAACGCCGGATGACTTAACAAAGTTAACGAATGCGATGGATAAAATCATGAAATTAGGC
  ATCGATATTTCTGATAAAAGTAATATCACACCGCAAGATGTGCGAGCGCAAGCAATGAGG
  CATTCAGACGGGCAACAAGTTATTTTTATAGATTACCTTCAACTGATGGATACTGATGCG
  AAAGTTGATAGACGTGTAGCAGTAGAAAAGATATCACGCGACTTAAAGATAATCGCTAAC
  GAGACAGGCGCAATCATTGTACTACTTTCACAACTGAATCGTGGAGTCGAGTCTAGGCAG
  GATAAACGACCAATGCTATCGGACATGAAAGAATCAGGCGGAATAGAGGCAGATGCGAGT
  TTAGCAATGCTACTTTACCGTGATGATTATTATAACCGTGACGAAGATGACAGTATCACA
  GGCAAATCTATTGTTGAATGTAACATAGCCAAAAACAAAGACGGCGAAACCGGAATAATT
  GAATTCGAGTATTACAAGAAGACGCAGAGGTTTTTCACATGA
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⊟Protein

⊟General

• locus tag: SACOL_RS01725 [old locus tag: SACOL0343 ]
• symbol: SACOL_RS01725
• description: DNA damage-inducible protein
• length: 413
• theoretical pI: 5.0803
• theoretical MW: 47460.9
• GRAVY: -0.492736

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 272.6)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 235.4)
  and 3 more
  KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 19.2)
  KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 19.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein RadA (TIGR00416; HMM-score: 15.8)
• TheSEED: see SACOL0343
• PFAM:
  P-loop_NTPase (CL0023) DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 249.9)
  and 3 more
  AAA_25; AAA domain (PF13481; HMM-score: 34.8)
  no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 13)
  HAD (CL0137) Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00157
  • TAT(Tat/SPI): 0.000077
  • LIPO(Sec/SPII): 0.000291
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447028912 NCBI
• RefSeq: WP_001106168 NCBI
• UniProt: see SACOL0343

⊟Protein sequence

• MNYGQFEIESTIIATLLKQPDVLEKIRVKDYMFTNEKFKTFFNYVMDVGKIDHQEIYLKATKDKEFLDADTITKLYNSDFIGYGFFERYQQELLESYQLNKANELVTEFKQQPTNQNFNNLIDELKDLKTITNKKEDGTKKFVEEFVEELYSDSPKKQIKTGYKLMDYKIGGLEPSQLIVIAARPSVGKTGFALNMMLNIAQNGYKTSFFSLETTGTSVLKRMLSTITGIELTKIKEIRNLTPDDLTKLTNAMDKIMKLGIDISDKSNITPQDVRAQAMRHSDGQQVIFIDYLQLMDTDAKVDRRVAVEKISRDLKIIANETGAIIVLLSQLNRGVESRQDKRPMLSDMKESGGIEADASLAMLLYRDDYYNRDEDDSITGKSIVECNIAKNKDGETGIIEFEYYKKTQRFFT

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: see SACOL0343
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications