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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0341 [new locus tag: SACOL_RS01715 ]
  • pan locus tag?: SAUPAN001444000
  • symbol: SACOL0341
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0341 [new locus tag: SACOL_RS01715 ]
  • symbol: SACOL0341
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 364751..365551
  • length: 801
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGGAGAAGTATCGTGGATAAAACTTAAAGTTGGCATGTTTGATGACAGCAAAATCAAA
    TATATCGAAGCTTTACCCGAAAGAGATACGATCATAACTATTTGGGTTAAGTTGCTAACT
    TTATCAGGAAAGTACAATGAACAAGGTTATATTATGCTATCCGAAAACTTGCCGTACAAC
    GAAGAAATGTTAGCAAATGAATTTAATAGACCTATTAACTCAATAAGGTTAGCAATTCAA
    ACTTTTGAGACATTGGGCATGATTGAAAAAGTTAATGGTGTCATAAAAGTGACAAACTGG
    GAGAAGCATCAAAGCTTAGATAGCAAAGCTAAGCATAAAGAAAAAAATAAATTGCGACAA
    CAACGCTATCGAGAGAGACAGAAAAAGTTACTAGAAGCAAAACGTAACGTTACCGTAACG
    TTACGTAACGATACAGAAGAAGAAAGAGAAGAAGAAAAAGAAGAAGAATATAAGAATAAA
    GAAGAAGAAAGAGAAGCCGTCTTCTCATCTTCAATAAAATATATAATTGCAAATTTGGAT
    GATAAGTTAACGCCTAATCAAATGGAGCAATTAGGGTTTGCTATTGATGATATAGGTACG
    AACGCTTTTGAAGTTGTAAAAGTAGGTGTTGAGTACACTAAAAGCAAAAGTGCGCATGGT
    GGCTACTTAATTAAAGTTTTGAACAACTGGGCTAAAGAGAATGTCAAAACAAAAGAAGAT
    GCAGAACACAAAATAGCGCCTAGGAAAAACACAACTGATGATGTCATTGCGCAAATGGAA
    AAAGAATTGAGTGATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0341 [new locus tag: SACOL_RS01715 ]
  • symbol: SACOL0341
  • description: hypothetical protein
  • length: 266
  • theoretical pI: 5.57701
  • theoretical MW: 30991
  • GRAVY: -0.817293

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replisome organizer, putative, N-terminal region (TIGR01714; HMM-score: 177.3)
    and 3 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 62.3)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 62.3)
    Metabolism Energy metabolism Sugars L-fucose isomerase (TIGR01089; EC 5.3.1.25; HMM-score: 12.2)
  • TheSEED  :
    • Phage replication initiation
  • ⊞PFAM:
    HTH (CL0123) Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 177.4)
    and 7 more
    no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 40.4)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.8)
    no clan defined CSRNP_N; Cysteine/serine-rich nuclear protein N-terminus (PF16019; HMM-score: 12.3)
    Tbcl_zf (CL0839) DUF7564; Family of unknown function (DUF7564) (PF24445; HMM-score: 10.6)
    no clan defined Piezo_TM25-28; Piezo TM25-28 (PF15917; HMM-score: 9.9)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 8.7)
    no clan defined PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9762
    • Cytoplasmic Membrane Score: 0.0036
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0188
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002556
    • TAT(Tat/SPI): 0.000226
    • LIPO(Sec/SPII): 0.000398
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGKYNEQGYIMLSENLPYNEEMLANEFNRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEKHQSLDSKAKHKEKNKLRQQRYRERQKKLLEAKRNVTVTLRNDTEEEREEEKEEEYKNKEEEREAVFSSSIKYIIANLDDKLTPNQMEQLGFAIDDIGTNAFEVVKVGVEYTKSKSAHGGYLIKVLNNWAKENVKTKEDAEHKIAPRKNTTDDVIAQMEKELSDD

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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