PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01715 [old locus tag: SACOL0341 ]
• pan locus tag^?: SAUPAN001444000
• symbol: SACOL_RS01715
• pan gene symbol^?: —
• synonym:
• product: replication protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01715 [old locus tag: SACOL0341 ]
• symbol: SACOL_RS01715
• product: replication protein
• replicon: chromosome
• strand: +
• coordinates: 364751..365551
• length: 801
• essential: unknown

⊟Accession numbers

• Location: NC_002951 (364751..365551) NCBI
• BioCyc: SACOL_RS01715 BioCyc
• MicrobesOnline: see SACOL0341

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGAGAAGTATCGTGGATAAAACTTAAAGTTGGCATGTTTGATGACAGCAAAATCAAA
  TATATCGAAGCTTTACCCGAAAGAGATACGATCATAACTATTTGGGTTAAGTTGCTAACT
  TTATCAGGAAAGTACAATGAACAAGGTTATATTATGCTATCCGAAAACTTGCCGTACAAC
  GAAGAAATGTTAGCAAATGAATTTAATAGACCTATTAACTCAATAAGGTTAGCAATTCAA
  ACTTTTGAGACATTGGGCATGATTGAAAAAGTTAATGGTGTCATAAAAGTGACAAACTGG
  GAGAAGCATCAAAGCTTAGATAGCAAAGCTAAGCATAAAGAAAAAAATAAATTGCGACAA
  CAACGCTATCGAGAGAGACAGAAAAAGTTACTAGAAGCAAAACGTAACGTTACCGTAACG
  TTACGTAACGATACAGAAGAAGAAAGAGAAGAAGAAAAAGAAGAAGAATATAAGAATAAA
  GAAGAAGAAAGAGAAGCCGTCTTCTCATCTTCAATAAAATATATAATTGCAAATTTGGAT
  GATAAGTTAACGCCTAATCAAATGGAGCAATTAGGGTTTGCTATTGATGATATAGGTACG
  AACGCTTTTGAAGTTGTAAAAGTAGGTGTTGAGTACACTAAAAGCAAAAGTGCGCATGGT
  GGCTACTTAATTAAAGTTTTGAACAACTGGGCTAAAGAGAATGTCAAAACAAAAGAAGAT
  GCAGAACACAAAATAGCGCCTAGGAAAAACACAACTGATGATGTCATTGCGCAAATGGAA
  AAAGAATTGAGTGATGACTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS01715 [old locus tag: SACOL0341 ]
• symbol: SACOL_RS01715
• description: replication protein
• length: 266
• theoretical pI: 5.57701
• theoretical MW: 30991
• GRAVY: -0.817293

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replisome organizer, putative, N-terminal region (TIGR01714; HMM-score: 177.3)
  and 3 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 62.3)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 62.3)
  Metabolism Energy metabolism Sugars L-fucose isomerase (TIGR01089; EC 5.3.1.25; HMM-score: 12.2)
• TheSEED: see SACOL0341
• PFAM:
  HTH (CL0123) Phage_rep_org_N; N-terminal phage replisome organiser (Phage_rep_org_N) (PF09681; HMM-score: 161.2)
  and 4 more
  no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 44.1)
  ssDNA-exonuc_C; Single-strand DNA-specific exonuclease, C terminal domain (PF10141; HMM-score: 14.7)
  CSRNP_N; Cysteine/serine-rich nuclear protein N-terminus (PF16019; HMM-score: 12.5)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002556
  • TAT(Tat/SPI): 0.000226
  • LIPO(Sec/SPII): 0.000398
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446427121 NCBI
• RefSeq: WP_000504976 NCBI
• UniProt: see SACOL0341

⊟Protein sequence

• MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGKYNEQGYIMLSENLPYNEEMLANEFNRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEKHQSLDSKAKHKEKNKLRQQRYRERQKKLLEAKRNVTVTLRNDTEEEREEEKEEEYKNKEEEREAVFSSSIKYIIANLDDKLTPNQMEQLGFAIDDIGTNAFEVVKVGVEYTKSKSAHGGYLIKVLNNWAKENVKTKEDAEHKIAPRKNTTDDVIAQMEKELSDD

⊟Experimental data

• experimentally validated:
• protein localization: see SACOL0341
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications