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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01205
  • pan locus tag?: SAUPAN003522000
  • symbol: SAOUHSC_01205
  • pan gene symbol?: ftsY
  • synonym:
  • product: signal recognition particle-docking protein FtsY

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01205
  • symbol: SAOUHSC_01205
  • product: signal recognition particle-docking protein FtsY
  • replicon: chromosome
  • strand: +
  • coordinates: 1156338..1157588
  • length: 1251
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTT
    AAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCA
    ACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTG
    AGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAA
    GCTCAAAAAATGGGTGCTAAATTTAAAGCAGGACTCGAAAAATCTCGTCAAAATTTCCAA
    GAACAATTAAATAATTTGATAGCGAGATATCGTAAAGTAGATGAAGACTTTTTTGAAGCT
    TTAGAAGAAATGTTAATCACTGCAGACGTCGGTTTTAATACAGTGATGACGTTAACTGAA
    GAATTACGTATGGAAGCACAACGACGTAATATTCAAGATACTGAAGATTTGCGTGAAGTC
    ATTGTTGAAAAGATCGTAGAGATTTACCATCAAGAAGATGATAATTCAGAAGCTATGAAC
    TTAGAAGATGGTCGTTTAAATGTCATTTTAATGGTTGGTGTGAATGGTGTTGGTAAAACA
    ACAACAATTGGAAAATTAGCTTACCGATATAAAATGGAAGGTAAAAAAGTAATGTTAGCT
    GCGGGCGATACTTTTAGAGCGGGTGCTATTGATCAATTGAAAGTTTGGGGCGAACGTGTT
    GGTGTAGACGTAATTAGCCAAAGTGAAGGTTCTGATCCAGCTGCTGTTATGTATGATGCG
    ATTAATGCCGCTAAAAACAAAGGTGTTGATATTTTAATCTGTGATACCGCTGGACGTTTA
    CAAAATAAAACAAATCTAATGCAAGAATTAGAAAAAGTTAAGCGTGTAATTAATCGAGCA
    GTGCCAGATGCGCCTCATGAAGCATTACTATGTTTAGATGCTACAACTGGTCAGAATGCG
    TTGTCACAAGCTAGAAACTTTAAAGAAGTAACAAATGTTACAGGTATTGTATTAACGAAA
    TTAGATGGTACAGCCAAAGGTGGTATCGTATTAGCCATTCGTAATGAATTGCACATCCCA
    GTTAAATATGTAGGTTTAGGTGAGCAATTAGATGACTTACAACCATTTAACCCTGAAAGT
    TATGTCTACGGCTTATTCGCTGATATGATTGAACAAAATGAAGAAATAACAACAGTTGAA
    AATGATCAAATTGTAACAGAAGAAAAGGACGATAATCATGGGTCAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01205
  • symbol: SAOUHSC_01205
  • description: signal recognition particle-docking protein FtsY
  • length: 416
  • theoretical pI: 4.39014
  • theoretical MW: 46590
  • GRAVY: -0.545673

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 388.5)
    and 11 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle protein (TIGR00959; HMM-score: 233.6)
    signal recognition particle protein SRP54 (TIGR01425; HMM-score: 185.8)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 91.4)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 18.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16)
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 15.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 13.2)
    Unknown function General putative selenium-dependent hydroxylase accessory protein YqeC (TIGR03172; HMM-score: 12.9)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 12)
  • ⊞TheSEED  :
    • Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
    and 1 more
    Protein Metabolism Protein biosynthesis Universal GTPases  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
  • ⊞PFAM:
    P-loop_NTPase (CL0023) SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 272.1)
    and 20 more
    no clan defined SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 51)
    P-loop_NTPase (CL0023) AAA_30; AAA domain (PF13604; HMM-score: 19)
    nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 18.7)
    AAA_22; AAA domain (PF13401; HMM-score: 18.5)
    MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 18.4)
    ABC_tran; ABC transporter (PF00005; HMM-score: 17.1)
    AAA_17; AAA domain (PF13207; HMM-score: 16.4)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 16.1)
    AAA_31; AAA domain (PF13614; HMM-score: 15.7)
    AAA_33; AAA domain (PF13671; HMM-score: 15.1)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.4)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 13.9)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.7)
    no clan defined Scm3; Centromere protein Scm3 (PF10384; HMM-score: 13.2)
    P-loop_NTPase (CL0023) APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 13.1)
    AAA_24; AAA domain (PF13479; HMM-score: 13)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.6)
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 11.5)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 10.7)
    YqeC; YqeC protein (PF19842; HMM-score: 9.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.6981
    • Cytoplasmic Membrane Score: 0.2663
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0349
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007941
    • TAT(Tat/SPI): 0.001861
    • LIPO(Sec/SPII): 0.0026
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDANDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQLNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREVIVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVMLAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDTAGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVTGIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQNEEITTVENDQIVTEEKDDNHGSK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ Jump up to: 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ Jump up to: 5.0 5.1 5.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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