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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1078 [new locus tag: SA_RS06110 ]
  • pan locus tag?: SAUPAN003522000
  • symbol: SA1078
  • pan gene symbol?: ftsY
  • synonym:
  • product: signal recognition particle protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1078 [new locus tag: SA_RS06110 ]
  • symbol: SA1078
  • product: signal recognition particle protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1220711..1221961
  • length: 1251
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTT
    AAATCCTTAAGAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCA
    ACGCAGGACGTAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTG
    AGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAA
    GCTCAAAAAATGGGTGCTAAATTTAAAGCAGGACTCGAAAAATCTCGTCAAAATTTCCAA
    GAACAATTAAATAATTTGATAGCGAGATATCGTAAAGTAGATGAAGACTTTTTTGAAGCT
    TTAGAAGAAATGTTAATCACTGCAGACGTCGGTTTTAATACAGTGATGACGTTAACTGAA
    GAATTACGTATGGAAGCACAACGACGTAATATTCAAGATACTGAAGATTTGCGTGAAGTC
    ATTGTTGAAAAAATCGTAGAGATTTACCATCAAGAAGATGATAATTCAGAAGCTATGAAC
    ATAGAAGATGGTCGTTTAAATGTCATTTTAATGGTCGGTGTGAATGGTGTTGGTAAAACA
    ACAACAATTGGAAAATTAGCTTACCGATATAAAATGGAAGGTAAAAAAGTAATGTTAGCT
    GCGGGCGATACTTTTAGAGCGGGTGCTATTGATCAATTGAAAGTTTGGGGCGAACGTGTT
    GGTGTAGATGTAATTAGCCAAAGTGAAGGTTCTGATCCAGCTGCTGTTATGTATGATGCG
    ATTAATGCCGCTAAAAACAAAGGTGTTGATATTTTAATCTGTGATACCGCTGGACGTTTA
    CAAAATAAAACAAATCTAATGCAAGAATTAGAAAAAGTTAAGCGTGTAATTAATCGAGCA
    GTGCCAGATGCGCCTCATGAAGCATTACTATGTTTAGATGCTACAACTGGTCAGAATGCG
    TTGTCACAAGCTAGAAACTTTAAAGAAGTAACAAATGTTACAGGTATTGTATTAACGAAA
    TTAGATGGTACAGCCAAAGGTGGTATCGTATTAGCCATTCGTAATGAATTGCACATTCCA
    GTTAAATATGTAGGTTTAGGTGAGCAATTAGATGACTTACAACCATTTAACCCTGAAAGT
    TATGTCTACGGCTTATTCGCTGATATGATTGAACAAAATGACGAAATAACAACAGTTGAA
    AATGATCAAATTGTAACAGAAGAAAAGGACGATAATCATGGGGCAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1078 [new locus tag: SA_RS06110 ]
  • symbol: SA1078
  • description: signal recognition particle protein
  • length: 416
  • theoretical pI: 4.41395
  • theoretical MW: 46643.1
  • GRAVY: -0.541106

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 388.4)
    and 9 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle protein (TIGR00959; HMM-score: 234)
    signal recognition particle protein SRP54 (TIGR01425; HMM-score: 184.7)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 90.7)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 18.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.8)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.8)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16.4)
    Unknown function General putative selenium-dependent hydroxylase accessory protein YqeC (TIGR03172; HMM-score: 12.8)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 11.5)
  • TheSEED  :
    • Signal recognition particle receptor FtsY
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
    and 1 more
    Protein Metabolism Protein biosynthesis Universal GTPases  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
  • PFAM:
    P-loop_NTPase (CL0023) SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 273.6)
    and 18 more
    no clan defined SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 48.9)
    P-loop_NTPase (CL0023) ArgK; ArgK protein (PF03308; HMM-score: 18.7)
    AAA_30; AAA domain (PF13604; HMM-score: 18.7)
    AAA_17; AAA domain (PF13207; HMM-score: 16.9)
    ABC_tran; ABC transporter (PF00005; HMM-score: 15.4)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 15.2)
    AAA_31; AAA domain (PF13614; HMM-score: 15.2)
    AAA_22; AAA domain (PF13401; HMM-score: 15)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.8)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.5)
    AAA_33; AAA domain (PF13671; HMM-score: 14.2)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.3)
    AAA_24; AAA domain (PF13479; HMM-score: 13.2)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 12.8)
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 11.1)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 9.5)
    no clan defined Scm3; Centromere protein Scm3 (PF10384; HMM-score: 6.2)
    P-loop_NTPase (CL0023) AAA_32; AAA domain (PF13654; HMM-score: 4.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00662
    • TAT(Tat/SPI): 0.001643
    • LIPO(Sec/SPII): 0.002106
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFFKRLKDKFATNKENEEVKSLREEQGQDKLEDTHSEGSTQDVNDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQLNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREVIVEKIVEIYHQEDDNSEAMNIEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVMLAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDTAGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVTGIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQNDEITTVENDQIVTEEKDDNHGAK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA0033(aadD)kanamycin nucleotidyltransferase  [2] (data from MRSA252)
    SA0366(ahpC)alkyl hydroperoxide reductase  [2] (data from MRSA252)
    SA1517(citC)isocitrate dehydrogenase  [2] (data from MRSA252)
    SA0731(eno)phosphopyruvate hydratase  [2] (data from MRSA252)
    SA1510(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [2] (data from MRSA252)
    SA1150(glnA)glutamine-ammonia ligase  [2] (data from MRSA252)
    SA2334(mvaS)3-hydroxy-3-methylglutaryl-CoA synthase  [2] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [2] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [2] (data from MRSA252)
    SA1245(sucA)2-oxoglutarate dehydrogenase E1  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA1914(upp)uracil phosphoribosyltransferase  [2] (data from MRSA252)
    SA2327pyruvate oxidase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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