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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1128 [new locus tag: SAUSA300_RS06105 ]
  • pan locus tag?: SAUPAN003522000
  • symbol: ftsY
  • pan gene symbol?: ftsY
  • synonym:
  • product: signal recognition particle-docking protein FtsY

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1128 [new locus tag: SAUSA300_RS06105 ]
  • symbol: ftsY
  • product: signal recognition particle-docking protein FtsY
  • replicon: chromosome
  • strand: +
  • coordinates: 1236284..1237534
  • length: 1251
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGCTTTTTTAAACGCTTAAAAGATAAGTTTGCAACAAATAAAGAAAATGAAGAAGTT
    AAATCCTTAACAGAAGAACAAGGTCAAGACAAATTAGAAGATACACATTCTGAAGGTTCA
    ACGCAGGACGCAAATGATTTAGCAGAAAATGCTGAAGTGAAAAAGAAGCCACGCAAGTTG
    AGTGAAGCGGATTTTGATGACGATGGCTTAATATCAATTGAAGATTTTGAAGAAATTGAA
    GCTCAAAAAATGGGTGCTAAATTTAAAGCAGGACTCGAAAAATCTCGTCAAAATTTCCAA
    GAACAATTAAATAATTTGATAGCGAGATATCGTAAAGTAGATGAAGACTTTTTTGAAGCT
    TTAGAAGAAATGTTAATCACTGCAGACGTCGGTTTTAATACAGTGATGACGTTAACTGAA
    GAATTACGTATGGAAGCACAACGACGTAATATTCAAGATACTGAAGATTTGCGTGAAGTC
    ATTGTTGAAAAAATCGTAGAGATTTACCATCAAGAAGATGATAATTCAGAAGCTATGAAC
    TTAGAAGATGGTCGTTTAAATGTCATTTTAATGGTTGGTGTGAATGGTGTTGGTAAAACA
    ACAACAATTGGAAAATTAGCTTACCGATATAAAATGGAAGGTAAAAAAGTAATGTTAGCT
    GCGGGCGATACTTTTAGAGCGGGTGCTATTGATCAATTGAAAGTTTGGGGCGAACGTGTT
    GGTGTAGACGTAATTAGCCAAAGTGAAGGTTCTGATCCAGCTGCTGTTATGTATGATGCG
    ATTAATGCCGCTAAAAACAAAGGTGTTGATATTTTAATCTGTGATACCGCTGGACGTTTA
    CAAAATAAAACAAATCTAATGCAAGAATTAGAAAAAGTTAAGCGTGTAATTAATCGAGCA
    GTGCCAGATGCGCCTCATGAAGCATTACTATGTTTAGATGCTACAACTGGTCAGAATGCG
    TTGTCACAAGCTAGAAACTTTAAAGAAGTAACAAATGTTACAGGTATTGTATTAACGAAA
    TTAGATGGTACAGCCAAAGGTGGTATCGTATTAGCCATTCGTAATGAATTGCACATCCCA
    GTTAAATATGTAGGTTTAGGTGAGCAATTAGATGACTTACAACCATTTAACCCTGAAAGT
    TATGTCTACGGCTTATTCGCTGATATGATTGAACAAAATGAAGAAATAACAACAGTTGAA
    AATGATCAAATTGTAACAGAAGAAAAGGACGATAATCATGGGTCAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1128 [new locus tag: SAUSA300_RS06105 ]
  • symbol: FtsY
  • description: signal recognition particle-docking protein FtsY
  • length: 416
  • theoretical pI: 4.39014
  • theoretical MW: 46590
  • GRAVY: -0.545673

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 388.5)
    and 11 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal recognition particle protein (TIGR00959; HMM-score: 233.6)
    signal recognition particle protein SRP54 (TIGR01425; HMM-score: 185.8)
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 91.4)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 18.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    Signal transduction Regulatory functions Protein interactions LAO/AO transport system ATPase (TIGR00750; EC 2.7.-.-; HMM-score: 16.9)
    arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 16)
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 15.7)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 13.2)
    Unknown function General putative selenium-dependent hydroxylase accessory protein YqeC (TIGR03172; HMM-score: 12.9)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 12)
  • TheSEED  :
    • Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Two cell division clusters relating to chromosome partitioning  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
    and 1 more
    Protein Metabolism Protein biosynthesis Universal GTPases  Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1)
  • PFAM:
    P-loop_NTPase (CL0023) SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 273.5)
    and 16 more
    no clan defined SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 48.9)
    P-loop_NTPase (CL0023) ArgK; ArgK protein (PF03308; HMM-score: 19)
    AAA_30; AAA domain (PF13604; HMM-score: 18.6)
    AAA_17; AAA domain (PF13207; HMM-score: 16.9)
    ABC_tran; ABC transporter (PF00005; HMM-score: 15.5)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 15.2)
    AAA_31; AAA domain (PF13614; HMM-score: 15.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.7)
    AAA_22; AAA domain (PF13401; HMM-score: 14.5)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.3)
    AAA_33; AAA domain (PF13671; HMM-score: 14.1)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.4)
    AAA_24; AAA domain (PF13479; HMM-score: 13.2)
    APS_kinase; Adenylylsulphate kinase (PF01583; HMM-score: 12.8)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 9.5)
    no clan defined Scm3; Centromere protein Scm3 (PF10384; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007941
    • TAT(Tat/SPI): 0.001861
    • LIPO(Sec/SPII): 0.0026
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDANDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQLNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREVIVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVMLAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDTAGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVTGIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQNEEITTVENDQIVTEEKDDNHGSK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_0380(ahpC)alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    SAUSA300_2477(cidC)pyruvate oxidase  [1] (data from MRSA252)
    SAUSA300_0760(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    SAUSA300_1633(gap)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SAUSA300_1201(glnA)glutamine synthetase, type I  [1] (data from MRSA252)
    SAUSA300_1640(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_1149(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SAUSA300_2198(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_2179(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SAUSA300_1306(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_2066(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SAUSA300_2484hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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