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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS02485 [old locus tag: SA0436 ]
- pan locus tag?: SAUPAN002198000
- symbol: SA_RS02485
- pan gene symbol?: dnaX
- synonym:
- product: DNA polymerase III subunit gamma/tau
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1681TTGAATTATCAAGCCTTATATCGTATGTACAGACCCCAAAGTTTCGAGGATGTCGTCGGA
CAAGAACATGTCACGAAGACATTGCGCAATGCGATTTCGAAAGAAAAACAGTCGCATGCT
TATATTTTTAGTGGTCCGAGAGGTACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAA
GCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAA
GGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGC
GTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATAT
AAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCTTTTA
AAGACGTTAGAAGAACCTCCAGCACACGCTATTTTTATATTGGCAACGACAGAACCACAT
AAAATCCCTCCAACAATCATTTCTAGGGCACAACGTTTTGATTTTAAAGCAATTAGCCTA
GATCAAATTGTTGAACGTTTAAAATTTGTAGCAGATGCACAACAAATTGAATGTGAAGAT
GAAGCCTTGGCATTTATCGCTAAAGCGTCTGAAGGGGGTATGCGTGATGCATTAAGTATT
ATGGATCAGGCTATTGCATTTGGTGATGGTACGTTAACATTGCAAGATGCGTTGAATGTC
ACAGGTAGCGTACATGATGAAGCGTTGGATCACTTGTTTGATGATATTGTACAAGGTGAC
GTACAAGCATCTTTTAAAAAATACCATCAGTTTATAACAGAGGGTAAAGAAGTGAATCGC
CTAATAAATGATATGATTTATTTTGTCAGAGATACGATTATGAATAAAACATCTGAGAAA
GATACTGAGTATCGAGCACTGATGAACTTAGAATTAGATATGTTATATCAAATGATTGAT
CTTATTAATGATACATTAGTGTCGATTCGTTTTAGTGTGAATCAAAACGTTCATTTTGAA
GTGTTGTTAGTAAAATTAGCTGAGCAGATTAAGGGTCAACCACAAGTGATTGCGAATGTA
GCTGAACCAGCACAAATTGCTTCATCGCCAAACACAGATGTATTGTTGCAACGTATGGAA
CAGTTAGAGCAAGAATTAAAAACACTAAAAGCACAAGGAGTGAGTGTCGCTCCTGCTCAA
AAATCTTCGAAAAAGCCTGCGAGAGGCATACAAAAATCTAAAAATGCATTTTCAATGCAA
CAAATTGCAAAAGTGCTAGATAAAGCGAATAAGGCAGATATCAAATTGTTGAAAGATCAT
TGGCAAGAAGTGATTGATCATGCCAAAAACAATGATAAAAAATCACTCGTTAGTTTATTG
CAAAATTCGGAACCTGTGGCGGCAAGTGAAGATCACGTACTTGTGAAATTTGAGGAAGAG
ATCCATTGTGAAATCGTCAATAAAGACGACGAGAAACGTAGTAGTATAGAAAGTGTTGTA
TGTAATATCGTTAATAAAAACGTTAAAGTTGTTGGTGTACCATCAGATCAATGGCAAAGA
GTTCGAACGGAGTATTTACAAAATCGTAAAAACGAAGGCGATGATATGCCAAAGCAACAA
GCACAACAAACAGATATTGCTCAAAAAGCAAAAGATCTTTTCGGTGAAGAAACTGTACAT
GTGATAGATGAAGAGTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA_RS02485 [old locus tag: SA0436 ]
- symbol: SA_RS02485
- description: DNA polymerase III subunit gamma/tau
- length: 565
- theoretical pI: 5.32796
- theoretical MW: 63470.6
- GRAVY: -0.422478
⊟Function[edit | edit source]
- reaction: EC 2.7.7.7? ExPASyDNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 461.8)and 11 moreDNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 168.2)Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 40.9)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 40.9)DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 35)Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 32)Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 32)DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 24.9)Protein fate Protein and peptide secretion and trafficking type VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 22)DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 17.1)Cellular processes Sporulation and germination stage V sporulation protein K (TIGR02881; HMM-score: 15.6)Protein fate Degradation of proteins, peptides, and glycopeptides endopeptidase La (TIGR00763; EC 3.4.21.53; HMM-score: 15.3)
- TheSEED: see SA0436
- PFAM: P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 161.9)and 20 moreAAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 54.2)RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 33.5)TIP49; TIP49 C-terminus (PF06068; HMM-score: 28.6)AAA_16; AAA ATPase domain (PF13191; HMM-score: 24.4)post-AAA (CL0604) DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 24)P-loop_NTPase (CL0023) AAA_30; AAA domain (PF13604; HMM-score: 22.9)AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 22.5)AAA_19; AAA domain (PF13245; HMM-score: 21.1)PIF1; PIF1-like helicase (PF05970; HMM-score: 20.4)AAA_22; AAA domain (PF13401; HMM-score: 20.2)AAA_14; AAA domain (PF13173; HMM-score: 20)Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 18.8)AAA_18; AAA domain (PF13238; HMM-score: 18.5)TniB; Bacterial TniB protein (PF05621; HMM-score: 17.7)no clan defined AAA_assoc_2; AAA C-terminal domain (PF16193; HMM-score: 17)P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 16.9)AAA_24; AAA domain (PF13479; HMM-score: 16.9)T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 14.3)RNA_helicase; RNA helicase (PF00910; HMM-score: 12.6)no clan defined Com_YlbF; Control of competence regulator ComK, YlbF/YmcA (PF06133; HMM-score: 7.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.033637
- TAT(Tat/SPI): 0.003109
- LIPO(Sec/SPII): 0.001391
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFSGPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVDEIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPHKIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDALSIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGKEVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVNQNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQGVSVAPAQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKNNDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNVKVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SA_RS06280 (nusA) transcription termination/antitermination protein NusA [2] (data from MRSA252) SA_RS06270 (polC) DNA polymerase III subunit alpha [2] (data from MRSA252) SA_RS00150 DNA polymerase III subunit beta [2] (data from MRSA252) SA_RS00310 aminoglycoside O-nucleotidyltransferase ANT(4')-Ia [2] (data from MRSA252) SA_RS00335 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA [2] (data from MRSA252) SA_RS00690 immunoglobulin G-binding protein A [2] (data from MRSA252) SA_RS01275 formate acetyltransferase [2] (data from MRSA252) SA_RS01960 acetyl-CoA acyltransferase [2] (data from MRSA252) SA_RS02020 single-stranded DNA-binding protein [2] (data from MRSA252) SA_RS02095 alkyl hydroperoxide reductase subunit C [2] (data from MRSA252) SA_RS02120 hypothetical protein [2] (data from MRSA252) SA_RS02145 IMP dehydrogenase [2] (data from MRSA252) SA_RS02490 YbaB/EbfC family nucleoid-associated protein [2] (data from MRSA252) SA_RS02640 ribose-phosphate pyrophosphokinase [2] (data from MRSA252) SA_RS02650 50S ribosomal protein L25/general stress protein Ctc [2] (data from MRSA252) SA_RS02710 cysteine synthase [2] (data from MRSA252) SA_RS02810 pyridoxal 5'-phosphate synthase lyase subunit PdxS [2] (data from MRSA252) SA_RS02860 glutamate--tRNA ligase [2] (data from MRSA252) SA_RS02900 transcription termination/antitermination protein NusG [2] (data from MRSA252) SA_RS02910 50S ribosomal protein L1 [2] (data from MRSA252) SA_RS02915 50S ribosomal protein L10 [2] (data from MRSA252) SA_RS02920 50S ribosomal protein L7/L12 [2] (data from MRSA252) SA_RS02930 DNA-directed RNA polymerase subunit beta [2] (data from MRSA252) SA_RS02935 DNA-directed RNA polymerase subunit beta' [2] (data from MRSA252) SA_RS02950 30S ribosomal protein S7 [2] (data from MRSA252) SA_RS02955 elongation factor G [2] (data from MRSA252) SA_RS02960 elongation factor Tu [2] (data from MRSA252) SA_RS02990 branched chain amino acid aminotransferase [2] (data from MRSA252) SA_RS03070 3-hexulose-6-phosphate synthase [2] (data from MRSA252) SA_RS03155 phosphate acetyltransferase [2] (data from MRSA252) SA_RS03545 hypothetical protein [2] (data from MRSA252) SA_RS03575 transcriptional regulator [2] (data from MRSA252) SA_RS03645 hypothetical protein [2] (data from MRSA252) SA_RS04020 ribosomal subunit interface protein [2] (data from MRSA252) SA_RS04090 thioredoxin reductase [2] (data from MRSA252) SA_RS04140 aldehyde dehydrogenase [2] (data from MRSA252) SA_RS04145 phosphoglycerate kinase [2] (data from MRSA252) SA_RS04150 triose-phosphate isomerase [2] (data from MRSA252) SA_RS04155 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [2] (data from MRSA252) SA_RS04160 enolase [2] (data from MRSA252) SA_RS04330 glycine cleavage system protein H [2] (data from MRSA252) SA_RS04540 D-alanine--poly(phosphoribitol) ligase subunit 2 [2] (data from MRSA252) SA_RS04575 NADH dehydrogenase [2] (data from MRSA252) SA_RS04680 glucose-6-phosphate isomerase [2] (data from MRSA252) SA_RS04785 beta-ketoacyl-[acyl-carrier-protein] synthase II [2] (data from MRSA252) SA_RS04845 tryptophan--tRNA ligase [2] (data from MRSA252) SA_RS04935 hypothetical protein [2] (data from MRSA252) SA_RS05190 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [2] (data from MRSA252) SA_RS05295 phosphocarrier protein HPr [2] (data from MRSA252) SA_RS05300 phosphoenolpyruvate--protein phosphotransferase [2] (data from MRSA252) SA_RS05330 hypothetical protein [2] (data from MRSA252) SA_RS05350 pyruvate dehydrogenase E1 component subunit alpha [2] (data from MRSA252) SA_RS05355 pyruvate dehydrogenase E1 component subunit beta [2] (data from MRSA252) SA_RS05360 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [2] (data from MRSA252) SA_RS05620 thiol reductase thioredoxin [2] (data from MRSA252) SA_RS05860 cell division protein FtsZ [2] (data from MRSA252) SA_RS05895 isoleucine--tRNA ligase [2] (data from MRSA252) SA_RS05945 carbamoyl-phosphate synthase large chain [2] (data from MRSA252) SA_RS06085 beta-ketoacyl-ACP reductase [2] (data from MRSA252) SA_RS06140 50S ribosomal protein L19 [2] (data from MRSA252) SA_RS06165 succinyl-CoA ligase subunit beta [2] (data from MRSA252) SA_RS06170 succinyl-CoA ligase subunit alpha [2] (data from MRSA252) SA_RS06225 30S ribosomal protein S2 [2] (data from MRSA252) SA_RS06235 elongation factor Ts [2] (data from MRSA252) SA_RS06490 glutamine synthetase [2] (data from MRSA252) SA_RS06690 transketolase [2] (data from MRSA252) SA_RS06730 aconitate hydratase [2] (data from MRSA252) SA_RS06860 aminoacyltransferase FemB [2] (data from MRSA252) SA_RS07005 cold-shock protein CspA [2] (data from MRSA252) SA_RS07065 2-oxoglutarate dehydrogenase E1 component [2] (data from MRSA252) SA_RS07360 nucleoside-diphosphate kinase [2] (data from MRSA252) SA_RS07385 DNA-binding protein HU [2] (data from MRSA252) SA_RS07400 30S ribosomal protein S1 [2] (data from MRSA252) SA_RS07605 phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating) [2] (data from MRSA252) SA_RS07695 elongation factor P [2] (data from MRSA252) SA_RS07700 peptidase M24 family protein [2] (data from MRSA252) SA_RS07730 glycine dehydrogenase [2] (data from MRSA252) SA_RS07820 superoxide dismutase [2] (data from MRSA252) SA_RS07880 glycine--tRNA ligase [2] (data from MRSA252) SA_RS07955 molecular chaperone DnaK [2] (data from MRSA252) SA_RS07985 30S ribosomal protein S20 [2] (data from MRSA252) SA_RS08135 hypothetical protein [2] (data from MRSA252) SA_RS08295 50S ribosomal protein L21 [2] (data from MRSA252) SA_RS08435 trigger factor [2] (data from MRSA252) SA_RS08505 aldehyde dehydrogenase [2] (data from MRSA252) SA_RS08545 isocitrate dehydrogenase (NADP(+)) [2] (data from MRSA252) SA_RS08550 citrate synthase [2] (data from MRSA252) SA_RS08560 pyruvate kinase [2] (data from MRSA252) SA_RS08625 universal stress protein UspA [2] (data from MRSA252) SA_RS08630 acetate kinase [2] (data from MRSA252) SA_RS08675 30S ribosomal protein S4 [2] (data from MRSA252) SA_RS08730 serine protease [2] (data from MRSA252) SA_RS08760 formate--tetrahydrofolate ligase [2] (data from MRSA252) SA_RS08935 proline dehydrogenase [2] (data from MRSA252) SA_RS09005 transaldolase [2] (data from MRSA252) SA_RS09445 glucosamine-6-phosphate isomerase [2] (data from MRSA252) SA_RS09710 glutamine amidotransferase [2] (data from MRSA252) SA_RS09810 non-heme ferritin [2] (data from MRSA252) SA_RS09860 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C [2] (data from MRSA252) SA_RS10535 molecular chaperone GroEL [2] (data from MRSA252) SA_RS11010 uracil phosphoribosyltransferase [2] (data from MRSA252) SA_RS11060 aldehyde dehydrogenase family protein [2] (data from MRSA252) SA_RS11070 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [2] (data from MRSA252) SA_RS11075 fructose-bisphosphate aldolase [2] (data from MRSA252) SA_RS11145 purine-nucleoside phosphorylase [2] (data from MRSA252) SA_RS11245 glutamine--fructose-6-phosphate aminotransferase [2] (data from MRSA252) SA_RS11430 Asp23/Gls24 family envelope stress response protein [2] (data from MRSA252) SA_RS11605 50S ribosomal protein L13 [2] (data from MRSA252) SA_RS11630 50S ribosomal protein L17 [2] (data from MRSA252) SA_RS11640 30S ribosomal protein S11 [2] (data from MRSA252) SA_RS11670 50S ribosomal protein L15 [2] (data from MRSA252) SA_RS11680 30S ribosomal protein S5 [2] (data from MRSA252) SA_RS11705 50S ribosomal protein L5 [2] (data from MRSA252) SA_RS11735 30S ribosomal protein S3 [2] (data from MRSA252) SA_RS11745 30S ribosomal protein S19 [2] (data from MRSA252) SA_RS11750 50S ribosomal protein L2 [2] (data from MRSA252) SA_RS11755 50S ribosomal protein L23 [2] (data from MRSA252) SA_RS13340 pyruvate oxidase [2] (data from MRSA252) SA_RS13375 hydroxymethylglutaryl-CoA synthase [2] (data from MRSA252) SA_RS13730 class I fructose-bisphosphate aldolase [2] (data from MRSA252) SA_RS13735 malate:quinone oxidoreductase [2] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
Mol Microbiol: 2002, 43(6);1387-400
[PubMed:11952893] [WorldCat.org] [DOI] (P p) - ↑ 2.000 2.001 2.002 2.003 2.004 2.005 2.006 2.007 2.008 2.009 2.010 2.011 2.012 2.013 2.014 2.015 2.016 2.017 2.018 2.019 2.020 2.021 2.022 2.023 2.024 2.025 2.026 2.027 2.028 2.029 2.030 2.031 2.032 2.033 2.034 2.035 2.036 2.037 2.038 2.039 2.040 2.041 2.042 2.043 2.044 2.045 2.046 2.047 2.048 2.049 2.050 2.051 2.052 2.053 2.054 2.055 2.056 2.057 2.058 2.059 2.060 2.061 2.062 2.063 2.064 2.065 2.066 2.067 2.068 2.069 2.070 2.071 2.072 2.073 2.074 2.075 2.076 2.077 2.078 2.079 2.080 2.081 2.082 2.083 2.084 2.085 2.086 2.087 2.088 2.089 2.090 2.091 2.092 2.093 2.094 2.095 2.096 2.097 2.098 2.099 2.100 2.101 2.102 2.103 2.104 2.105 2.106 2.107 2.108 2.109 2.110 2.111 2.112 2.113 2.114 2.115 2.116 2.117 2.118 2.119 2.120 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)