From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02420 [old locus tag: SAUSA300_0452 ]
  • pan locus tag?: SAUPAN002198000
  • symbol: SAUSA300_RS02420
  • pan gene symbol?: dnaX
  • synonym:
  • product: DNA polymerase III subunit gamma/tau

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02420 [old locus tag: SAUSA300_0452 ]
  • symbol: SAUSA300_RS02420
  • product: DNA polymerase III subunit gamma/tau
  • replicon: chromosome
  • strand: +
  • coordinates: 509330..511027
  • length: 1698
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    TTGAATTATCAAGCCTTATATCGTATGTACAGACCCCAAAGTTTCGAGGATGTCGTCGGA
    CAAGAACATGTCACGAAGACATTGCGCAATGCGATTTCGAAAGAAAAACAGTCGCATGCT
    TATATTTTTAGTGGTCCGAGAGGTACGGGGAAAACGAGTATTGCCAAAGTGTTTGCTAAA
    GCAATCAACTGTCTAAATAGCACTGATGGAGAACCTTGTAATGAATGTCATATTTGTAAA
    GGCATTACGCAGGGGACTAATTCAGATGTGATAGAAATTGATGCTGCTAGTAATAATGGG
    GTTGATGAAATAAGAAATATTAGAGACAAAGTTAAATATGCACCAAGTGAATCGAAATAT
    AAAGTTTATATTATAGATGAGGTGCACATGCTAACAACAGGTGCTTTTAATGCCCTTTTA
    AAGACGTTAGAAGAACCTCCAGCACACGCTATTTTTATATTGGCAACGACAGAACCACAT
    AAAATCCCTCCAACAATCATTTCTAGGGCACAACGTTTTGATTTTAAAGCAATTAGCCTA
    GATCAAATTGTTGAACGTTTAAAATTTGTAGCAGATGCACAACAAATTGAATGTGAAGAT
    GAAGCCTTGGCATTTATCGCTAAAGCGTCTGAAGGGGGTATGCGTGATGCATTAAGTATT
    ATGGATCAGGCTATTGCATTTGGTGATGGTACGTTAACATTGCAAGATGCGTTGAATGTC
    ACAGGTAGCGTACATGATGAAGCGTTGGATCACTTGTTTGATGATATTGTACAAGGTGAC
    GTACAAGCATCTTTTAAAAAATACCATCAGTTTATAACAGAAGGTAAAGAAGTGAATCGC
    CTAATAAATGATATGATTTATTTTGTCAGAGATACGATTATGAATAAAACATCTGAGAAA
    GATACTGAGTATCGAGCACTGATGAACTTAGAATTAGATATGTTATATCAAATGATTGAT
    CTTATTAATGATACATTAGTGTCGATTCGTTTTAGTGTGAATCAAAACGTTCATTTTGAA
    GTGTTGTTAGTAAAATTAGCTGAGCAGATTAAGGGTCAACCACAAGTGATTGCGAATGTA
    GCTGAACCAGCACAAATTGCTTCATCGCCAAACACAGATGTATTGTTGCAACGTATGGAA
    CAGTTAGAGCAAGAACTAAAAACACTAAAAGCACAAGGAGTGAGTGTCGCTCCTGTTCAA
    AAATCTTCGAAAAAGCCTGCGAGAGGCATACAAAAATCTAAAAATGCATTTTCAATGCAA
    CAAATTGCAAAAGTGCTAGATAAAGCGAATAAGGCAGATATCAAATTGTTGAAAGATCAT
    TGGCAAGAAGTGATTGATCATGCCAAAAATAATGATAAAAAATCACTCGTTAGTTTATTG
    CAAAATTCGGAACCTGTGGCGGCAAGTGAAGATCACGTACTTGTGAAATTTGAGGAAGAG
    ATCCATTGTGAAATCGTCAATAAAGACGACGAGAAACGTAGTAGTATAGAAAGTGTTGTA
    TGTAATATCGTTAATAAAAACGTTAAAGTTGTTGGTGTACCATCAGATCAATGGCAAAGA
    GTTCGAACGGAATATTTACAAAATCGTAAAAACGAAGGCGATGATATGCCAAAGCAACAA
    GCACAACAAACAGATATTGCTCAAAAAGCAAAAGATCTTTTCGGTGAAGAAACTGTACAT
    GTGATAGATGAAGAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1698

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS02420 [old locus tag: SAUSA300_0452 ]
  • symbol: SAUSA300_RS02420
  • description: DNA polymerase III subunit gamma/tau
  • length: 565
  • theoretical pI: 5.32796
  • theoretical MW: 63498.7
  • GRAVY: -0.41823

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 461.8)
    and 10 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta' subunit (TIGR00678; EC 2.7.7.7; HMM-score: 168.2)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 41.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease LonB (TIGR02902; EC 3.4.21.-; HMM-score: 41.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 35)
    Cellular processes Cellular processes Sporulation and germination ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 31)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides ATP-dependent protease, Lon family (TIGR02903; EC 3.4.21.-; HMM-score: 31)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 24.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 22)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 17.1)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein K (TIGR02881; HMM-score: 15.6)
  • TheSEED: see SAUSA300_0452
  • PFAM:
    P-loop_NTPase (CL0023) DNA_pol3_delta2; DNA polymerase III, delta subunit (PF13177; HMM-score: 161.9)
    and 20 more
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 54.2)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 33.5)
    TIP49; TIP49 C-terminus (PF06068; HMM-score: 28.6)
    post-AAA (CL0604) DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III (PF12169; HMM-score: 24.3)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 24.3)
    AAA_30; AAA domain (PF13604; HMM-score: 22.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 22.5)
    AAA_19; AAA domain (PF13245; HMM-score: 20.5)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 20.4)
    AAA_14; AAA domain (PF13173; HMM-score: 20)
    AAA_22; AAA domain (PF13401; HMM-score: 20)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 18.8)
    AAA_18; AAA domain (PF13238; HMM-score: 18.4)
    TniB; Bacterial TniB protein (PF05621; HMM-score: 17.7)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 17.3)
    no clan defined AAA_assoc_2; AAA C-terminal domain (PF16193; HMM-score: 17.1)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 16.9)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 14.4)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 12.6)
    no clan defined Com_YlbF; Control of competence regulator ComK, YlbF/YmcA (PF06133; HMM-score: 7.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.033637
    • TAT(Tat/SPI): 0.003109
    • LIPO(Sec/SPII): 0.001391
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFSGPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVDEIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPHKIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDALSIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGKEVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVNQNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQGVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKNNDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNVKVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_RS02420&oldid=71747"